ブレインビジョン株式会社

ヒトiPS細胞由来心筋スフェロイドを用いた膜電位とカルシウムの同時マッピング

sakuraba — Thu, 22 Jan 2026 07:51:59 +0000

移植片とホスト組織間の電気的結合の評価にも最適

2Dから3Dへ：より生体に近い心臓モデルでの研究を

現在、心血管疾患は世界の主要な死因であり、多大な医療・経済的負担となっています。しかし、膨大な研究費が投じられているにもかかわらず、新規薬剤の90%が臨床試験で失敗しており、その主な原因の一つとして臨床的・亜臨床的な心毒性が挙げられます。

長年、心臓研究の主流であった従来の2D培養モデルには以下の欠点があります。

2D培養モデルの欠点

構造的複雑性の欠如
生体内の心臓が持つ複雑な3D構造や、細胞外マトリックス（ECM）との相互作用を再現できない
生理学的関連性の低さ
細胞同士の立体的な接触や微細環境が異なるため、薬物応答の予測能に限界がある

研究現場には、ヒト特有の心臓組織の発生、生理、疾患プロセスをより正確に模倣できる「次世代の3Dモデル」が求められています。

心筋スフェロイド：3Dがもたらす高い生理学的妥当性

ヒトiPS細胞（hiPSC）から作製される心臓スフェロイド（またはオルガノイド）は、心筋細胞、内皮細胞、線維芽細胞などが自己組織化することによって3D構造を形成したミニチュアモデルです。

心筋スフェロイドを使うメリット

生体に近い組織構成
3D環境下で細胞が相互作用し、ヒト特有の心臓組織の成熟や疾患プロセスを高い精度で再現
予測能の向上
創薬、毒性試験、疾患モデリングにおいて、従来の2Dモデルを遥かに凌駕する生理学的関連性を示す
柔軟な設計
特定の疾患患者由来のiPSCを用いることで、パーソナライズされた治療法の開発や、特定の遺伝的背景を持つ疾患（肥大型心筋症や不整脈源性心筋症など）のモデリングが可能

革新的な「オールオプティカル」電気生理学プラットフォーム

最新の研究では、光遺伝学（オプトジェネティクス）による刺激と、膜電位（Vm）およびカルシウムトランジェント（CaT）の同時光学マッピングを組み合わせた「オールオプティカル」な手法が確立され、心筋スフェロイドにも応用されています。

下の画像は、ヒトiPS細胞由来心筋スフェロイドを膜電位感受性の色素 (RH237)とカルシウム感受性蛍光タンパク質 (jRCaMP1b)で標識し、2つの高速カメラで完全同期撮影した例です。

ヒトiPS細胞由来心筋スフェロイドを用いた膜電位とカルシウムの同時マッピング例

「オールオプティカル」システムがもたらすメリット

非侵襲的かつ高精度な記録
電気刺激によるノイズや電極の物理的干渉を排除し、組織に触れることなくミリ秒単位の電気生理現象を可視化できる
高精度な可視化
電気的マッピングと比較して、高い時空間解像度で心臓の電気生理学的機能を直接イメージングができる
興奮収縮連関の統合的理解
膜電位（Vm）とカルシウムトランジェント（CaT）を同一のサンプルから同時に記録することで、心機能や不整脈メカニズムについて、より多くの情報に基づいた洞察を得ることできる
独立した刺激
光遺伝学アクチュエータ（CheRiffなど）を移植片（グラフト）側のみに発現させることで、ホスト組織に影響を与えずグラフトのみを独立して刺激することが可能
活性化の明確な帰属
遺伝子にコードされたセンサーと有機色素を使い分けることで、観察された電気的活動がグラフト由来なのかホスト由来なのかを曖昧さなく判別できる

出典：J Mol Cell Cardiol. 2025 Feb:199:51-59.

サンプル動画

サンプル	ヒトiPS細胞由来心筋スフェロイド
刺激方法	電気刺激
蛍光プローブ	膜電位感受性色素 (RH237、左画像) カルシウム感受性蛍光タンパク質 (jRCaMP1b、右画像)
イメージングシステム	MiCAM03-N256 2カメラシステム
画素数	256x256
フレームレート	500fps (2.0msec/frame)
提供	Dr. Hanyu Zhang, Dr. Bijay Guragain, Dr. Jianyi Zhang, and Dr. Jack M. Rogers Department of Biomedical Engineering The University of Alabama at Birmingham
参考論文	Optogenetic stimulation and simultaneous optical mapping of membrane potential and calcium transients in human engineered cardiac spheroids Journal of Molecular and Cellular Cardiology 199 (2025) 51–59

3Dスフェロイドだからこそ可能な高度な解析

スフェロイドを使ったこの手法は、単なる記録ツールにとどまりません。3D心臓モデルの特性を最大限に活かした解析を支援します。

スフェロイドを使ったオールオプティカルシステムがもたらすメリット

再生医療における「Host-Graft Coupling」の評価
移植されたスフェロイド（グラフト）とホスト組織を独立して刺激し、その電気的結合の状態を明確に追跡ができる
興奮収縮連関の深い理解
同一の細胞群から膜電位とカルシウムダイナミクスを同時に取得することで、リエントリーや伝導遅延といった複雑な現象を多角的に評価することが可能
高度な安全性試験
2Dモデルでは見逃されていた微細な薬物応答や心毒性を、より生体に近い3D環境で検出可能

参考商品

上の動画撮影に使用された高速イメージングシステム MiCAM03-N256をメインとする光学マッピングシステムです。

蛍光染色された心臓・神経サンプルの電気的活動をで1,818フレーム/秒ほどの高速で捉えて画像化し、画像解析・波形解析～マップ画像作成・エクスポートまで対応しています。データ計測に必要な高輝度LED光源、電気刺激装置も付属。すぐに実験が始められるオールインワンのターンキーシステムです。

高速光学マッピングシステム

主なアプリケーション

膜電位感受性色素やGEVIを用いた膜電位イメージング
カルシウム色素やGECIを用いたカルシウムイメージング
2カメラを用いたレシオイメージング

構成と特長

高速カメラ

画素数：256x256～32x32画素
最大フレームレート： 1,818fps (256x256画素)、20,000fps (32x32画素)
心電図などのアナログ信号記録やパルス出力、光源点灯信号などが可。外部機器との同期も簡単

→ 高速イメージングシステム　MiCAM03-N256

ソフトウェア

Activation mapやConduction velocityなど、様々なマップ作成機能
直感的で簡単な操作・分析時間の短縮
論文・プレゼンテーション用の高解像度イメージをエクスポート可能

→ Optical Mappingデータ解析ソフトウェア　BV Workbench

マクロ蛍光顕微鏡

0.19x～6.3xほどの低倍率なのに他社製蛍光顕微鏡より明るく観察
高速蛍光イメージングのS/N比が向上
蛍光スプリッターで2波長同時計測も可能

→ マクロ蛍光顕微鏡　THT Mesoscope

高輝度LED光源

高輝度照明　イメージングのS/N比が向上
高安定　生物シグナルにノイズを加えない

→ マルチLED光源システム　LEX9

多機能電気刺激装置

パルス出力、アナログ入力、オシロスコープ、光源点灯制御など
刺激アイソレータ内蔵　電極を接続すればすぐに刺激可能

→　アイソレーター内蔵多機能電気刺激装置

実績

販売開始から27年の間に世界230の脳科学/心臓研究機関へ約430台が販売され、MiCAMシリーズは高速膜電位イメージングの標準機として認められています。

弊社製品の使用した学術論文は27年で約1,000報、発表されています。

→ Google Sholarで論文リストを見る

お問い合わせ

心筋スフェロイドを用いたマッピングシステムの導入、およびマッピングシステムの詳細仕様や価格に関するお問い合わせは、以下のボタンより承っております。貴分野の研究をより発展させるお手伝いとなるソリューションをご提案いたします。

お問い合わせ

その他の参考論文：

Cardiac organoids: a new tool for disease modeling and drug screening applications
Front Cardiovasc Med. 2025 May 20:12:1537730.

第72回日本実験動物学会総会にて機器展示を行います。

sakuraba — Wed, 21 May 2025 02:37:36 +0000

2025年 5月 21日（水）～ 5月 23日（金）　名古屋国際展示場　ポートメッセなごやにて開催されます第72回日本実験動物学会総会で機器展示を行います。

大会名	第72回日本実験動物学会総会
日程	2025年 5月21日（水）～ 5月23日（金）
会場	名古屋国際展示場　ポートメッセなごや〒455-0848 愛知県名古屋市港区金城ふ頭2丁目2
ブース番号	M-8

中国Beijing Yeeran Technology製小動物用超音波イメージング装置の製品ページをアップしました

sakuraba — Mon, 12 May 2025 04:55:19 +0000

小動物用超音波エコー

小動物用ポータブルエコー VINNO 6 LAB

小動物用高解像度エコー ULTIMUS 9LAB

内因性信号イメージングデータと論文一覧

sakuraba — Thu, 19 Sep 2024 07:00:42 +0000

マウス In Vivo脳　内因性信号イメージング (MiCAM ULTIMA-H)

サンプル	マウス In Vivo脳（体性感覚野バレル皮質）
方法	ピエゾを使用したヒゲ刺激
イメージングシステム	MiCAM ULTIMA
フレームレート	100fps (10msec/frame)
画素数	100x100
提供	Dr. Isabelle Ferezou and Dr. Carl C.H. Petersen, Brain Mind Institute, EPFL, Switzerland

内因性信号イメージング（Intrinsic Optical Signal Imaging）

Acute Ablation of Cortical Pericytes Leads to Rapid Neurovascular Uncoupling.

Kassandra Kisler, Angeliki M. Nikolakopoulou, Melanie D. Sweeney, Divna Lazic, Zhen Zhao, and Berislav V. Zlokovic
Front Cell Neurosci. 2020; 14: 27.

Temporal refinement of sensory-evoked activity across layers in developing mouse barrel cortex.

Alexander van der Bourg, Jenq-Wei Yang, Maik C Stüttgen, Vicente Reyes-Puerta, Fritjof Helmchen, Heiko J Luhmann
Eur J Neurosci. 2019 Sep;50(6):2955-2969.

In vivo imaging and analysis of cerebrovascular hemodynamic responses and tissue oxygenation in the mouse brain.

Kassandra Kisler, Divna Lazic, Melanie D Sweeney, Shane Plunkett, Mirna El Khatib, Sergei A Vinogradov, David A Boas, Sava Sakadži & Berislav V Zlokovic
Nat Protoc . 2018 Jun;13(6):1377-1402.

Pericyte degeneration leads to neurovascular uncoupling and limits oxygen supply to brain.

Kassandra Kisler, Amy R Nelson, Sanket V Rege, Anita Ramanathan, Yaoming Wang, Ashim Ahuja, Divna Lazic, Philbert S Tsai, Zhen Zhao, Yi Zhou, David A Boas, Sava Sakadžić & Berislav V Zlokovic
Nat Neurosci. 2017 Jan 30. doi: 10.1038/nn.4489.

Probing the functional impact of sub-retinal prosthesis.

Sébastien Roux, Frédéric Matonti, Florent Dupont, Louis Hoffart, Sylvain Takerkart, Serge Picaud, Pascale Pham, and Frédéric Chavane
eLife. 2016; 5: e12687. Published online 2016 Aug 23. doi: 10.7554/eLife.12687

Susceptibility of Primary Sensory Cortex to Spreading Depolarizations.

Volodymyr B. Bogdanov, Natalie A. Middleton, Jeremy J. Theriot, Patrick D. Parker, Osama M. Abdullah, Y. Sungtaek Ju, Jed A. Hartings, and K.C. Brennan
J Neurosci. 2016 Apr 27;36(17):4733-43. doi: 10.1523/JNEUROSCI.3694-15.2016.

Orofacial Neuropathic Pain Leads to a Hyporesponsive Barrel Cortex with Enhanced Structural Synaptic Plasticity.

Karine Thibault, Sébastien Rivière, Zsolt Lenkei, Isabelle Férézou, and Sophie Pezet
PLoS One. 2016 Aug 22;11(8):e0160786. doi: 10.1371/journal.pone.0160786. eCollection 2016.

Sensory-Evoked Intrinsic Imaging Signals in the Olfactory Bulb Are Independent of Neurovascular Coupling.

Roberto Vincis, Samuel Lagier, Dimitri Van De Ville, Ivan Rodriguez and Alan Carleton
Cell Rep. 2015 Jul 14;12(2):313-25. doi: 10.1016/j.celrep.2015.06.016. Epub 2015 Jul 2.

フラビン蛋白蛍光イメージング

Numerical Analysis of Microcoil-Induced Electric Fields and Evaluation of In vivo Magnetic Stimulation of the Mouse Brain.

Shunsuke Sugai, Hisaya Higuchi, Jun Nishikawa, Kazuo Satoh, Shuichi Murakami, Takashi Tateno
IEEJ Transactions on Electrical and Electronic Engineering, 15(11), 1672–1680.

Potentiating a non-neuronal cardiac cholinergic system reinforces the functional integrity of the blood brain barrier associated with systemic anti-inflammatory responses.

Shino Oikawa, Yuko Kai, Asuka Mano, Shuei Sugama, Naoko Mizoguchi, Masayuki Tsuda, Kazuyo Muramoto, Yoshihiko Kakinuma
Brain, Behavior, and Immunity 81 (2019) 122-137

Sound- and current-driven laminar profiles and their application method mimicking acoustic responses in the mouse auditory cortex in vivo.

Shuto Muramatsu, Masato Toda, Jun Nishikawa, Takashi Tateno
Brain Res. 2019 Oct 15;1721:146312.

In vivo transcranial flavoprotein autofluorescence imaging of tonotopic map reorganization in the mouse auditory cortex with impaired auditory periphery.

Kengo Takasu, Takashi Tateno
Hear Res. 2019 Jun;377:208-223.

Micromagnetic Stimulation of the Mouse Auditory Cortex In Vivo Using an Implantable Solenoid System.

Shunsuke Minusa, Hisayuki Osanai and Takashi Tateno
IEEE Trans Biomed Eng. 2018 Jun;65(6):1301-1310.

Micro-coil-induced Inhomogeneous Electric Field Produces sound-driven-like Neural Responses in Microcircuits of the Mouse Auditory Cortex In Vivo.

Hisayuk Osanai, Shunsuke Minusa and Takashi Tateno
Neuroscience. 2018 Feb 10;371:346-370.

Flavoprotein fluorescence imaging-based electrode implantation for subfield-targeted chronic recording in the mouse auditory cortex.

Jun Nishikawa, Yuto Ohtaka, Yuishi Tachibana, Yasutaka Yanagawa, Hisayuki Osanai, Takeaki Haga and Takashi Tateno
J Neurosci Methods. 2018 Jan 1;293:77-85.

Salicylate-induced frequency-map reorganization in four subfields of the mouse auditory cortex.

Yasutaka Yanagawa, Kengo Takasu, Hisayuki Osanai, Takashi Tateno
Available online 10 June 2017

Spatiotemporal dynamics of functional clusters of neurons in the mouse motor cortex during a voluntary movement.

Riichiro Hira, Fuki Ohkubo, Katsuya Ozawa, Yoshikazu Isomura, Kazuo Kitamura, Masanobu Kano, Haruo Kasai and Masanori Matsuzaki
J Neurosci. 2013 Jan 23;33(4):1377-90. doi: 10.1523/JNEUROSCI.2550-12.2013.

GCaMPイメージングデータと論文一覧

sakuraba — Thu, 19 Sep 2024 06:54:52 +0000

In Vivo GCaMPイメージング (MiCAM02-HR)

論文一覧

論文名	サンプル	種類	機器名
Fast scanning high optical invariant two-photon microscopy for monitoring a large neural network activity with cellular resolution. Keisuke Ota, Yasuhiro Oisi, Takayuki Suzuki, Muneki Ikeda, Yoshiki Ito, Tsubasa Ito, Kenta Kobayashi, Midori Kobayashi, Maya Odagawa, Chie Matsubara, Yoshinori Kuroiwa, Masaru Horikoshi, Junya Matsushita, Hiroyuki Hioki, Masamichi Ohkura, Junichi Nakai, Masafumi Oizumi, Atsushi Miyawaki, Toru Aonishi, Takahiro Ode, Masanori Murayama bioRxiv 2020.07.14.201699.	Cortex	G-CaMP7.09 or GCaMP6f	MiCAM ULTIMA THT Mesoscope
Astrocytes Modulate Baroreflex Sensitivity at the Level of the Nucleus of the Solitary Tract. Svetlana Mastitskaya, Egor Turovsky, Nephtali Marina, Shefeeq M. Theparambil, Anna Hadjihambi, Sergey Kasparov, Anja G. Teschemacher, Andrew G. Ramage, Alexander V. Gourine and Patrick S. Hosford J Neurosci. 2020 Apr 8; 40(15): 3052-3062.	Brainstem	GCaMP6	MiCAM02 HR
Detecting mTBI by Learning Spatio-temporal Characteristics of Widefield Calcium Imaging Data Using Deep Learning. Fatemeh Koochaki, Foroogh Shamsi, Laleh Najafizadeh 2020 42nd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC)..	Cerebral cortex	GCaMP6s	MiCAM ULTIMA
Distinct Contributions of Whisker Sensory Cortex and Tongue-Jaw Motor Cortex in a Goal-Directed Sensorimotor Transformation. Johannes M. Mayrhofer, Sami El-Boustani, Georgios Foustoukos, Matthieu Auffret, Keita Tamura, Carl C.H.Petersen Volume 103, Issue 6, 25 September 2019, Pages 1034-1043.e5	Cortex	GCaMP6f	MiCAM ULTIMA
Decoding cortical brain states from widefield calcium imaging data using visibility graph. Li Zhu, Christian R. Lee, David J. Margolis and Laleh Najafizadeh Biomed Opt Express. 2018 Jul 1; 9(7): 3017-3036.	Cortex	GCaMP6f	MiCAM ULTIMA
Odor representations in the olfactory bulb evolve after the first breath and persist as an odor afterimage. Michael Andrew Patterson, Samuel Lagier and Alan Carleton Proc Natl Acad Sci U S A. 2013 Aug 27;110(35):E3340-9.	Olfactory bulb	GCaMP3	MiCAM ULTIMA
Visuomotor learning promotes visually evoked activity in the medial prefrontal cortex Andrew J Peters, Andrada-Maria Marica, Julie M J Fabre, Kenneth D Harris, Matteo Carandini Cell Rep. 2022 Oct 18; 41(3): 111487.	Cortex	GCaMP6s	THT Mesoscope
Neural signatures of auditory hypersensitivity following acoustic trauma Matthew McGill Ariel E Hight, Yurika L Watanabe, Aravindakshan Parthasarathy, Dongqin Cai, Kameron Clayton, Kenneth E Hancock, Anne Takesian, Sharon G Kujawa, and Daniel B Polley eLife. 2022; 11: e80015.	Auditory cortex	GCaMP6s	THT Mesoscope
Striatal activity topographically reflects cortical activity Andrew J Peters, Julie MJ Fabre, Nicholas A Steinmetz, Kenneth D Harris, and Matteo Carandini Nature. 2021 Mar 1; 591(7850): 420?425.	Cortex	GCaMP6s	THT Mesoscope
Cellular and Widefield Imaging of Sound Frequency Organization in Primary and Higher Order Fields of the Mouse Auditory Cortex Sandra Romero, Ariel E Hight, Kameron K Clayton, Jennifer Resnik, Ross S Williamson, Kenneth E Hancock, and Daniel B Polley Cereb Cortex. 2020 Mar; 30(3): 1603?1622.	Auditory cortex	GCaMP6s	THT Mesoscope
A suite of transgenic driver and reporter mouse lines with enhanced brain cell type targeting and functionality Tanya L. Daigle, Linda Madisen, Travis A. Hage, Matthew T. Valley, Ulf Knoblich, Rylan S. Larsen, Marc M. Takeno, Lawrence Huang, Hong Gu, Rachael Larsen, Maya Mills, Alice Bosma-Moody, LafAkea Siverts, Miranda Walker, Lucas T. Graybuck, Zizhen Yao, Olivia Fong1 Thuc Nghi Nguyen, Emma Garren, Garreck Lenz, Mariya Chavarha, Julie Pendergraft, James Harrington, Karla E. Hirokawa, Julie A. Harris, Philip R. Nicovich, Medea McGraw, Douglas R. Ollerenshaw, Kimberly Smith, Christopher A. Baker, Jonathan T. Ting, Susan M. Sunkin, Jerome Lecoq, Michael Z. Lin, Edward S. Boyden, Gabe J. Murphy, Nuno da Costa, Jack Waters, Lu Li, Bosiljka Tasic, and Hongkui Zeng Cell. 2018 Jul 12; 174(2): 465?480.e22.	Cortex	GCaMP6s GCaMP6f	THT Mesoscope
Aberrant Cortical Activity in Multiple GCaMP6-Expressing Transgenic Mouse Lines Nicholas A. Steinmetz, Christina Buetfering, Jerome Lecoq, Christian R. Lee, Andrew J. Peters, Elina A. K. Jacobs, Philip Coen, Douglas R. Ollerenshaw, Matthew T. Valley, Saskia E. J. de Vries, Marina Garrett, Jun Zhuang, Peter A. Groblewski, Sahar Manavi, Jesse Miles, Casey White, Eric Lee, Fiona Griffin, Joshua D. Larkin, Kate Roll, Sissy Cross, Thuyanh V. Nguyen, Rachael Larsen, Julie Pendergraft, Tanya Daigle, Bosiljka Tasic, Carol L. Thompson, Jack Waters, Shawn Olsen, David J. Margolis, Hongkui Zeng, Michael Hausser, Matteo Carandini, and Kenneth D. Harris eNeuro. 2017 Sep-Oct; 4(5): ENEURO.0207-17.2017.	Cortex	GCaMP6f	THT Mesoscope
An extended retinotopic map of mouse cortex Jun Zhuang, Lydia Ng, Derric Williams, Matthew Valley, Yang Li, Marina Garrett, and Jack Waters eLife. 2017; 6: e18372.	Cortex	GCaMP6s GCaMP6f	THT Mesoscope

テクニカルガイド | MiCAMにおける膜電位光測定のノイズに関する考察と可能性

sakuraba — Wed, 18 Sep 2024 08:50:17 +0000

（注：この記事は1998年に執筆されたものです。）

CCDなどの電子的なカメラで撮影した映像信号には、様々な雑音が含まれます。そのうち計測上、最も大きく影響するのは、ショットノイズです。ショットノイズの源は光量子であるので、これを取り除くことは不可能です。

次に影響するのは、暗電流ノイズで、熱揺らぎや電気的な不確定性により発生します。膜電位の光計測では多くの場合、暗電流ノイズはショットノイズに比べ小さいので、注意すべきはショットノイズだけです。

ショットノイズは光量子がセンサーで光電変換される時に発生します。例えば、平均的に、1個の画素にある時間で降り注ぐ光量子数が10000個であっても、確率的な分布があるので、量子個数の平方根に比例して揺らぎます。すなわち、10000の平方根は100ですから、概ね、9950個から10050個の間で揺らいでいます。これは物理的な現象であって、どのようなセンサーであっても変わることはありません。このため、膜電位感受性色素のように変化量が少ない場合は極めて大きく結果に影響が現れます。0.1%の変化を正確に記録するためには、最低でも１画素につき100万個の光量子が必要であると言えます。

そこで、1画素が扱う光量子の数を増やすには、光電変換によって発生した電荷の画素に貯まる電荷量を増やす方法が有効です。半導体に形成できる容量は面積に比例するので、大きな画素ほど大量の電荷が蓄積でき、ノイズが小さいということが言えます。つまり、大きな画素のセンサーに明るい照明を用いるならノイズ性能は向上します。

逆に言うと、輝度が十分でない限り、どのようなセンサーを用いても微少な変化を高解像に高速に捕らえることは不可能です。ところが、照明には限界があります。特に蛍光観察の場合は光源に相当に大きな電力を用いても、輝度が不足することが多く、大きな画素のセンサーに十分な光を照射できないのが普通です。光源に300W以上の巨大なランプを用いて無理に輝度を強めると、光路中のフィルタやレンズなどが破損することもあり、普通の光学機器では蛍光輝度を10倍に高めることは非常に難しいでしょう。

これまで光計測の主流の考え方は、光を強め、センサーを大きくしてショットノイズを小さくするという直線的なものでした。吸光の場合には容易に達成でき、これを実現したのは、私たちが電総研で1991年に完成したMOSセンサーを用いた光計測装置です。

しかし、蛍光の場合、この装置を効率よく使用するには、光学系や染色などについて相当の配慮が必要で、不可能ではありませんが、困難であると言えました。そして、最終的に問題となるのは、蛍光色素の脱色の問題です。RH-７９５色素の場合では、１秒程度の記録でも脱色が顕著で、系統的に安定な記録をすること難しく、一発勝負でデータを取る必要がありました。つまり、安定な計測を望む限り、輝度はそれほど明るくできないというのが結論です。従って、大きなセンサーの利点を十分に生かすことはできないのです。

では、高解像と高速を両立した蛍光計測はできないのでしょうか？結論から言えば、これを解決する手法はアベレージングと信号処理です。１回の計測ではノイズに埋もれて見えない現象でも16回や64回のアベレージングによって見えます。アベレージングが嫌われるのは繰り返し起こせない現象には適応できないからです。当然ですが、もし１回の試行だけで計測する必要があるならば、アベレージングはできません。しかしながら、もし、”起こせない”としても”起きる”ことが加算計測できるならばアベレージングの適応範囲は広がります。

例えば、自発発火の状態を計測することを考えましょう。ある細胞が自発発火しているとき、この自発発火に関連している他の神経の活動を計測するという課題です。一見するとこれは単発現象でありアベレージングは不可能に見えます。しかし、細胞活動を電極でユニット観察できるならば、アベレージングが可能になります。つまり、ユニットの記録を、増幅して映像記録装置のトリガに入力します。このトリガの前後の映像をメモリに記録しアベレージングすれば、非同期のノイズは減少し、この細胞に同期した信号が強調されます。

ここで重要なのは、トリガのタイミングの前後というところです。トリガの前とは、この細胞を発火せしめる活動に関連しており、後とはこの細胞の発火が影響したことに関連する可能性があります。トリガの前と後を記録するという意味で、プレーポストトリガ機能といわれるものですが、この機能を装備するかどうかが分かれ目です。信号処理も重要で、ショットノイズの性質を利用したノイズ除去アルゴリズムを適応するとショットノイズを若干改善することが可能です。

我々が開発したMiCAMの標準カメラでは、画素当り約100,000個の電荷が蓄積されます。従って、ショットノイズは飽和照度を100%として0.3%程度です。実験的に多くの試料で、16回程度のアベレージングと信号処理によって蛍光色素を用いた膜電位変化の観察の可能なことが確認されています。重要なのは、計測において光学系や光源が極めてシンプルであることです。光学系については5倍以上の倍率を利用するなら、普通の蛍光顕微鏡がほぼそのまま使用可能です。光源は150Wのハロゲンランプで十分です。輝度が普通なので長時間の連続照射が可能で、電磁シャッターなしでも可能です。（電磁シャッターの使用を推奨しますが。。。。）

低倍では、明るいマクロ蛍光顕微鏡が必要ですが、市販のレンズを組み合わせて容易に作ることができます。低倍率の光学系とのマッチングから見ると、MiCAMの標準センサーは小型過ぎるという場合があります。そこで、速度的には3msec程度に制限されますが、1/2や2/3インチセンサーを用いたカメラも順次提供して行きます。

MiCAMの単純な電気的性能は必ずしも世界最高ではありません。（例えば、PixelVision社の超高速カメラの方が多くの点で優秀であることを認めます。）ですが、MiCAMは実験で使えることは間違いありませんし、全体バランスのとれた装置であると自負しています。生理実験に必要な機能を統合しているので、多くの研究者に目的の実験に最も近いソルーションを提供できると考えています。

追記：MiCAMをお使いになって、もし10倍のS/Nが必要だとお感じになられたら、同時に100倍の輝度が必要なことをお考えください。そして、その光源が10倍の安定度を要求することと、色素の脱色が10倍*から100倍の速さで進むことをお考えください。

テクニカルガイド | 神経活動を光で追う

sakuraba — Wed, 18 Sep 2024 08:45:25 +0000

1. 神経活動の観察手段としての膜電位感受性色素の特徴

本論に入る前に、脳・神経の活動を観察する方法について考えてみよう。神経活動の実体は神経細胞の膜電位(membrane potential)の急激な変化であるが、観察手法によって、個々の膜電位を反映した観察ができるものから、分解能は良くなくても全体的な内容が把握できる方法が開発されてきた。

まず、測定の対象である神経活動と膜電位について簡単に説明しておこう。神経細胞はイオンの濃度差をポテンシャルにし電気的に分極している。静止状態では約60mVの電位差で、細胞の内側は外側に比べ負に分極している。これを過分極状態といい、その電位を静止電位という。神経が活動すると、NaやCaイオンが細胞内に流入し、電位の逆転が起こり、瞬間的(1msec程度)に細胞内の電位は約50mV正に分極する。これを脱分極といい、この瞬間的な電位を活動電位あるいはスパイクという。また、神経活動が神経細胞から神経細胞に伝播されるとやや遅い(10msec程度)シナプス電位が発生するが、その大きさは約20mVである。

図1に示すのは、神経活動計測の代表的な方法とその特徴をまとめた図である。どの方法も神経活動を計測する手段ではあるが、観察できる内容は微妙に異なる。図1Aの内部電極法はガラスピペットにKClなどの電解質をつめた電極を、文字どおり細胞内に刺入することで、神経細胞の膜電位を計測する方法である。したがって、最も精度が高い計測が可能である。

そのような神経細胞のすぐ側に金属製の電極を当てると、スパイクの発生に伴い生じる局所イオン電流を観察できる。それが図1Bの外部電極法であり、タングステンワイヤーなどを研磨した先端の鋭い電極を用いて、単一の神経細胞の活動が計測できる。内部電極に比べて絶対精度は低下するが、同じ電極を動かすことによって、多数の神経細胞からの活動を観察することが可能である。おもに、生きた動物の脳活動を観察するのに用いられる。

それに対して、図1Cのように先端を丸めた電極を脳の表面やスライスの表面に密着させると、近傍の多くの神経細胞から生じるイオン電流の和が観察できる。この表面電極法が良いところは、内部・外部電極法は非常に位置に敏感で一般にむずかしい技術であるのに対して、位置に鈍感で、取扱いが簡単なところである。しかし、細胞レベルでの時間・空間分解能はないという問題点がある。

そして、図1Dの膜電位感受性色素(voltage sensitive dye)を用いた光学的方法がこの節の主題である。上記の説明のごとく、神経細胞の活動とは電気的変化であるので、細胞膜を電圧感受性色素と呼ばれる特殊な色素で染め、電気変化を光変化に変換し映像で捉えることができる。

もともとの神経活動は前述の活動電位(100mV/1msec)とシナプス電位(20mV/10msec)であり、両者が混ざった膜電位変化を色素のはたらきで可視化することができる。図1Eに示したのは、ヤリイカ巨大神経軸際の活動電位を内部電極法(実線)と膜電位感受性色素の光量変化を重ねてプロット(離散点)した図である。このように、単一神経細胞膜を対象とする場合、両者は相対的に非常に良く一致する。つまり、この手法の最大の利点は計測している内容は、内部電極法が測定している直接の神経活動であるということであり、一般的に難しく、熟練を要する電極刺入にかかわる困難さがなく、セットアップさえ整えば初心者でも簡単に再現性の高い観測が可能であることである。しかも、光学的な倍率(対物レンズの焦点距離)によって、また、試料によっても状況は異なるが、細胞レベルの信号を2次的に1,000点以上同時観察できる唯一の方法である。

しかし、良いところばかりではない。この方法の最大の問題点は、電圧感受性色素による光量変化が非常に小さい(0.1～1%)ため、ノイズが多いことである。この問題点については後で詳しく議論するが、実用上はさまざまな計測範囲の制限として現れる。たとえば、細胞レベルの分解能があるといっても、1つ1つの細胞を特定して観察するには、最低でも10回以上の平均加算により雑音を低減する必要がある。また、生きた動物に適応する場合、鼓動や呼吸により振動が及ぼすノイズが大きな問題となる。

図1 神経活動計測の手法

図は神経膜の一部を拡大して、模式的に示したイメージ図である。内部電極法(A)は、神経膜を貫くガラス電極により神経膜電位を直接観察する。それに対し外部電極法(BとC)では、神経興奮によるイオン電流を細胞外から観察する。Bは神経膜に接触して活動電位単位の記録をするもので、先端の鋭いタングステン電極などが用いられ、Cのように銀などの先端を丸めたものを外部電極として用いると集合電位(電流)が観察できる。膜電位感受性色素(D)は、分子プローブであり、神経膜に入り込んで膜電位を蛍光強度に変換する。Eに示した図は、ヤリイカ巨大神経軸策の活動電位であり、黒の実線が内部電極による測定、赤のドットが膜電位感受性色素による測定結果である。

以上のように、電圧感受性色素を用いた光学測定法は、電極法などと同様に神経活動そのもの(電気信号)を実時間で観察する手段であり、電極法では不可能な多点計測を実現する手法であるといえる。この適応範囲を示すのが図2である。

光学系の倍率を変えることで、器官レベルから細胞以下のレベルまでを適応範囲にできる。おそらく、現状で最も盛んなのは、1～3倍のレンズ系で、神経回路網(neural network)のはたらきを観察することであり、未知の神経回路を簡単に観察することができる。たとえば、遺伝子に欠陥を与えたモデル動物(ノックアウトマウスなど)の未知の脳を観察するという研究には強力な武器になると期待されている。

また、それより高い倍率(10倍以上)の光学系で、神経細胞1個の内部で電気信号がどのように発生し、伝播するかという研究も地味でむずかしい課題であるが、神経細胞の機能分子などのはたらきを解明するうえで重要である。逆に、1倍以下の低い倍率のレンズによって、脳の表面を露出させた動物などを用いた研究の成果も著しく、脳内部でのダイナミックな情報表現の解明はこの手法の独壇場である。また脳神経に限らず、心筋や副腎の電気的興奮を観察する有効な手段として応用が始まっている。

図2 膜電位感受性色素を用いた光計測の適応範囲

光学系の倍率と観察対象を対比した図である。縮小系のレンズ(A, 0.2～1倍)では脳全体や脳の一部が、低倍率レンズ(B, 1～3倍)では、神経回路網、さらに倍率を上げると、細胞1個(C)や、シナプス部分までの解像力(D)が得られる。

2. 計測の問題点―ノイズと信号

前項で述べたように、この手法は非常に有用であり、それほど難しい技術でもない。しかしながら、多くの知識とノウハウがなければ十分な計測結果は得られない。たとえば、ノイズを小さくしたいのであれば、光を明るくすれば当然よい。しかし、強烈な照明は、色素の変性と試料のダメージを誘起して、結果として正しい計測が果たせないのである。膜電位感受性色素を用いた計測で良い結果を得るには、いかに照明を抑え、いかに染色を抑え、それでいて、いかに明るい映像を捉えることを可能にするか、という問題にこたえることである。当然、そこには大いなる努力に基づいた妥協が必要である。

まず、ノイズと信号、光強度の関係を図3に示したので参照してほしい。この図の横軸は色素の発する蛍光の総量で、縦軸は蛍光の変化成分であり、どちらも対数軸である。数値はわれわれの用いているセンサーの1個のピクセル（約35μm角）に露光される光の光量子数である。2本の45度の黒の実線は、神経の活動によって代表的な膜電位感受性色素（Di4-ANEPPS）が変化する信号を示し、上が１％のラインで、下が0.1％のラインである。

試料によって信号の大きさにはバリエーションがあるが、多くの場合、0.2％とか、0.6％といった値になり、この2本の直線の間に入る。計測装置自身がもっているノイズは一般に光量とは無関係なので、赤の点線で示したような水平な直線になる。これより、暗い領域の観察はほとんど無理であるが、多くの場合、このラインを超えるのは容易である。実際上、最も問題となるノイズは、赤の破線で示した光量の平方根で増加する光ショットノイズである。光ショットノイズは、光の粒子性（光子）に基づく雑音であり、物理的にさけようがないノイズである。このラインの上ならば、信号がノイズを上回り、S/N比は1以上ということになる。図中＊で示した領域が、実質的にS/N＝1程度の領域である。当然、これより右の明るい領域ならば、より良いS/N比で計測が可能である。

ところが、問題はそのような明るい光学系（蛍光顕微鏡）が存在しないことである。明るくするためには照明を強くすることになるが、試料に対するダメージが大きくなる。ごく一般的な蛍光顕微鏡などで得られる蛍光量は、図3右下のeasyという矢印が示す範囲で、特別に開口率の高いレンズを工夫した場合に、high NAの矢印で示す蛍光量が実現できる。それより、明るいものは現状では存在せず、明るくするためには照明の光源を強くする以外にない。

普通、100～150Wのハロゲン電球を光源に用いるが、500W級の光源に変更しても、3～5倍程度の輝度向上であり、S/Nの改善は平方根なのでせいぜい2倍程度である。ところが、試料のダメージは照明に対し線形かそれ以上に悪化するので、経験的に10倍程度は悪化する。つまり、2倍の改善を10倍の犠牲で得ることになる。もちろん、1回だけでも高いS/N比で観察ができれば目的を達成できるという実験ならば、躊躇なく強烈な光源を用意すべきだろう。しかし、多くの場合は、長時間安定な計測を前提にした実験の組み立てになるのも事実である。

図3 光計測の信号量とノイズの検討図

横軸はセンサーの1画素に飛び込む蛍光量を示し、縦軸はその変化成分である。2本の実線が信号であり、破線がノイズである。ノイズより信号が上回る領域（＊印）ではS/N=1程度の観察が可能である。

我々の研究室で経験的に見出したラット・マウス大脳スライス標本で有効な染色法を紹介する。

経験的に見出された有効な染色法

Di4-ANEPPS（5mg）のビンに、1mlのアルコールを直接入れて、よく溶解する。
（カタログにはメタノールとあるが、エタノールの方がよく溶ける）
そこに、クレモフォア－EL10％水溶液を0.5ml加えて、よく溶解してストック液とする。冷蔵庫で2～3か月は保存可能。
上記のストック液35μmを0.5ml仔ウシ血清と0.5mlのACSFに溶かして、染色液にする。最終濃度は約0.1mg/mlである。
スライスは、インターフェースチェンバーのような状態を保つことができる密閉型の染色容器を作り、上記の染色液をスライスの表面に少量ずつ滴下して、約20分間静置する。温度は室温、容器内部でACSFを5％二酸化炭素・酸素ガスをパブリングして、酸素と湿気を保つ。
スライスを観察チェンバー（サブマージュ型、顕微鏡の下に設置）に運び、約30分間インキュベートしてから観察に入る。
この方法で、約4時間程度は安定な観察ができる。

3. 明るい光学系と高速カメラシステム

光学系の明るさは、おもに対物レンズの開口数（NA）による。開口数が倍になると、受光系で4倍、照明系で4倍、合計で16倍明るい映像が撮影できる。つまり、明るさは開口数の4乗に比例する。

開口数が高いレンズとは、大口径で焦点距離が短いということである。したがって、被写体から広がる光を広角に捉えるので明るい。市販の顕微鏡レンズの場合、40倍で0.7程度、20倍で0.3程度、10倍では0.1程度のものが一般的である。しかし、図2のように、最も用途の多い神経回路の観察などでは、1～3倍の低倍の光学系が必要になる。

多くの顕微鏡メーカーの販売する2倍レンズの開口数は0.05程度で、きわめて暗い。そこで、われわれは図4に示したような特別な光学系を作製して使用している。対物レンズはニコン製の一眼レフレンズ（F1.2、50mm 標準レンズ）を解体して余分な部品（絞りや焦点調整機構）を取り除きケースに収め再組立てしたものを使用している。このレンズの開口数は約0.7である。市販の顕微鏡レンズに比べて、200倍明るい映像が得られる。

この自作の顕微鏡は、高い開口数のレンズを2つ用いて、いわゆる無限遠設計になっている。ダイクロイックミラーの置かれる本体部分はほぼ平行な光束が通り、投影レンズを経てCCDセンサーに結像される。投影レンズにはライカ製の80mmの実体顕微鏡レンズを用いている。総合倍率は約1.6倍である。590㎚以上の長波長のみを通す吸収フィルターを配置した。

図4　光学系は開口数が大きな対物レンズを中心に組み上げた自家製の同軸落射蛍光顕微鏡である。黄色の線で示したのが照明光であり、赤で示したのが蛍光光である。両者の分離にはダイクロイックミラーを用いる。この顕微鏡が明るい理由は、カメラレンズ（Nikon F1.2, 50mm）を解体して作った対物レンズにある。その下に試料である脳のスライスを置き、電極などをセットする。総合倍率は約1.6倍。

照明系には市販の150Wハロゲン電球を用いたファイバー照明器具（モリテックス製、MHF-G150）を用いている。色素の波長特性に合わせるため、照明は干渉フィルターを通し、緑色光（530nm）にしている。ダイクロイックミラーは、530nmの光を90％以上反射し、試料に投下される。試料での蛍光のうち、590～650nmの光が電圧感受成分なので、これを効果的に切り出すため、ダイクロイックミラーは590～650nmの光を90％以上透過し、その後、迷走光を遮るため590nm以上の長波長のみを通す吸収フィルターを配置した。

カメラシステムは、われわれの研究室とブレインビジョン社で開発した高速CCD撮影システムである。このカメラに使用しているCCDセンサーはSONYのICX076という通常用途の白黒CCDイメージセンサーである。普通は、7MHz程度のクロックで動作させるべき部品であるが、強引に50MHzで動作させ、さらに、縦方向（V方向）のCCD転送クロックを4回連続で与え、縦の走査線数を60本にまとめることで高速に動作させている。結果として、約700μsecで全画面の読み出しが可能である。神経活動が1msec程度であることを考慮すると700μsec必ずしも十分な速度ではないが、おおむね要求を満たす速度である。

このCCDセンサーは1/5インチサイズで、有効受光面はおおむね3mm×2mmほどである。小型なため、蓄積可能な電荷量が少ないのがやや不満な点であるが、ここまで高速動作が可能なのは、小さいためなので納得している。CCDセンサーで捉えられた映像は高速ADCを経て、デジタル処理によって加工・保存される。映像データは一時的にメモリーに貯え、計測直後にPCIバスを介してホストコンピュータに転送される。

4. 計測結果

前項で紹介したカメラシステムと光学系を用いて、脳を生きたままスライスして得た試料をDi-4-ANEPPS色素で染色して、小さな電気刺激を与えたときの観測結果を図5に示す。

図5Aに示したのは、摘出したラット脳に対する観測部位と電極などの配置を示した図である。観測領域は海馬の中でもCA1と呼ばれる部分である。刺激電極をCA1領域のおもな入力線維であるシェーファー側枝に配置し、短いパルスで電気刺激する。刺激はシェーファー側枝を通じて、CA1の錐体細胞を次々に興奮させていく。その様子が、図5Cからよくわかる。

この映像は、もともと0.75msec単位で測定されているが、図の都合で1枚おき、すなわち1.5msecごとの映像を示した。本来、膜電位感受性色素の神経興奮による変化は0.3％程度なので、コンピュータ処理により変化成分だけを約300倍に強調し、赤い色をつけて映像にスーパーインポーズして示した。赤の濃い部分がより脱分極している部位である。このように、実時間で膜電位を2次元でイメージングできるのが、この方法の最大のメリットである。

さらに、図5Bに示したのは、スライスの任意の点（この場合CA1の錐体細胞の各場所に当たる4ヵ所）を選び、その点での神経活動を時間方向に解析した表示である。これはちょうど、複数の内部電極を刺したときとほぼ等価であるが、実際には、同時に映像として記録した各画素のデータを読み取ったものである。つまり、この画像上の任意の点に内部電極が存在していると思えばよい。

図5Bで、4つのトレースの下に示したノイズの少ない実線は、内部電極で記録した活動電位であり、赤い小さな丸で示したのは、ほぼ同じ場所の光計測の結果である。このように、ほとんど同じ波形が得られることがわかる。しかし、よく見ると、活動電位のピークに比較して、遅い成分が光計測ではやや大きめに写る。これには、①光で観察される活動電位はいくつかの細胞の平均値なので、速い活動電位は同期性が悪く、実際よりも小さめに観察される、②膜電位感受性色素の測定量は膜電位と同様に膜の密度にも比例する。

この試料では、神経細胞膜の密度がデンドライトで高く、細胞体で低いため、デンドライトの成分が混じるとシナプス電位成分が強調される、という2つの理由が考えられる。膜電位感受性色素を用いた研究では、組織による染色性の違いやアクティブな膜の密度について考慮する必要がある。なお、一部色素（たとえば、RH-155）は、神経細胞よりもグリアによく取り込まれるため、グリアの比較的遅い分極がデータに混入することが知られている。この測定に用いたDi-4-ANEPPSは、神経細胞の活動に依存する蛍光変化がほぼ全てであり、グリア活動はほとんど見えない。まったく新しい試料の場合には、使用する色素との相性などを調べてから詳しい実験に進むべきである。

図5　ラット脳より海馬を摘出し、約400μmの厚みにスライスし、Di4-ANEPPSにて染色した。観察部位はCA1（図Ａを参照）であり、刺激はCA3からCA1に投射するシェーファー側枝に電流を流す。図Bに示したのは、図Aに線で示された各部分の画素の蛍光変化量を時間でプロットしたものである。図B下のパネルに示したのは内部電極で計った膜電位（実線）とほぼ同じ場所の光計測の点（赤丸）を重ねて示している。Cは、各図の左下の時間（msec単位）の映像を並べたものである。この図のデータは、8回のアベレージングとS/N改善のためのフィルター処理を施してある。

おわりに

膜電位感受性色素による神経興奮活動の計測は、いくつかのポイントをつかめば、難しい方法ではない。また、この技術は歴史的には古く、すでに開発されて20年が経過している。しかしながら、いまだに一般に難しい技術と考えられている。その理由は、計測に適したカメラシステムが入手困難なことと、システマティックに安定な計測を実現する地道なデータの積み重ねが後になり、派手な結果を求めてきたためではないかと反省する。

われわれの研究の1つの方向性は、誰でも可能な光計測技術を完成することである。それと同期して、世界中の研究機関で、GFP（クラゲ由来の緑色蛍光タンパク質）をチャネルタンパク質に遺伝子レベルで導入する研究も進展している。近い将来、生まれながらに電飾することなく膜電位で蛍光変化する脳をもつ実験動物が誕生する可能性がある。そうなれば、一気に普及することになるだろう。

さて、今のところ、ヒトを対象とした医療診断に実施例はない。その最大の理由は、MRIのようにまったく無侵襲な手法と異なり、診断する部位が見える状態でないとならないからである。また、色素の毒性も少ないとはいえ、安全とは言い切れない。しかしながら、米国の医科系大学を中心に心臓手術や脳外科手術の現場で確度を高めるための補助的な手段としての応用を検討していると聞く。何年か先には、多くの生命を救える手法に発展するかもしれない。その日の到来をねがって、本節の結びとしたい。

(出典：共立出版「光による医学診断」(2001年3月30日出版))

テクニカルガイド | ランゲンドルフ灌流心の膜電位光学計測

sakuraba — Wed, 18 Sep 2024 08:21:32 +0000

(The Columbus Dispatch, Ohio State research could help atrial-fibrillation patients, 2017/2/5より引用)

膜電位光学計測って何ですか？

細胞膜電位の変化を光量を細かく測定することにより計測する技法です。

特殊な蛍光色素（膜電位感受性色素）で組織を染色すると、膜電位変化に応じて蛍光量が僅かに変化します。その蛍光量変化を特殊な高速カメラで捉えるのが膜電位光学計測です。その色素の応答速度は1ミリ秒以下なのでリアルタイムの膜電位計測が可能です。

電極での膜電位計測と何が違うのですか？

計測点の数とデータの質が異なります。

細胞膜電位の変化に応じて細胞膜上の蛍光色素が蛍光量を変化させるため、その光量変化を時系列で表現するとパッチクランプと同様の波形になります。したがって、膜電位光学計測では、1画素の視野中に含まれる細胞群のパッチクランプデータを平均化したような結果が得られます。
また電極法では数百点の同時計測は困難であり、主として細胞外電位が計測されますが、膜電位光学計測では数万点以上のパッチクランプ電極を敷き詰めて同時計測したようなデータが得られます。

心房の膜電位変化は計測できますか？

心房の膜電位も光学計測できます。

マウスの心房だけでなく培養心筋細胞シートやiPS細胞由来心筋細胞シートの膜電位動態も単離心臓同様に光学計測できます。強力な励起光で背景蛍光量を上げるほど良好なS/N比で計測できます。

どんなカメラでも膜電位の光学計測が可能ですか？

膜電位光学計測には特殊なイメージセンサーを持つ高速度カメラが必要です。

膜電位以外に何か計測できますか？: ブレインビジョン社の光学計測装置は2カメラ、または4カメラまで同期させて高速度撮影できます。近年では膜電位感受性色素と細胞内Ca²⁺指示薬Rhod2-AMで単離心臓を2重染色した後、膜電位と細胞内Ca²⁺動態を同時計測する技法が欧米では一般的なものになっています。膜電位や細胞内Ca²⁺動態以外の組織や細胞の状態をリアルタイムに表現できる蛍光プローブの活用を希望される声が少なくなく、溶存酸素濃度やATPなどの多様なプローブの実用化が期待されています。

（ヒト単離心臓の膜電位と細胞内Ca²⁺動態の光学計測例（ワシントン大学より））

心室細動の発生前後の膜電位動態は記録できますか？

非常に簡単にできます。

細動誘導プログラムを起動して記録を開始すると、ペーシングと細動誘導が自動実行されます。細動が発生するまで、誘導刺激タイミングの設定を変更しながら計測を繰り返すだけで、細動発生前後の心臓全体の膜電位動態が記録できます。

ラットの心室細動が誘発される前後の膜電位動態。

188ｘ160点の膜電位動態を500fpsで記録した例。記録後に任意の点の膜電位動態を光量変化-時間軸の波形で表すことが可能。データ解析は直感的な操作が可能なソフトウェアによりプレゼン資料用データまで迅速に作成可能。等時図やConduction Velocityの算出、空間的周波数特性の解析、各種APD解析など、ブレインビジョン社のデータ解析ソフトウェアは多様な機能が活用できる。

参考動画

Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart

Multiparametric Optical Mapping of the Langendorff-perfused Rabbit Heart

Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart

High-resolution Optical Mapping of the Mouse Sino-atrial Node

論文一覧 | 単離心臓・培養心筋細胞 | 膜電位感受性色素

sakuraba — Wed, 18 Sep 2024 06:32:38 +0000

単離心臓（ランゲンドルフ灌流心）

合計：244

論文名	サンプル	色素	イメージングシステム
Identification of an endogenous glutamatergic transmitter system controlling excitability and conductivity of atrial cardiomyocytes. Duanyang Xie, Ke Xiong, Xuling Su, Guanghua Wang, Qiang Ji, Qicheng Zou, Lingling Wang, Yi Liu, Dandan Liang, Jinfeng Xue, Luxin Wang, Xueting Gao, Xingdong Gu, Hongyu Liu, Xiaoyu He, Li Li, Jian Yang, Youming Lu, Luying Peng & Yi-Han Chen Cell Research volume 31, pages951?964 (2021)	Isolated heart	VSD (RH-237)	MiCAM ULTIMA
α1-adrenergic stimulation increases ventricular action potential duration in the intact mouse heart. William Joyce, Koen T. Scholman, Bjarke Jensen, Tobias Wang, and Bastiaan J. Boukens FACETS. 6(): 823-836.	Isolated heart	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Proinflammatory Cytokine Modulates Intracellular Calcium Handling and Enhances Ventricular Arrhythmia Susceptibility. Yung-Nan Tsai, Ya-Wen Hsiao, Shien-Fong Lin, Yi-Hsin Chan, Yu-Cheng Hsieh, Wei-Hua Tang, An-Sheng Lee, Yu-Ting Huang, Hsing-Yuan Li, Tze-Fan Chao, Satoshi Higa, Tsu-Juey Wu, Shih-Lin Chang, Shih-Ann Chen Front Cardiovasc Med. 2021 Mar 16;8:623510.	Isolated heart	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM02 (dual camera)
Adult zebrafish ventricular electrical gradients as tissue mechanisms of ECG patterns under baseline vs. oxidative stress. Yali Zhao, Nicholas A James, Ashraf R Beshay, Eileen E Chang, Andrew Lin, Faiza Bashar, Abram Wassily, Binh Nguyen, Thao P Nguyen Cardiovasc Res. 2021 Jul 7;117(8):1891-1907.	Isolated heart (zebrafish)	VSD (Di-4-ANEPPS)	MiCAM03
Lack of authentic atrial fibrillation in commonly used murine atrial fibrillation models. Fumin Fu, Michael Pietropaolo, Lei Cui, Shilpa Pandit, Weiyan Li, Oleg Tarnavski, Suraj S. Shetty, Jing Liu, Jennifer M. Lussier, Yutaka Murakami, Prabhjit K. Grewal, Galina Deyneko, Gordon M. Turner, Andrew K.P. Taggart, M. Gerard Waters, Shaun Coughlin, Yuichiro Adachi bioRxiv 2021.08.17.456655;	Isolated heart	VSD (Di-4-ANEPPS)	MiCAM03
High Level of Real Urban Air Pollution Promotes Cardiac Arrhythmia in Healthy Mice. Hyewon Park, Sangchul Lim, Seunghoon Lee, Dasom Mun, JiYoung Kang, Hyoeun Kim, Hyelim Park, Changsoo Kim, Sunho Park, Yeong Min Lim, Boyoung Joung Korean Circ J. 2021 Feb;51(2):157-170.	Isolated heart	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Adherens junction engagement regulates functional patterning of the cardiac pacemaker cell lineage. Kandace Thomas, Trevor Henley, Simone Rossi, M Joseph Costello, William Polacheck, Boyce E Griffith, Michael Bressan Dev Cell. 2021 May 17;56(10):1498-1511.e7.	Isolated heart (chick embryo)	VSD (Di-4-ANEPPS) CaSD (Cal-520)	MiCAM ULTIMA
Theoretical Models and Computational Analysis of Action Potential Dispersion for Cardiac Arrhythmia Risk Stratification. Uma Mahesh R. Avula, Lea Melki, Jared S. Kushner, Stephanie Liang, and Elaine Y. Wan Front Cardiovasc Med. 2021; 8: 649489.	Isolated heart	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Role of Reduced Sarco-Endoplasmic Reticulum Ca²⁺-ATPase Function on Sarcoplasmic Reticulum Ca²⁺ Alternans in the Intact Rabbit Heart. Lianguo Wang, Rachel C. Myles, I-Ju Lee, Donald M. Bers, and Crystal M. Ripplinger Front Physiol. 2021; 12: 656516.	Isolated heart	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Fully implantable and bioresorbable cardiac pacemakers without leads or batteries. Yeon Sik Choi, Rose T Yin, Anna Pfenniger, Jahyun Koo, Raudel Avila, K Benjamin Lee, Sheena W Chen, Geumbee Lee, Gang Li, Yun Qiao, Alejandro Murillo-Berlioz, Alexi Kiss, Shuling Han, Seung Min Lee, Chenhang Li, Zhaoqian Xie, Yu-Yu Chen, Amy Burrell, Beth Geist, Hyoyoung Jeong, Joohee Kim, Hong-Joon Yoon, Anthony Banks, Seung-Kyun Kang, Zheng Jenny Zhang, Chad R Haney, Alan Varteres Sahakian, David Johnson, Tatiana Efimova, Yonggang Huang, Gregory D Trachiotis, Bradley P Knight, Rishi K Arora, Igor R Efimov, John A Rogers Nat Biotechnol. 2021 Jun 28.	Isolated heart	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Flexible and Transparent Metal Nanowire Microelectrode Arrays and Interconnects for Electrophysiology, Optogenetics, and Optical Mapping. Zhiyuan Chen, Nicolas Boyajian, Zexu Lin, Rose T. Yin, Sofian N. Obaid, Jinbi Tian, Jaclyn A. Brennan, Sheena W. Chen, Alana N. Miniovich, Leqi Lin, Yarong Qi, Xitong Liu, Igor R. Efimov, Luyao Lu Adv. Mater. Technol. 2021, 6, 2100225.	Isolated heart	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Rutaecarpine targets hERG channels and participates in regulating electrophysiological properties leading to ventricular arrhythmia. Ge Zhan, Fang Wang, Yun-qi Ding, Xiang-hua Li, Yue-xin Li, Zheng-rong Zhao, Jia-xin Li, Yan Liu, Xin Zhao, Cai-chuan Yan, Bao-xin Li J Cell Mol Med. 2021;00:1?12.	Isolated heart	VSD (RH-237)	MiCAM05
Knockout of interleukin-17A diminishes ventricular arrhythmia susceptibility in diabetic mice via inhibiting NF-κB-mediated electrical remodeling. De-sheng Li, Gen-long Xue, Ji-ming Yang, Chang-zhu Li, Rui-xin Zhang, Tao Tian, Zheng Li, Ke-wei Shen, Yang Guo, Xue-ning Liu, Jin Wang, Yan-jie Lu & Zhen-wei Pan Acta Pharmacologica Sinica (2021)	Isolated heart	VSD (RH-237)	MiCAM05
Anti-arrhythmic and inotropic effects of empagliflozin following myocardial ischemia. Mohammed Ali Azam, Praloy Chakraborty, Daoyuan Si, BeiBei Du, Stéphane Massé, Patrick F H Lai, Andrew C T Ha, Kumaraswamy Nanthakumar Life Sci. 2021 Jul 1;276:119440.	Isolated heart	CaSD (Rhod-2 AM)	MiCAM ULTIMA
NLRP3 inflammasome is a key driver of obesity-induced atrial arrhythmias. Larry Scott Jr, Anke C Fender, Arnela Saljic, Luge Li, Xiaohui Chen, Xiaolei Wang, Dominik Linz, Jilu Lang, Mathias Hohl, Darragh Twomey, Thuy T Pham, Rodrigo Diaz-Lankenau, Mihail G Chelu, Markus Kamler, Mark L Entman, George E Taffet, Prashanthan Sanders, Dobromir Dobrev, Na Li Cardiovasc Res. 2021 Jun 16;117(7):1746-1759.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Minimally invasive system to reliably characterize ventricular electrophysiology from living donors. Aida Oliván-Viguera, María Pérez-Zabalza, Laura García-Mendívil, Konstantinos A. Mountris, Sofía Orós-Rodrigo, Estel Ramos-Marquès, José María Vallejo-Gil, Pedro Carlos Fresneda-Roldán, Javier Fañanás-Mastral, Manuel Vázquez-Sancho, Marta Matamala-Adell, Fernando Sorribas-Berjón, Javier André Bellido-Morales, Francisco Javier Mancebón-Sierra, Alexánder Sebastián Vaca-Núñez, Carlos Ballester-Cuenca, Miguel Ángel Marigil, Cristina Pastor, Laura Ordovás, Ralf Köhler, Emiliano Diez & Esther Pueyo Scientific Reports volume 10, Article number: 19941 (2020).	Tissue slice (human)	VSD (RH-237)	MiCAM05
Thioridazine Induces Cardiotoxicity via Reactive Oxygen Species-Mediated hERG Channel Deficiency and L-Type Calcium Channel Activation. Yan Liu, Xueqi Xu, Yuhao Zhang, Mingzhu Li, Jiamengyi Guo, Caichuan Yan, Fang Wang, Yuexin Li, Yunqi Ding, Baoxin Li, and Pan Fan Oxidative Medicine and Cellular Longevity, vol. 2020, Article ID 3690123, 17 pages, 2020.	Isolated heart (mouse)	VSD (RH-237)	MiCAM ULTIMA
Identification of transmembrane protein 168 mutation in familial Brugada syndrome. Akio Shimizu, Dimitar P Zankov, Akira Sato, Masahiro Komeno, Futoshi Toyoda, Satoru Yamazaki, Toshinori Makita, Taichi Noda, Masahito Ikawa, Yoshihiro Asano, Yohei Miyashita, Seiji Takashima, Hiroshi Morita, Taisuke Ishikawa, Naomasa Makita, Masahito Hitosugi, Hiroshi Matsuura, Seiko Ohno, Minoru Horie, Hisakazu Ogita FASEB J. 2020 May;34(5):6399-6417.	Isolated heart (mouse)	VSD (RH-237)	MiCAM03
Innervation and Neuronal Control of the Mammalian Sinoatrial Node: A Comprehensive Atlas. Peter Hanna, Michael J. Dacey, Jaclyn Brennan, Alison Moss, Shaina Robbins, Sirisha Achanta, Natalia P. Biscola, Mohammed A. Swid, Pradeep S. Rajendran, Shumpei Mori, Joseph E. Hadaya, Elizabeth H. Smith, Stanley G. Peirce, Jin Chen, Leif A. Havton, Zixi (Jack) Cheng, Rajanikanth Vadigepalli, James Schwaber, Robert L. Lux, Igor Efimov, John D. Tompkins, Donald B. Hoover, Jeffrey L. Ardell, Kalyanam Shivkumar bioRxiv 2020.10.28.359109.	SAN/atria preparation (human)	VSD (Di-4-ANEPPS)	MiCAM05
Fibroblast growth factor homologous factors tune arrhythmogenic late Na_V1.5 current in calmodulin binding?deficient channels. Jeffrey Abrams, Daniel Roybal, Nourdine Chakouri, Alexander N. Katchman, Richard Weinberg, Lin Yang, Bi-xing Chen, Sergey I. Zakharov, Jessica A. Hennessey, Uma Mahesh R. Avula, Johanna Diaz, Chaojian Wang, Elaine Y. Wan, Geoffrey S. Pitt, Manu Ben-Johny, and Steven O. Marx JCI Insight. 2020 Oct 2; 5(19): e141736.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
cAMP-dependent regulation of HCN4 controls the tonic entrainment process in sinoatrial node pacemaker cells. Stefanie Fenske, Konstantin Hennis, René D. Rötzer, Verena F. Brox, Elvir Becirovic, Andreas Scharr, Christian Gruner, Tilman Ziegler, Verena Mehlfeld, Jaclyn Brennan, Igor R. Efimov, Audrys G. Pauža, Markus Moser, Carsten T. Wotjak, Christian Kupatt, Rasmus Gönner, Rai Zhang, Henggui Zhang, Xiangang Zong, Martin Biel and Christian Wahl-Schott Nat Commun. 2020; 11: 5555.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM05
The phospholamban p.(Arg14del) pathogenic variant leads to cardiomyopathy with heart failure and is unresponsive to standard heart failure therapy. Tim R. Eijgenraam, Bastiaan J. Boukens, Cornelis J. Boogerd, E. Marloes Schouten, Cees W. A. van de Kolk, Nienke M. Stege, Wouter P. te Rijdt, Edgar T. Hoorntje, Paul A. van der Zwaag, Eva van Rooij, J. Peter van Tintelen, Maarten P. van den Berg, Peter van der Meer, Jolanda van der Velden, Herman H. W. Silljé & Rudolf A. de Boer Scientific Reports volume 10, Article number: 9819 (2020)	Isolated heart (mouse)	VSD (RH-237)	MiCAM05
Anti-Inflammatory and Antiarrhythmic Effects of Beta Blocker in a Rat Model of Rheumatoid Arthritis. Ting-Tse Lin, Yen-Ling Sung, Jhen-Yang Syu, Kia-Yuan Lin, Hung-Jui Hsu, Min-Tsun Liao, Yen-Bin Liu, Shien-Fong Lin J Am Heart Assoc. 2020 Sep 15;9(18):e016084.	Isolated heart (rat)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Acute Effects of Ibrutinib on Ventricular Arrhythmia in Spontaneously Hypertensive Rats. Beibei Du, Praloy Chakraborty, Mohammed Ali Azam, Stéphane Massé, Patrick F.H. Lai, Ahmed Niri BEng, Daoyuan Si, Paaladinesh Thavendiranathan, Husam Abdel-Qadir, Filio Billia, Kumaraswamy Nanthakumar JACC: CardioOncology Volume 2, Issue 4, November 2020, Pages 614-629	Isolated heart (rat)	VSD RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA
Reverse electromechanical modelling of diastolic dysfunction in spontaneous hypertensive rat after sacubitril/valsartan therapy. Yen]Ling Sung, Ting]Tse Lin, Jhen]Yang Syu, Hung]Jui Hsu, Kai]Yuan Lin, Yen]Bin Liu, Shien]Fong Lin ESC Heart Failure 2020; 7: 4040?4050	Isolated heart (rat)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
In vitro generation of functional murine heart organoids via FGF₄ and extracellular matrix. Jiyoung Lee, Akito Sutani, Rin Kaneko, Jun Takeuchi, Tetsuo Sasano, Takashi Kohda, Kensuke Ihara, Kentaro Takahashi, Masahiro Yamazoe, Tomohiro Morio , Tetsushi Furukawa, Fumitoshi Ishino Nat Commun. 2020 Sep 3;11(1):4283.	Organoid	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
The sodium channel Na_V1.5 impacts on early murine embryonic cardiac development, structure and function in a non]electrogenic manner. Gerard A. Marchal, Arie O. Verkerk, Rajiv A. Mohan, Rianne Wolswinkel, Bastiaan J. D. Boukens, and Carol Ann Remme Acta Physiol (Oxf). 2020 Oct; 230(2): e13493.	Isolated heart (mouse embryo)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Sacubitril/Valsartan Therapy Ameliorates Ventricular Tachyarrhythmia Inducibility in a Rabbit Myocardial Infarction Model. Po-Cheng Chang, Hung-Ta Wo, Hui-Ling Lee, Shien-Fong Lin, Yen Chu, Ming-Shien Wen, Chung-Chuan Chou J Card Fail. 2020 Jun;26(6):527-537.	Isolated heart (rabbit)	VSD RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Single Bolus Rosuvastatin Accelerates Calcium Uptake and Attenuates Conduction Inhomogeneity in Failing Rabbit Hearts With Regional Ischemia-Reperfusion Injury. Chung-Chuan Chou, Hui-Ling Lee, Yu-Chang Huang, Hung-Ta Wo, Ming-Shien Wen, Yen Chu, Po-Cheng Chang J Cardiovasc Pharmacol. 2020 Jan;75(1):64-74.	Isolated heart (rabbit)	VSD RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Concomitant genetic ablation of L-type Ca_v1.3 (α_1D) and T-type Ca_v3.1 (α_1G) Ca²⁺ channels disrupts heart automaticity. Matthias Baudot, Eleonora Torre, Isabelle Bidaud, Julien Louradour, Angelo G. Torrente, Lucile Fossier, Leïla Talssi, Joël Nargeot, Stéphanie Barrère-Lemaire, Pietro Mesirca, and Matteo E. Mangoni Sci Rep. 2020; 10: 18906.	SAN/atria preparation (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Mechanisms of ranolazine pretreatment in preventing ventricular tachyarrhythmias in diabetic db/db mice with acute regional ischemia-reperfusion injury. Chung-Chuan Chou, Hui-Ling Lee, Gwo-Jyh Chang, Hung-Ta Wo, Tzung-Hai Yen, Ming-Shien Wen, Yen Chu, Hao-Tien Liu, Po-Cheng Chang Sci Rep. 2020 Nov 18;10(1):20032.	Isolated heart (mouse)	VSD RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices. Sharon A George, Jaclyn A Brennan, Igor R Efimov J Vis Exp. 2020 Jun 16;(160). doi: 10.3791/60781.	Tissue slice (human)	VSD RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Stretchable Conductive Fabric for Cardiac Electrophysiology Applications. Angel Moreno, Richard D. Walton, and Jason D. Bayer ACS Appl. Bio Mater. 2020, 3, 5, 3114?3122	Isolated heart (rig)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Apelin increases atrial conduction velocity, refractoriness, and prevents inducibility of atrial fibrillation. Young M Kim, Robert Lakin, Hao Zhang, Jack Liu, Ayaaz Sachedina, Maneesh Singh, Emily Wilson, Marco Perez, Subodh Verma, Thomas Quertermous, Jeffrey Olgin, Peter H Backx, Euan A Ashley JCI Insight. 2020 Sep 3;5(17):e126525.	Isolated atria (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Mechanical stimulation of endocardial Purkinje fibres can trigger ventricular arrhythmias. Ed White, Richard Walton, Sarbjot Kaur, Amelia Power, Michel Haïssaguerre, Olivier Bernus, Marie-Louise Ward bioRxiv 2020.08.10.234195	Isolated heart (rat)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Attenuating loss of cardiac conduction during no-flow ischemia through changes in perfusate sodium and calcium. Gregory S Hoeker, Carissa C James, Allison N Tegge, Robert G Gourdie, James W Smyth, Steven Poelzing Am J Physiol Heart Circ Physiol. 2020 Aug 1;319(2):H396-H409.	Isolated heart (guinea pig)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
ZO-1 Regulates Intercalated Disc Composition and Atrioventricular Node Conduction. Wenli Dai, Rangarajan D. Nadadur, Jaclyn A. Brennan, Heather L. Smith, Kaitlyn M. Shen, Margaret Gadek, Brigitte Laforest, Mingyi Wang, Joanna Gemel, Ye Li, Jing Zhang, Bruce D. Ziman, Jiajie Yan, Xun Ai, Eric C. Beyer, Edward G. Lakata, Narayanan Kasthuri, Igor R. Efimov, Michael T. Broman, Ivan P. Moskowitz, Le Shen, Christopher R. Weber Circulation Research. 2020;127:e28?e43	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Effects of high-frequency biphasic shocks on ventricular vulnerability and defibrillation outcomes through synchronized virtual electrode responses. Yu-An Chiou, Li-Kuan Cheng, Shien-Fong Lin PLoS One. 2020; 15(5): e0232529.	Isolated heart (rat)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Connexin43 expression in bone marrow derived cells contributes to the electrophysiological properties of cardiac scar tissue. Carolina Vasquez, Valeria Mezzano, Newman Kessler, Freja Swardh, Desiree Ernestad, Vanessa M. Mahoney, John Hanna & Gregory E. Morley Scientific Reports volume 10, Article number: 2617 (2020)	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Vascular endothelial growth factor promotes atrial arrhythmias by inducing acute intercalated disk remodeling. Louisa Mezache, Heather L. Struckman, Amara Greer-Short, Stephen Baine, Sándor Györke, Przemysław B. Radwański, Thomas J. Hund and Rengasayee Veeraraghavan Sci Rep. 2020; 10: 20463.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Adrenergic supersensitivity and impaired neural control of cardiac electrophysiology following regional cardiac sympathetic nerve loss. Srinivas Tapa, Lianguo Wang, Samantha D. Francis Stuart, Zhen Wang, Yanyan Jiang, Beth A. Habecker & Crystal M. Ripplinger Scientific Reports volume 10, Article number: 18801 (2020)	Isolated heart (mouse)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Aging Disrupts Normal Time-of-Day Variation in Cardiac Electrophysiology. Zhen Wang, Srinivas Tapa, Samantha D Francis Stuart, Lianguo Wang, Julie Bossuyt, Brian P Delisle, Crystal M Ripplinger Circ Arrhythm Electrophysiol. 2020 Sep;13(9):e008093.	Isolated heart (mouse)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Optical Mapping-Validated Machine Learning Improves Atrial Fibrillation Driver Detection by Multi-Electrode Mapping. Alexander M Zolotarev, Brian J Hansen, Ekaterina A Ivanova, Katelynn M Helfrich, Ning Li, Paul M L Janssen, Peter J Mohler, Nahush A Mokadam, Bryan A Whitson, Maxim V Fedorov, John D Hummel, Dmitry V Dylov, Vadim V Fedorov Circ Arrhythm Electrophysiol. 2020 Oct;13(10):e008249.	Isolated atria (human)	VSD (Di-4-ANBDQBS)	MiCAM ULTIMA
Cold-inducible RNA-binding protein modulates atrial fibrillation onset by targeting multiple ion channels. Duanyang Xie, Li Geng, Shuo Wang, Ke Xiong, Tingting Zhao, Guanghua Wang, Zhiqiang Feng, Fei Lv, Cheng Wang, Dandan Liang, Dan ShiMD, Xiue MaMD, Yi Liu, Jian Yang, Chao Zhang, Yi-Han Chen Heart Rhythm Volume 17, Issue 6, June 2020, Pages 998-1008	Isolated heart (rat)	VSD (RH-237)	MiCAM ULTIMA
What Is the Optimal Light Source for Optical Mapping Using Voltage- and Calcium-Sensitive Dyes? V Olejnickova, D Sedmera Physiol Res. 2020 Aug 31;69(4):599-607	Isolated heart (chick embryo)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Cardiac sympathetic nerve transdifferentiation reduces action potential heterogeneity after myocardial infarction. Lianguo Wang, Antoinette Olivas, Samantha D Francis Stuart, Srinivas Tapa, Matthew R Blake, William R Woodward, Beth A Habecker, Crystal M Ripplinger Am J Physiol Heart Circ Physiol. 2020 Mar 1;318(3):H558-H565.	Isolated heart (mouse)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Evidence of Superior and Inferior Sinoatrial Nodes in the Mammalian Heart. Jaclyn A. Brennan, Qing Chen, Anna Gams, Jhansi Dyavanapalli, David Mendelowitz, Weiqun Peng, Igor R.Efimov JACC Clin Electrophysiol. 2020 Dec;6(14):1827-1840.	Isolated atrium (human)	VSD (Di-4-ANEPPS)	MiCAM05
Sudden Heart Rate Reduction Upon Optogenetic Release of Acetylcholine From Cardiac Parasympathetic Neurons in Perfused Hearts. Angel Moreno, Kendal Endicott, Matthew Skancke, Mary Kate Dwyer, Jaclyn Brennan, Igor R. Efimov, Gregory Trachiotis, David Mendelowitz, and Matthew W. Kay Front Physiol. 2019 Sep 4;10:1105.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Acetylcholine Delays Atrial Activation to Facilitate Atrial Fibrillation. Jason D. Bayer, Bastiaan J. Boukens, Sébastien P. J. Krul, Caroline H. Roney, Antoine H. G. Driessen, Wouter R. Berger, Nicoline W. E. van den Berg, Arie O. Verkerk, Edward J. Vigmond, Ruben Coronel, and Joris R. de Groot Front Physiol. 2019 Sep 4;10:1105.	Atrial appendage preparation (human)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Caveolae]Mediated Activation of Mechanosensitive Chloride Channels in Pulmonary Veins Triggers Atrial Arrhythmogenesis. Yuriy V. Egorov, Di Lang, Leonid Tyan, Daniel Turner, Evi Lim, Zachary D. Piro, Jonathan J. Hernandez, Rylie Lodin, Rose Wang, Eric G. Schmuck, Amish N. Raval, Carter J. Ralphe, Timothy J. Kamp, Leonid V. Rosenshtraukh, and Alexey V. Glukhov Sci Rep. 2019; 9: 7502.	Pulmonary vein preparation (rat)	VSD (RH-237)	MiCAM ULTIMA
Atrial fibrillation and electrophysiology in transgenic mice with cardiac-restricted overexpression of FKBP12. Zhenwei Pan, Tomohiko Ai, Po-Cheng Chang, Ying Liu, Jijia Liu, Mitsunori Maruyama, Mohamed Homsi, Michael C. Fishbein, Michael Rubart, Shien-Fong Lin, Deyong Xiao, Hanying Chen, Peng-Sheng Chen, Weinian Shou and Bai-Yan Li Am J Physiol Heart Circ Physiol. 2019 Feb 1; 316(2): H371?H379.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Inhibition of PI3Kinase-α is pro-arrhythmic and associated with enhanced late Na⁺ current, contractility, and Ca²⁺ release in murine hearts. Pavel Zhabyeyev, Brent McLean, Xueyi Chen, Bart Vanhaesebroeck, Gavin Y. Oudit Theranostics. 2019; 9(24): 7222?7238.	Isolated heart (mouse)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Cardiac overexpression of perilipin 2 induces atrial steatosis, connexin 43 remodeling, and atrial fibrillation in aged mice. Satsuki Sato, Jinya Suzuki, Masamichi Hirose, Mika Yamada, Yasuo Zenimaru, Takahiro Nakaya, Mai Ichikawa, Michiko Imagawa, Sadao Takahashi, Shoichiro Ikuyama, Tadashi Konoshita, Fredric B Kraemer, Tamotsu Ishizuka Am J Physiol Endocrinol Metab. 2019 Dec 1;317(6):E1193-E1204.	Isolated heart (mouse)	VSD (RH-237)	MiCAM02
Short-Long Heart Rate Variation Increases Dispersion of Action Potential Duration in Long QT Type 2 Transgenic Rabbit Model. Tae Yun Kim, Paul Jeng, JungMin Hwang, Zachary Pfeiffer, Divyang Patel, Leroy L Cooper, Konstantinos Kossidas, Jason Centracchio, Xuwen Peng, Gideon Koren, Zhilin Qu, Bum-Rak Choi Sci Rep. 2019 Oct 16;9(1):14849.	Isolated heart (rabbit)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
PKA phosphorylation underlies functional recruitment of sarcolemmal SK2 channels in ventricular myocytes from hypertrophic hearts. Shanna Hamilton, Iuliia Polina, Radmila Terentyeva, Peter Bronk, Tae Yun Kim, Karim Roder, Richard T Clements, Gideon Koren, Bum-Rak Choi, Dmitry Terentyev J Physiol. 2020 Jul;598(14):2847-2873.	Isolated heart (rat)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Variation in a Left Ventricle-Specific Hand1 Enhancer Impairs GATA Transcription Factor Binding and Disrupts Conduction System Development and Function. Joshua W Vincentz, Beth A Firulli, Kevin P Toolan, Dan E Arking, Nona Sotoodehnia, Juyi Wan, Peng-Sheng Chen, Corrie de Gier-de Vries, Vincent M Christoffels, Michael Rubart-von der Lohe, Anthony B Firulli Circ Res. 2019 Aug 30;125(6):575-589.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Absence of natriuretic peptide clearance receptor attenuates TGF-β1-induced selective atrial fibrosis and atrial fibrillation. Dolkun Rahmutula, Hao Zhang, Emily E Wilson, Jeffrey E Olgin Cardiovasc Res. 2019 Feb 1;115(2):357-372.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Relaxin reverses maladaptive remodeling of the aged heart through Wnt-signaling. Brian Martin, Beth Gabris, Amr F. Barakat, Brian L. Henry, Marianna Giannini, Rajiv P. Reddy, Xuewen Wang, Guillermo Romero & Guy Salama Scientific Reports volume 9, Article number: 18545 (2019)	Isolated heart (rat)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Heterogeneity of the action potential duration is required for sustained atrial fibrillation. Uma Mahesh R. Avula, Jeffrey Abrams, Alexander Katchman, Sergey Zakharov, Sergey Mironov, Joseph Bayne, Daniel Roybal, Anirudh Gorti, Lin Yang, Vivek Iyer, Marc Waase, Deepak Saluja, Edward J. Ciaccio, Hasan Garan, Andrew R. Marks, Steven O. Marx, and Elaine Y. Wan JCI Insight. 2019 Apr 25;5(11):e128765.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Functional Consequences of the SCN5A-p.Y1977N Mutation within the PY Ubiquitylation Motif: Discrepancy between HEK293 Cells and Transgenic Mice. Simona Casini, Maxime Albesa, Zizun Wang, Vincent Portero, Daniela Ross-Kaschitza, Jean-Sébastien Rougier, Gerard A. Marchal, Wendy K. Chung, Connie R. Bezzina, Hugues Abriel, and Carol Ann Remme Int J Mol Sci. 2019 Oct; 20(20): 5033.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Wide-area low-energy surface stimulation of large mammalian ventricular tissue. Angel Moreno, Richard D Walton, Marion Constantin, Olivier Bernus, Edward J Vigmond, Jason D Bayer Front Physiol. 2019 Feb 26;10:146.	Ventricular wedge preparation (pig)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Optical Imaging of Ventricular Action Potentials in a Torso Tank: A New Platform for Non-Invasive Electrocardiographic Imaging Validation. Laura R Bear, Richard D Walton, Emma Abell, Yves Coudière, Michel Haissaguerre, Olivier Bernus, Rémi Dubois Front Physiol. 2019 Feb 26;10:146.	Isolated heart (pig)	VSD (Di-4-ANBDQBS)	MiCAM ULTIMA
Wireless, battery-free, fully implantable multimodal and multisite pacemakers for applications in small animal models. Philipp Gutruf, Rose T Yin, K Benjamin Lee, Jokubas Ausra, Jaclyn A Brennan, Yun Qiao, Zhaoqian Xie, Roberto Peralta, Olivia Talarico, Alejandro Murillo, Sheena W Chen, John P Leshock, Chad R Haney, Emily A Waters, Changxing Zhang, Haiwen Luan, Yonggang Huang, Gregory Trachiotis, Igor R Efimov, John A Rogers Nat Commun. 2019 Dec 17;10(1):5742.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Simplified platform for mosaic in vivo analysis of cellular maturation in the developing heart. Julie Goudy, Trevor Henley, Hernán G. Méndez and Michael Bressan Scientific Reports volume 9, Article number: 10716 (2019)	Isolated heart (chick embryo)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Inhibition of soluble TNFα prevents adverse atrial remodeling and atrial arrhythmia susceptibility induced in mice by endurance exercise. Robert Lakin, Nazari Polidovitch, Sibao Yang, Camilo Guzman, Xiaodong Gao, Marianne Wauchop, Jacob Burns, Farzad Izaddoustdar, Peter H Backx J Mol Cell Cardiol. 2019 Apr;129:165-173.	Isolated atria (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Different paths, same destination: divergent action potential responses produce conserved cardiac fight-or-flight response in mouse and rabbit hearts. Lianguo Wang, Stefano Morotti, Srinivas Tapa, Samantha D Francis Stuart, Yanyan Jiang, Zhen Wang, Rachel C Myles, Kieran E Brack, G André Ng, Donald M Bers, Eleonora Grandi, Crystal M Ripplinger J Physiol. 2019 Aug;597(15):3867-3883.	Isolated heart (rabbit)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
YAP Partially Reprograms Chromatin Accessibility to Directly Induce Adult Cardiogenesis In Vivo. Tanner O Monroe, Matthew C Hill, Yuka Morikawa, John P Leach, Todd Heallen, Shuyi Cao, Peter H L Krijger, Wouter de Laat, Xander H T Wehrens, George G Rodney, James F Martin Dev Cell. 2019 Mar 25;48(6):765-779.e7.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Exposure to Secondhand Smoke and Arrhythmogenic Cardiac Alternans in a Mouse Model. Zhen Wang, Lianguo Wang, Srinivas Tapa, Kent E. Pinkerton, Chao-Yin Chen and Crystal M. Ripplinger Environmental Health Perspectives. 126(12) December 2018	Isolated heart (mouse)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Ventricular Endocardial Tissue Geometry Affects Stimulus Threshold and Effective Refractory Period. Adam Connolly, Allen Kelly, Fernando O. Campos, Rachel Myles, Godfrey Smith and Martin J. Bishop Biophys J. 2018 Dec 18;115(12):2486-2498.	Ventricular wedge preparation (rabbit)	VSD (RH-237)	MiCAM ULTIMA
Critical Volume of Human Myocardium Necessary to Maintain Ventricular Fibrillation. Kedar K. Aras, Ndeye Rokhaya Faye, Brianna Cathey and Igor R. Efimov Circ Arrhythm Electrophysiol. 2018 Nov;11(11):e006692.	Ventricular wedge preparation (human)	VSD (Di-4-ANBDQBS)	MiCAM05 (quad camera)
Arrhythmogenic Remodeling of the Left Ventricle in a Porcine Model of Repaired Tetralogy of Fallot. Virginie Dubes, David Benoist, François Roubertie, Stephen H. Gilbert, Marion Constantin, Sabine Charron, Delphine Elbes, Delphine Vieillot, Bruno Quesson, Hubert Cochet, Michel Haïssaguerre, Caroline Rooryck, Pierre Bordachar, Jean-Benoit Thambo, and Olivier Bernus Circ Arrhythm Electrophysiol. 2018;11:e006059.	Ventricular wedge preparation (pig)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Long non-coding RNA CCRR controls cardiac conduction via regulating intercellular coupling. Yong Zhang, Lihua Sun, Lina Xuan, Zhenwei Pan, Xueling Hu, Hongyu Liu, Yunlong Bai, Lei Jiao, Zhange Li, Lina Cui, Xiaoxue Wang, Siqi Wang, Tingting Yu, Bingbing Feng, Ying Guo, Zonghong Liu, Weixin Meng, Hequn Ren, Jiyuan Zhu, Xuyun Zhao, Chao Yang, Ying Zhang, Chaoqian Xu, Zhiguo Wang, Yanjie Lu, Hongli Shan, and Baofeng Yang Nat Commun. 2018 Oct 9;9(1):4176.	Isolated heart (mouse)	VSD (RH-237)	MiCAM05
CRISPR]Mediated Expression of the Fetal Scn5a Isoform in Adult Mice Causes Conduction Defects and Arrhythmias. Paul D. Pang, Katherina M. Alsina, Shuyi Cao, Amrita B. Koushik, Xander H.T. Wehrens and Thomas A. Cooper J Am Heart Assoc. 2018;7:e010393.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
HuR-mediated SCN5A messenger RNA stability reduces arrhythmic risk in heart failure. Anyu Zhou, An Xie, Tae Yun Kim, Hong Liu, Guangbin Shi, Gyeoung-Jin Kang, Ning Jiang, Man Liu, Euy-Myoung Jeong, Bum-Rak Choi, and Samuel C. Dudley Jr. Heart Rhythm. 2018 Jul;15(7):1072-1080.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Role of the Purkinje-Muscle Junction on the Ventricular Repolarization Heterogeneity in the Healthy and Ischemic Ovine Ventricular Myocardium. Marine E. Martinez, Richard D. Walton, Jason D. Bayer, Michel Haïssaguerre, Edward J. Vigmond, Mélèze Hocini and Olivier Bernus Front Physiol. 2018 Jun 14;9:718.	Ventricular wedge preparation (sheep)	VSD (Di-4-ANBDQBS)	MiCAM ULTIMA
Small-conductance Ca₂₊-activated K₊ channel activation deteriorates hypoxic ventricular arrhythmias via CaMKII in cardiac hypertrophy. Taro Tenma, Hirofumi Mitsuyama, Masaya Watanabe, Naoya Kakutani, Yutaro Otsuka, Kazuya Mizukami, Rui Kamada, Masayuki Takahashi, Shingo Takada, Hisataka Sabe, Hiroyuki Tsutsui, and Hisashi Yokoshiki Am J Physiol Heart Circ Physiol. 2018 Aug 1;315(2):H262-H272.	Isolated heart (rat)	VSD (Di-4-ANEPPS)	MiCAM02
Absence of NPR-C Attenuates TGF-s1 Induced Selective Atrial Fibrosis and Atrial Fibrillation. Dolkun Rahmutula, Hao Zhang, Emily E Wilson and Jeffrey E Olgin Cardiovasc Res. 2018 Sep 18.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Essential Role of RyR2 Phosphorylation in the Effect of Azumolene on Ventricular Arrhythmia Vulnerability in a Rabbit Heart Model. Daoyuan Si, Mohammed Ali Azam, Patrick F.H. Lai, Nima Zamiri, Galina Kichigina, John Asta, Stéphane Massé, Mahmoud M. Bokhari, Andreu Porta]Sánchez, Christopher Labos, Huan Sun, Ping Yang and Kaumaraswamy Nanthakumar J Cardiovasc Electrophysiol. 2018 Sep 11.	Isolated heart (rabbit)	CaSD (Rhod-2 AM)	MiCAM ULTIMA
Apoptosis and epicardial contributions act as complementary factors in remodeling of the atrioventricular canal myocardium and atrioventricular conduction patterns in the embryonic chick heart. Rebecca Vicente Steijn, David Sedmera, Nico A. Blom, Monique Jongbloed, Alena Kvasilova, and Ondrej Nanka Dev Dyn. 2018 Sep;247(9):1033-1042.	Isolated heart (chick embryo)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Effects of long-term exercise on arrhythmogenesis in aged hypertensive rats. Yen-Ling Sung, Chih-En Wu, Jhen-Yang Syu,Terry B.J.Kuo, Jai-YiLi, Chieh-Wen Chen, Ching-Hui Weng, Wei-Hsuan Hsu, Shih-Ann Chen, Yu-Feng Hu and Shien-Fong Lin Comput Biol Med. 2018 Aug 16. pii: S0010-4825(18)30236-1.	Isolated heart (rat)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Compartmentalized Structure of the Moderator Band Provides a Unique Substrate for Macroreentrant Ventricular Tachycardia. Richard D. Walton, Ali Pashaei, Marine E. Martinez, Marion Constantin, Josselin Duchateau, Laura Bear, Caroline Cros, Caroline Pascarel-Auclerc, Yunbo Guo, David Benoist, Virginie Dubes, Ndeye Rokhaya Faye, Sebastien Chaigne, Sebastien Dupuis, Dominique Détaille, Line Pourtau, Philippe Pasdois, Fabien Brette, Julien Rogier, Louis Labrousse, Mélèze Hocini, Edward J. Vigmond, Michel Haïssaguerre and Olivier Bernus Circulation: Arrhythmia and Electrophysiology. 2018;11:e005913	Ventricular wedge preparation (sheep, human)	VSD (Di]4]ANBDQBS)	MiCAM ULTIMA
CaMKII-dependent late Na⁺ current increases electrical dispersion and arrhythmia in ischemia-reperfusion. Taylor Howard, Amara Greer-Short, Tony Satroplus, Nehal Patel, Drew Nassal, Peter J. Mohler, and Thomas J. Hund Am J Physiol Heart Circ Physiol. 2018 Oct 1;315(4):H794-H801.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM05
LncRNA ZFAS1 as a SERCA2a Inhibitor to Cause Intracellular Ca²⁺ Overload and Contractile Dysfunction in a Mouse Model of Myocardial Infarction. Ying Zhang, Lei Jiao, Lihua Sun, Yanru Li, Yuqiu Gao, Chaoqian Xu, Yingchun Shao, Mengmeng Li, Chunyan Li, Yanjie Lu, Zhenwei Pan, Lina Xuan, Yiyuan Zhang, Qingqi Li, Rui Yang, Yuting Zhuang, Yong Zhang and Baofeng Yang Circ Res. 2018 May 11; 122(10): 1354?1368.	Isolated heart (mouse)	CaSD (Rhod-2 AM)	MiCAM05
Age-related changes in cardiac electrophysiology and calcium handling in response to sympathetic nerve stimulation. Samantha D. Francis Stuart, Lianguo Wang, William R. Woodard, G. Andre Ng, Beth A. Habecker and Crystal M. Ripplinger J Physiol. 2018 Jun 25.	Isolated heart (mouse)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Transient denervation of viable myocardium after myocardial infarction does not alter arrhythmia susceptibility. Diana C. Parrish, Samantha D. Francis Stuart, Antoinette Olivas, Lianguo Wang, Anders Nykjaer, Crystal M. Ripplinger, and Beth A. Habecker Am J Physiol Heart Circ Physiol. 2018 Mar 1;314(3):H415-H423.	Isolated heart (mouse)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
HuR-mediated SCN5A messenger RNA stability reduces arrhythmic risk in heart failure. Anyu Zhou, An Xie, Tae Yun Kim, Hong Liu, Guangbin Shi, Gyeoung-Jin Kang, Ning Jiang, Man Liu, Euy-Myoung Jeong, Bum-Rak Choi, Samuel C. Dudley Jr. Heart Rhythm. 2018 Feb 16.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Oxidized CaMKII (Ca²⁺/Calmodulin-Dependent Protein Kinase II) Is Essential for Ventricular Arrhythmia in a Mouse Model of Duchenne Muscular Dystrophy. Qiongling Wang, Ann P. Quick, Shuyi Cao, Julia Reynolds, David Y. Chiang, David Beavers, Na Li, Guoliang Wang, George G. Rodney, Mark E. Anderson, Xander H.T. Wehrens. Circ Arrhythm Electrophysiol. 2018 Apr;11(4):e005682.	Isolated heart (mouse)	VSD (RH-237)	MiCAM ULTIMA
Ondansetron Blocks Wildtype and p.F503L Variant Small Conductance Calcium Activated Potassium Channels. Jum-Suk Ko, Shuai Guo, Jonathan Hassel, Patricia Celestino-Soper, Ty C Lynnes, James E Tisdale, James J Zheng, Stanley E Taylor, Tatiana Foroud, Michael D Murray, Richard J Kovacs, Xiaochun Li, Shien-Fong Lin, Zhenhui Chen, Matteo Vatta, Peng-Sheng Chen, and Michael Rubart Am J Physiol Heart Circ Physiol. 2018 Apr 20.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Overexpression of Cx43 in cells of the myocardial scar: Correction of post-infarct arrhythmias through heterotypic cell-cell coupling. Wilhelm Roell, Alexandra M. Klein, Martin Breitbach, Torsten S. Becker, Ashish Parikh, Jane Lee, Katrin Zimmermann, Shaun Reining, Beth Gabris, Annika Ottersbach, Robert Doran, Britta Engelbrecht, Miriam Schiffer, Kenichi Kimura, Patricia Freitag, Esther Carls, Caroline Geisen, Georg D. Duerr, Philipp Sasse, Armin Welz, Alexander Pfeifer, Guy Salama, Michael Kotlikoff & Bernd K. Fleischmann Sci Rep. 2018 May 8;8(1):7145.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Electrophysiologic effects of the IK1 inhibitor PA-6 are modulated by extracellular potassium in isolated guinea pig hearts. Gregory S. Hoeker, Mark A. Skarsfeldt, Thomas Jespersen, and Steven Poelzing Physiol Rep. 2017 Jan;5(1). pii: e13120. doi: 10.14814/phy2.13120. Epub 2017 Jan 13.	Isolated heart (guinea pig)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Redundant and diverse intranodal pacemakers and conduction pathways protect the human sinoatrial node from failure. Ning Li, Brian J. Hansen, Thomas A. Csepe, Jichao Zhao, Anthony J. Ignozzi, Lidiya V. Sul, Stanislav O. Zakharkin, Anuradha Kalyanasundaram, Jonathan P. Davis, Brandon J. Biesiadecki, Ahmet Kilic, Paul M. L. Janssen, Peter J. Mohler, Raul Weiss, John D. Hummel and Vadim V. Fedorov Sci Transl Med. 2017 Jul 26;9(400).	SAN preparation (human)	VSD (Di]4]ANBDQBS)	MiCAM ULTIMA (dual or quad camera)
Three-dimensional Integrated Functional, Structural, and Computational Mapping to Define the Structural "Fingerprints" of Heart-Specific Atrial Fibrillation Drivers in Human Heart Ex Vivo. Jichao Zhao, Brian J. Hansen, Yufeng Wang, Thomas A. Csepe, Lidiya V. Sul, Alan Tang, Yiming Yuan, Ning Li, Anna Bratasz, Kimerly A. Powell, Ahmet Kilic, Peter J. Mohler, Paul M. L. Janssen, Raul Weiss, Orlando P. Simonetti, John D. Hummel, and Vadim V. Fedorov J Am Heart Assoc. 2017 Aug 22;6(8).	Atrial tissue (human)	VSD (Di]4]ANBDQBS)	MiCAM ULTIMA
Molecular Mechanisms and New Treatment Paradigm for Atrial Fibrillation. Padmini Sirish, Ning Li, Valeriy Timofeyev, Xiao-Dong Zhang, Lianguo Wang, Jun Yang, Kin Sing S. Lee, Ahmed Bettaieb, Sin Mei Ma, Jeong Han Lee, Demetria Su, Victor C. Lau, Richard E. Myers, Deborah K. Lieu, Javier E. López, J. Nilas Young, Ebenezer N. Yamoah, Fawaz Haj, Crystal M. Ripplinger, Bruce D. Hammock, and Nipavan Chiamvimonvat Circ Arrhythm Electrophysiol. 2016 May; 9(5)	Isolated heart (mouse)	VSD (RH-237)	MiCAM ULTIMA
Anti-arrhythmic effects of interleukin-1 inhibition following myocardial infarction. Nicole M. De Jesus, Lianguo Wang, Johnny Lai, Robert R. Rigor, Samantha D. Francis Stuart, Donald M. Bers, Merry L. Lindsey, and Crystal M. Ripplinger Heart Rhythm. 2017 May; 14(5): 727?736.	Isolated heart (mouse)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
β-Adrenergic Inhibition Prevents Action Potential and Calcium Handling Changes during Regional Myocardial Ischemia. Shannon R. Murphy, Lianguo Wang, Zhen Wang, Philip Domondon, Di Lang,1 Beth A. Habecker, Rachel C. Myles, and Crystal M. Ripplinger Front Physiol. 2017 Aug 28;8:630.	Isolated heart (rabbit)	VSD (RH-237) CaSD (Fluo-5N AM)	MiCAM ULTIMA (dual camera)
β-adrenergic stimulation augments transmural dispersion of repolarization via modulation of delayed rectifier currents IKs and IKr in the human ventricle. C. Kang, A. Badiceanu, J. A. Brennan, C. Gloschat, Y. Qiao, N. A. Trayanova, and I. R. Efimov Sci Rep. 2017 Nov 21;7(1):15922.	Ventricular wedge preparation (human)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Human Organotypic Cultured Cardiac Slices: New Platform For High Throughput Preclinical Human Trials. C. Kang, Y. Qiao, G. Li, K. Baechle, P. Camelliti, S. Rentschler, and I. R. Efimov Sci Rep. 2016; 6: 28798.	Ventricular slice (human)	VSD (Di-4-ANEPPS and RH-237) CaSD (Rhod-2)	MiCAM ULTIMA
Connexin43 contributes to electrotonic conduction across scar tissue in the intact heart. Vanessa M. Mahoney, Valeria Mezzano, Gary R. Mirams, Karen Maass, Zhen Li, Marina Cerrone, Carolina Vasquez, Aneesh Bapat, Mario Delmar, and Gregory E. Morley Sci Rep. 2016 May 31;6:26744. doi: 10.1038/srep26744.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Optogenetic termination of ventricular arrhythmias in the whole heart: towards biological cardiac rhythm management. Emile C.A. Nyns, Annemarie Kip, Cindy I. Bart, Jaap J. Plomp; Katja Zeppenfeld, Martin J. Schalij, Antoine A.F. de Vries, Daniël A. Pijnappels Eur Heart J. 2016 Dec 23. pii: ehw574. doi: 10.1093/eurheartj/ehw574. [Epub ahead of print]	Isolated heart (rat)	VSD (Di-4-ANBDQBS)	MiCAM ULTIMA
Resolving Myocardial Activation With Novel Omnipolar Electrograms. Stéphane Massé, Karl Magtibay, Nicholas Jackson, John Asta, Marjan Kusha, Boyang Zhang, Ram Balachandran, Milica Radisic, D. Curtis Deno, and Kumaraswamy Nanthakumar Circ Arrhythm Electrophysiol. 2016 Jul;9(7):e004107. doi: 10.1161/CIRCEP.116.004107.	Isolated heart (rabbit)	VSD (Di-4-ANBDQPQ)	MiCAM ULTIMA
Aberrant sodium influx causes cardiomyopathy and atrial fibrillation in mice. Elaine Wan, Jeffrey Abrams, Richard L. Weinberg, Alexander N. Katchman, Joseph Bayne, Sergey I. Zakharov, Lin Yang, John P. Morrow, Hasan Garan, and Steven O. Marx J Clin Invest. 2016 Jan;126(1):112-22. doi: 10.1172/JCI84669. Epub 2015 Nov 23.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Aberrant sodium influx causes cardiomyopathy and atrial fibrillation in mice. Elaine Wan, Jeffrey Abrams, Richard L. Weinberg, Alexander N. Katchman, Joseph Bayne, Sergey I. Zakharov, Lin Yang, John P. Morrow, Hasan Garan, and Steven O. Marx J Clin Invest. 2016 Jan;126(1):112-22. doi: 10.1172/JCI84669. Epub 2015 Nov 23.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Extracellular sodium dependence of the conduction velocity-calcium relationship: evidence of ephaptic self-attenuation. Sharon A. George, Mohammad Bonakdar, Michael Zeitz, Rafael V. Davalos, James W. Smyth, Steven Poelzing Am J Physiol Heart Circ Physiol. 2016 May 1;310(9):H1129-39. doi: 10.1152/ajpheart.00857.2015. Epub 2016 Mar 4.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Heart Rate and Extracellular Sodium and Potassium Modulation of Gap Junction Mediated Conduction in Guinea Pigs. Michael Entz, II, Sharon A. George, Michael J. Zeitz, Tristan Raisch, James W. Smyth, and Steven Poelzing Front Physiol. 2016 Feb 2;7:16. doi: 10.3389/fphys.2016.00016. eCollection 2016.	Isolated heart (guinea pig)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Adenosine-Induced Atrial Fibrillation: Localized Reentrant Drivers in Lateral Right Atria due to Heterogeneous Expression of Adenosine A1 Receptors and GIRK4 Subunits in the Human Heart. Ning Li, Thomas A. Csepe, Brian J. Hansen, Lidiya V. Sul, Anuradha Kalyanasundaram, Stanislav O. Zakharkin, Jichao Zhao, Avirup Guha, David R. Van Wagoner, Ahmet Kilic, Peter J. Mohler, Paul M. L. Janssen, Brandon J. Biesiadecki, John D. Hummel, Raul Weiss and Vadim V. Fedorov Circulation. 2016 Aug 9;134(6):486-98. doi: 10.1161/CIRCULATIONAHA.115.021165. Epub 2016 Jul 26.	Atria tissue (human)	VSD (Di-4-ANBDQBS)	MiCAM ULTIMA (dual or quad camera)
Spontaneous initiation of premature ventricular complexes and arrhythmias in type 2 long QT syndrome. Xiaodong Huang, Tae Yun Kim, Gideon Koren, Bum-Rak Choi and Zhilin Qu Am J Physiol Heart Circ Physiol. 2016 Oct 7:ajpheart.00500.2016. doi: 10.1152/ajpheart.00500.2016. [Epub ahead of print]	Isolated heart (rabbit)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Fhf2 gene deletion causes temperature-sensitive cardiac conduction failure. David S. Park, Akshay Shekhar, Christopher Marra, Xianming Lin, Carolina Vasquez, Sergio Solinas, Kevin Kelley, Gregory Morley, Mitchell Goldfarb, and Glenn I. Fishman Nat Commun. 2016 Oct 4;7:12966. doi: 10.1038/ncomms12966.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Potassium channels in the Cx43 gap junction perinexus modulate ephaptic coupling: an experimental and modeling study. Rengasayee Veeraraghavan, Joyce Lin, James P. Keener, Robert Gourdie and Steven Poelzing Pflugers Arch. 2016 Oct;468(10):1651-61. doi: 10.1007/s00424-016-1861-2. Epub 2016 Aug 11.	Isolated heart (guinea pig)	VSD (Di-4-ANEPPS)	MiCAM02
Acute temperature effects on function of the chick embryonic heart. Vostarek F, Svatunkova J, Sedmera D Acta Physiol (Oxf). 2016 Aug;217(4):276-86. doi: 10.1111/apha.12691. Epub 2016 May 12.	Isolated heart (chick embryo)	VSD (Di-4-ANEPPS) CaSD (Rhod-2)	MiCAM ULTIMA
Increased susceptibility of spontaneously hypertensive rats to ventricular tachyarrhythmias in early hypertension. Thao P. Nguyen, Ali A. Sovari, Arash Pezhouman, Shankar Iyer, Hong Cao, Christopher Y. Ko, Aneesh Bapat, Nooshin Vahdani, Mostafa Ghanim, Michael C. Fishbein, and Hrayr S. Karagueuzian J Physiol. 2016 Mar 15;594(6):1689-707. doi: 10.1113/JP271318. Epub 2016 Jan 18.	Isolated heart (rat)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Molecular Basis of Hypokalemia-Induced Ventricular Fibrillation. Arash Pezhouman, Neha Singh, Zhen Song, Michael Nivala, Anahita Eskandari, Hong Cao, Aneesh Bapat, Christopher Y. Ko, Thao Nguyen, Zhilin Qu, Hrayr S. Karagueuzian and James N. Weiss Circulation. 2015 Oct 20;132(16):1528-37. doi: 10.1161/CIRCULATIONAHA.115.016217. Epub 2015 Aug 12.	Isolated heart (rabbit)	VSD (RH-237)	MiCAM ULTIMA
Myocardial Infarction Causes Transient Cholinergic Transdifferentiation of Cardiac Sympathetic Nerves via gp130. Antoinette Olivas, Ryan T. Gardner, Lianguo Wang, Crystal M. Ripplinger, William R. Woodward and Beth A. Habecker J Neurosci. 2016 Jan 13;36(2):479-88. doi: 10.1523/JNEUROSCI.3556-15.2016.	Isolated heart (rabbit)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Deletion of the Serotonin Receptor Type 3A in Mice Leads to Sudden Cardiac Death During Pregnancy. Hyewon Park, Chang-Myung Oh, Junbeom Park, Hyelim Park, Shanyu Cui, Hyung Suk Kim, Jun Namkung, Sang-kyu Park, Hui-Nam Pak, Moon-Hyoung Lee, Hail Kim, and Boyoung Joung Circ J. 2015;79(8):1807-15. doi: 10.1253/circj.CJ-14-1074. Epub 2015 May 15.	Isolated heart (mouse)	VSD (RH-237)	MiCAM ULTIMA
Extracellular sodium and potassium levels modulate cardiac conduction in mice heterozygous null for the Connexin43 gene. Sharon A George, Katherine J. Sciuto, Joyce Lin, Mohamed E Salama, James P Keener, Robert G Gourdie, and Steven Poelzing Pflugers Arch. 2015 Nov;467(11):2287-97. doi: 10.1007/s00424-015-1698-0. Epub 2015 Mar 14.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Human sinoatrial node structure: 3D microanatomy of sinoatrial conduction pathways. Thomas A. Csepe, Jichao Zhao, Brian J. Hansen, Ning Li, Lidiya V. Sul, Praise Lim, Yufeng Wang, Orlando P. Simonetti, Ahmet Kilic, Peter J. Mohler, Paul M.L. Janssen, Vadim V. Fedorov Prog Biophys Mol Biol. 2015 Dec 30. pii: S0079-6107(15)00260-6. doi: 10.1016/j.pbiomolbio.2015.12.011. [Epub ahead of print]	SAN tissue	VSD (Di-4-ANBDQBS)	MiCAM ULTIMA
Integration of High-Resolution Optical Mapping and 3-Dimensional Micro-Computed Tomographic Imaging to Resolve the Structural Basis of Atrial Conduction in the Human Heart. Jichao Zhao, Brian J. Hansen, Thomas A. Csepe, Praise Lim, Yufeng Wang, Michelle Williams, Peter J. Mohler, Paul M.L. Janssen, Raul Weiss, John D. Hummel, and Vadim V. Fedorov Circ Arrhythm Electrophysiol. 2015 Dec;8(6):1514-7. doi: 10.1161/CIRCEP.115.003064.	Isolated atria (human)	VSD (Di-4-ANBDQBS)	MiCAM ULTIMA
Repolarization Alternans and Ventricular Arrhythmia in a Repaired Tetralogy of Fallot Animal Model. Shuenn]Nan Chiu, Chia]Ti Tsai, Lian]Yu Lin, Shu]Chien Huang, Yih]Sharng Chen, Jou]Kou Wang, Mei]Hwan Wu, Ling]Ping Lai and Jiunn]Lee Lin J Am Heart Assoc. 2015 Dec 11;4(12). pii: e002173. doi: 10.1161/JAHA.115.002173.	Ventricle wedge preparation (dog)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA
Aberrant sodium influx causes cardiomyopathy and atrial fibrillation in mice. Elaine Wan, Jeffrey Abrams, Richard L. Weinberg, Alexander N. Katchman, Joseph Bayne, Sergey I. Zakharov, Lin Yang, John P. Morrow, Hasan Garan and Steven O. Marx J Clin Invest. 2016 Jan 4;126(1):112-22. doi: 10.1172/JCI84669. Epub 2015 Nov 23.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Arrhythmogenic remodeling of β2 versus β1 adrenergic signaling in the human failing heart. Di Lang, Katherine Holzem, Chaoyi Kang, Mengqian Xiao, Hye Jin Hwang, Gregory A. Ewald, Kathryn A. Yamada, and Igor R. Efimov Circ Arrhythm Electrophysiol. 2015 Apr;8(2):409-19. doi: 10.1161/CIRCEP.114.002065. Epub 2015 Feb 11.	Ventricle wedge preparation (human)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
STIM1-Ca2+ signaling modulates automaticity of the mouse sinoatrial node. Hengtao Zhang, Albert Y. Sun, Jong J. Kim, Victoria Graham, Elizabeth A. Finch, Igor Nepliouev, Guiling Zhao, Tianyu Li, W. J. Lederer, Jonathan A. Stiber, Geoffrey S. Pitt, Nenad Bursac and Paul B. Rosenberg Proc Natl Acad Sci U S A. 2015 Oct 13;112(41):E5618-27. doi: 10.1073/pnas.1503847112. Epub 2015 Sep 30.	SAN tissue	CaSD (Rhod-2 AM)	MiCAM ULTIMA
Diet-induced obesity promotes altered remodeling and exacerbated cardiac hypertrophy following pressure overload. Katherine M. Holzem, Joseph T. Marmerstein, Eli J. Madden and Igor R. Efimov Physiol Rep. 2015 Aug;3(8). pii: e12489. doi: 10.14814/phy2.12489.	Isolated heart (mouse)	VSD (RH-237)	MiCAM ULTIMA
Atrial fibrillation driven by micro-anatomic intramural re-entry revealed by simultaneous sub-epicardial and sub-endocardial optical mapping in explanted human hearts. Brian J. Hansen, Jichao Zhao, Thomas A. Csepe, Brandon T. Moore, Ning Li, Laura A. Jayne, Anuradha Kalyanasundaram, Praise Lim, Anna Bratasz, Kimerly A. Powell, Orlando P. Simonetti, Robert S.D. Higgins, Ahmet Kilic, Peter J. Mohler, Paul M.L. Janssen, Raul Weiss, John D. Hummel and Vadim V. Fedorov Eur Heart J. 2015 Jun 8. pii: ehv233. [Epub ahead of print].	Isolated heart (human)	VSD (Di-4-ANBDQBS)	MiCAM ULTIMA (dual camera)
Burst pacemaker activity of the sinoatrial node in sodium-calcium exchanger knockout mice. Angelo G. Torrente, Rui Zhang, Audrey Zaini, Jorge F. Giani, Jeanney Kang, Scott T. Lamp, Kenneth D. Philipson and Joshua I. Goldhaber Proc Natl Acad Sci U S A. 2015 Aug 4;112(31):9769-74. doi: 10.1073/pnas.1505670112. Epub 2015 Jul 20.	SAN/atria tissue	VSD (RH-237)	MiCAM ULTIMA
Engineered heart slices for electrophysiological and contractile studies. Adriana Blazeski, Geran M. Kostecki and Leslie Tung Biomaterials. 2015 Jul;55:119-28. doi: 10.1016/j.biomaterials.2015.03.026. Epub 2015 Apr 17.	Heart slice	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Transplantation of adipose tissue-derived stem cells improves cardiac contractile function and electrical stability in a rat myocardial infarction model. Milan Gautam, Daiki Fujita, Kazuhiro Kimura, Hinako Ichikawa, Atsushi Izawa, Masamichi Hirose, Toshihide Kashihara, Mitsuhiko Yamada, Masafumi Takahashi, Uichi Ikeda and Yuji Shiba J Mol Cell Cardiol. 2015 Apr;81:139-49. doi: 10.1016/j.yjmcc.2015.02.012. Epub 2015 Feb 24.	Isolated heart (rat)	VSD (Di-4-ANEPPS)	MiCAM02
Quantification of the Transmural Dynamics of Atrial Fibrillation by Simultaneous Endocardial and Epicardial Optical Mapping in an Acute Sheep Model. Sarah R. Gutbrod, Richard Walton, Stephen Gilbert, Valentin Meillet, Pierre Jaïs, Mélèze Hocini, Michel Haïssaguerre, Rémi Dubois, Olivier Bernus and Igor Efimov Circ Arrhythm Electrophysiol. 2015 Feb 24. pii: CIRCEP.114.002545. [Epub ahead of print]	Isolated atria (sheep)	VSD (Di-4-ANBDQBS)	MiCAM ULTIMA (dual camera)
Targeting protein tyrosine phosphatase σ after myocardial infarction restores cardiac sympathetic innervation and prevents arrhythmias. R. T. Gardner, L. Wang, B. T. Lang, J. M. Cregg, C. L. Dunbar, W. R. Woodward, J. Silver, C. M. Ripplinger and B. A. Habecker Nat Commun. 2015 Feb 2;6:6235. doi: 10.1038/ncomms7235.	Isolated heart (mouse)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Sodium channels in the Cx43 gap junction perinexus may constitute a cardiac ephapse: an experimental and modeling study. Rengasayee Veeraraghavan, Joyce Lin, Gregory S. Hoeker, James P. Keener, Robert G. Gourdie and Steven Poelzing Pflugers Arch. 2015 Jan 13. [Epub ahead of print]	Isolated heart (guinea pig)	VSD (Di-4-ANEPPS)	MiCAM02
Atherosclerosis exacerbates arrhythmia following myocardial infarction: Role of myocardial inflammation. Nicole M. De Jesus, Lianguo Wang, Anthony W. Herren, Jingjing Wang, Fatemah Shenasa, Donald M. Bers, Merry L. Lindsey and Crystal M. Ripplinger Heart Rhythm. 2015 Jan;12(1):169-78. doi: 10.1016/j.hrthm.2014.10.007. Epub 2014 Oct 7.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart. Lianguo Wang, Nicole M. De Jesus, and Crystal M. Ripplinger J Vis Exp. 2015 Sep 10;(103)	Isolated heart (rabbit)	VSD (RH-237) CaSD (Fluo-5N AM)	MiCAM ULTIMA (dual camera)
Alternating membrane potential/calcium interplay underlies repetitive focal activity in a genetic model of calcium-dependent atrial arrhythmias. Qing Lou, Andriy E. Belevych, Przemysław B. Radwański, Bin Liu, Anuradha Kalyanasundaram, Bjorn C. Knollmann, Vadim V. Fedorov and Sándor Györke J Physiol. 2014 Nov 10. [Epub ahead of print]	Isolated heart (mouse)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Pacing-Induced Non-Uniform Ca2+ Dynamics in Rat Atria Revealed by Rapid-Scanning Confocal Microscopy. Yan Jiang, Hideo Tanaka, Taka-aki Matsuyama, Yoshihisa Yamaoka and Tetsuro Takamatsu Acta Histochem Cytochem. May 1, 2014; 47(2): 59-65. Published online Apr 25, 2014. doi: 10.1267/ahc.14014	Isolated heart (rat)	VSD (Di-4-ANEPPS)	MiCAM02
Ca2+/calmodulin-dependent protein kinase II increases the susceptibility to the arrhythmogenic action potential alternans in spontaneously hypertensive rats. Hirofumi Mitsuyama, Hisashi Yokoshiki, Masaya Watanabe, Kazuya Mizukami, Junichi Shimokawa and Hiroyuki Tsutsui Am J Physiol Heart Circ Physiol. 2014 Jul 15;307(2):H199-206. doi: 10.1152/ajpheart.00387.2012. Epub 2014 May 23.	Isolated heart (rat)	VSD (Di-4-ANEPPS)	MiCAM02
Optical mapping of sarcoplasmic reticulum Ca2+ in the intact heart: ryanodine receptor refractoriness during alternans and fibrillation. Lianguo Wang, Rachel C. Myles, Nicole M. De Jesus, Alex K.P. Ohlendorf, Donald M. Bers and Crystal M. Ripplinger Circ Res. 2014 Apr 25;114(9):1410-21. doi: 10.1161/CIRCRESAHA.114.302505. Epub 2014 Feb 25.	Isolated heart (rabbit)	VSD (RH-237) CaSD (Fluo-5N AM)	MiCAM ULTIMA (dual camera)
Decreased IK1 and increased ryanodine receptor sensitivity synergistically contribute to sustained focal arrhythmia in the intact rabbit heart. Rachel C. Myles, Lianguo Wang, Donald M. Bers and Crystal M. Ripplinger J Physiol. 2014 Sep 5. [Epub ahead of print]	Isolated heart (rabbit)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Mitochondrial depolarization and electrophysiological changes during ischemia in the rabbit and human heart. Matthew S Sulkin, Bastiaan J Boukens, Megan Tetlow, Sarah R Gutbrod, Fu Siong Ng and Igor R. Efimov Am J Physiol Heart Circ Physiol. 2014 Aug 15. pii: ajpheart.00437.2014. [Epub ahead of print]	Isolated heart (mouse)	VSD (RH-237 and TMRM)	MiCAM ULTIMA (dual camera)
Upregulation of adenosine A1 receptors facilitates sinoatrial node dysfunction in chronic canine heart failure by exacerbating nodal conduction abnormalities revealed by novel dual-sided intramural optical mapping. Qing Lou, Brian J. Hansen, Olga Fedorenko, Thomas A. Csepe, Anuradha Kalyanasundaram, Ning Li, Lori T. Hage, Alexey V. Glukhov, George E. Billman, Raul Weiss, Peter J. Mohler, Sándor Györke, Brandon J. Biesiadecki, Cynthia A. Carnes, PharmD and Vadim V. Fedorov Circulation. 2014 Jul 22;130(4):315-24. doi: 10.1161/CIRCULATIONAHA.113.007086. Epub 2014 May 16.	Isolated heart (dog)	VSD (Di-4-ANBDQBS)	MiCAM ULTIMA (dual camera)
3D multifunctional integumentary membranes for spatiotemporal cardiac measurements and stimulation across the entire epicardium. Lizhi Xu, Sarah R. Gutbrod, Andrew P. Bonifas, Yewang Su, Matthew S. Sulkin, Nanshu Lu, Hyun-Joong Chung, Kyung-In Jang, Zhuangjian Liu, Ming Ying, Chi Lu, R. Chad Webb, Jong-Seon Kim, Jacob I. Laughner, Huanyu Cheng, Yuhao Liu, Abid Ameen, Jae-Woong Jeong, Gwang-Tae Kim, Yonggang Huang, Igor R. Efimov and John A. Rogers Nat Commun. 2014 Feb 25;5:3329. doi: 10.1038/ncomms4329.	Isolated heart (rabbit)	VSD (Di-4-ANEPPS or RH-237)	MiCAM ULTIMA
c-Src Kinase Inhibition Reduces Arrhythmia Inducibility and Connexin43 Dysregulation After Myocardial Infarction. Cody A. Rutledge, Fu Siong Ng, Matthew S. Sulkin, Ian D. Greener, Artem M. Sergeyenko, Hong Liu, Joanna Gemel, Eric C. Beyer, Ali A. Sovari, Igor R. Efimov and Samuel C. Dudley J Am Coll Cardiol. 2014 Mar 11;63(9):928-34. doi: 10.1016/j.jacc.2013.10.081. Epub 2014 Jan 8.	Isolated heart (mouse)	VSD (RH-237)	MiCAM ULTIMA
Selective inhibition of late sodium current suppresses ventricular tachycardia and fibrillation in intact rat hearts. Arash Pezhouman, Sepideh Madahian, Hayk Stepanyan, Hayk Ghukasyan, Zhilin Qu, Luiz Belardinelli and Hrayr S. Karagueuzian Heart Rhythm. 2014 Mar;11(3):492-501. doi: 10.1016/j.hrthm.2013.11.026. Epub 2013 Nov 28.	Isolated heart (rat)	VSD (RH-237)	MiCAM ULTIMA
Transmural ultrasound imaging of thermal lesion and action potential changes in perfused canine cardiac wedge preparations by high intensity focused ultrasound ablation. Ziqi Wu, Madhu S. R. Gudur and Cheri X. Deng PLoS One. 2013 Dec 12;8(12):e82689. doi: 10.1371/journal.pone.0082689. eCollection 2013.	Isolated heart (dog)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Functional suppression of Kcnq1 leads to early sodium channel remodelling and cardiac conduction system dysmorphogenesis. Angel J. de la Rosa, Jorge N. Domínguez, David Sedmera, Bara Sankova, Leif Hove-Madsen, Diego Franco and Amelia E. Aránega Cardiovasc Res. 2013 Jun 1;98(3):504-14. doi: 10.1093/cvr/cvt076. Epub 2013 Mar 29.	Isolated heart (mouse embryo)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Diabetic hyperglycaemia activates CaMKII and arrhythmias by O-linked glycosylation. Jeffrey R. Erickson, Laetitia Pereira, Lianguo Wang, Guanghui Han, Amanda Ferguson, Khanha Dao, Ronald J. Copeland, Florin Despa, Gerald W. Hart, Crystal M. Ripplinger and Donald M. Bers Nature. 2013 Oct 17;502(7471):372-6. doi: 10.1038/nature12537. Epub 2013 Sep 29.	Isolated heart (rat)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Multi-Stage Electrotherapy Delivered Through Chronically Implanted Leads Terminates Atrial Fibrillation with Lower Energy than a Single Biphasic Shock. Ajit H. Janardhan, Sarah R. Gutbrod, Wenwen Li, Di Lang, Richard B. Schuessler and Igor R. Efimov J Am Coll Cardiol. 2013 Sep 26. pii: S0735-1097(13)05356-4. doi: 10.1016/j.jacc.2013.07.098. [Epub ahead of print]	Isolated heart (dog)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Sinoatrial node reentry in a canine chronic left ventricular infarct model: role of intranodal fibrosis and heterogeneity of refractoriness. Alexey V. Glukhov, Lori T. Hage, Brian J. Hansen, Adriana Pedraza-Toscano, Pedro Vargas-Pinto, Robert L. Hamlin, Raul Weiss, Cynthia A. Carnes, George E. Billman and Vadim V. Fedorov Circ Arrhythm Electrophysiol. 2013 Oct 1;6(5):984-94. doi: 10.1161/CIRCEP.113.000404. Epub 2013 Aug 19.	Isolated heart (dog)	VSD (Di-4-ANBDQBS)	MiCAM ULTIMA
Molecular basis of selective atrial fibrosis due to overexpression of transforming growth factor-β1. Dolkun Rahmutula, Gregory M. Marcus, Emily E. Wilson, Chun-Hua Ding, Yuanyuan Xiao, Agnes C. Paquet, Rebecca Barbeau, Andrea J. Barczak, David J. Erle and Jeffrey E. Olgin Cardiovasc Res. 2013 Sep 1;99(4):769-79. doi: 10.1093/cvr/cvt074. Epub 2013 Apr 23.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Functional roles of K_ATP channel subunits in metabolic inhibition. Alexey V. Glukhova, Keita Uchida, Igor R. Efimov, Colin G. Nichols J Mol Cell Cardiol. 2013 Apr 23;62C:90-98. doi: 10.1016/j.yjmcc.2013.04.016. [Epub ahead of print]	Isolated heart (mouse)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Mesenchymal Stem Cell Transplantation Mitigates Electrophysiological Remodeling in a Rat Model of Myocardial Infarction. Patrick F.H. Lai, Brian K. Panama, Stéphane Massé, Guangming Li, Yaoguang Zhang, Marjan Kusha, Talha A. Farid, John Asta, Peter H. Backx, Terrence M. Yau and Kumaraswamy Nanthakumar J Cardiovasc Electrophysiol. 2013 Apr 8. doi: 10.1111/jce.12162. [Epub ahead of print]	Isolated heart (rat)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA (dual camera)
Calcium measurements from whole heart using Rhod-2. Bum-Rak Choi Methods Mol Biol. 2013;937:217-28.	Isolated heart (rabbit, guinea pig, mouse)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Pitx2 confers left morphological, molecular, and functional identity to the sinus venosus myocardium. Grazia Ammirabile, Alessandra Tessari, Viviana Pignataro, Dorota Szumska, Fabio Sutera Sardo, Jiri Benes, Jr, Mariangela Balistreri, Shoumo Bhattacharya, David Sedmera and Marina Campione Cardiovasc Res. 2012 Feb 1;93(2):291-301. doi: 10.1093/cvr/cvr314. Epub 2011 Nov 23.	Isolated heart (mouse embryo)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
The effect of connexin40 deficiency on ventricular conduction system function during development. Barbora Sankova, Jiri Benes Jr, Eliska Krejci, Laurent Dupays, Magali Theveniau-Ruissy, Lucile Miquerol and David Sedmera Cardiovasc Res. 2012 Sep 1;95(4):469-79. doi: 10.1093/cvr/cvs210. Epub 2012 Jun 27.	Isolated heart (embryo)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Identification and Functional Characterization of Cardiac Pacemaker Cells in Zebrafish. Federico Tessadori, Jan Hendrik van Weerd, Silja B. Burkhard, Arie O. Verkerk, Emma de Pater, Bastiaan J. Boukens, Aryan Vink, Vincent M. Christoffels and Jeroen Bakkers PLoS One. 2012;7(10):e47644. doi: 10.1371/journal.pone.0047644. Epub 2012 Oct 16.	Isolated heart (zebrafish)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Processing and analysis of cardiac optical mapping data obtained with potentiometric dyes. Jacob I Laughner, Fu Siong Ng, Matthew S Sulkin, R Martin Arthur and Igor R Efimov Am J Physiol Heart Circ Physiol. 2012 Oct;303(7):H753-65. doi: 10.1152/ajpheart.00404.2012. Epub 2012 Jul 20.	Isolated heart (rabbit)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Focal but reversible diastolic sheet dysfunction reflects regional calcium mishandling in dystrophic mdx mouse hearts. Ya-Jian Cheng, Di Lang, Shelton D Caruthers, Igor R Efimov, Junjie Chen and Samuel A Wickline Am J Physiol Heart Circ Physiol. 2012 Sep 1;303(5):H559-68. doi: 10.1152/ajpheart.00321.2012. Epub 2012 Jul 9.	Isolated heart (mouse)	CaSD (Rhod-2 AM)	MiCAM ULTIMA
Oscillation in Cycle Length Induces Transient Discordant and Steady-State Concordant Alternans in the Heart. Seth H. Weinberg and Leslie Tung PLoS One. 2012;7(7):e40477. Epub 2012 Jul 5.	Isolated heart (guinea pig)	VSD (Di-4-ANEPPS) CaSD (Rhod-2 AM)	MiCAM ULTIMA
Construction of 3D MR image-based computer models of pathologic hearts, augmented with histology and optical fluorescence imaging to characterize action potential propagation. Mihaela Pop, Maxime Sermesant, Garry Liu, Jatin Relan, Tommaso Mansi, Alan Soong, Jean-Marc Peyrat, Michael V. Truong, Paul Fefer, Elliot R. McVeigh, Herve Delingette, Alexander J. Dick, Nicholas Ayache, Graham A. Wright Med Image Anal. 2012 Feb;16(2):505-23. Epub 2011 Dec 6.	Isolated heart (pig)	VSD (Di-4-ANEPPS)	MiCAM02 (dual camera)
Conduction and refractory disorders in the diabetic atrium. Masaya Watanabe, Hisashi Yokoshiki, Hirofumi Mitsuyama, Kazuya Mizukami, Taisuke Ono, and Hiroyuki Tsutsui Am J Physiol Heart Circ Physiol. 2012 Jul;303(1):H86-95. Epub 2012 May 4.	Isolated heart (rat)	VSD (Di-4-ANEPPS)	MiCAM02
Local β-Adrenergic Stimulation Overcomes Source-Sink Mismatch to Generate Focal Arrhythmia. Rachel C. Myles, Lianguo Wang, Chaoyi Kang, Donald M. Bers and Crystal M. Ripplinger Circ Res. 2012 May 25;110(11):1454-64. Epub 2012 Apr 26.	Isolated heart (rabbit)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Enhanced sensitivity of aged fibrotic hearts to angiotensin II- and hypokalemia-induced early afterdepolarization-mediated ventricular arrhythmias. Aneesh Bapat, Thao P. Nguyen, Jong-Hwan Lee Ali A. Sovari, Michael C. Fishbein, James N. Weiss and Hrayr S. Karagueuzian Am J Physiol Heart Circ Physiol. 2012 Jun 1;302(11):H2331-40. Epub 2012 Mar 30.	Isolated heart (rat)	VSD (RH-237)	MiCAM ULTIMA
A Novel, Minimally Invasive, Segmental Myocardial Infarction with a Clear Healed Infarct Borderzone in Rabbits. Ohad Ziv, Lorraine Schofield, Emily Lau, Leonard Chaves, Dyviang Patel, Paul Jeng, Xuwen Peng, Bum-Rak Choi and Gideon Koren Am J Physiol Heart Circ Physiol. 2012 Mar 23. [Epub ahead of print]	Isolated heart (rabbit)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Conduction remodeling in human end-stage nonischemic left ventricular cardiomyopathy. Alexey V. Glukhov, Vadim V. Fedorov, Paul W. Kalish, Vinod K. Ravikumar, Qing Lou, Deborah Janks, Richard B. Schuessler, Nader Moazami and Igor R. Efimov Circulation. 2012 Apr 17;125(15):1835-47. Epub 2012 Mar 12.	Isolated heart (dog and human)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Three Potential Mechanisms for Failure of HIFU Ablation in Cardiac Tissue. Jacob I. Laughner, Matthew S. Sulkin, Ziqi Wu, Cheri X. Deng and Igor R. Efimov Circ Arrhythm Electrophysiol. 2012 Feb 9. [Epub ahead of print]	Isolated heart (rabbit)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Inter-model consistency and complementarity: Learning from ex-vivo imaging and electrophysiological data towards an integrated understanding of cardiac physiology. O. Camara, M. Sermesant, P. Lamata, L. Wang,M. Pop, J. Relan, M. De Craene, H. Delingette, H. Liu, S. Niederer, A. Pashaei, G. Plank, D. Romero R. Sebastian, K.C.L. Wong, H. Zhang, N. Ayache, A.F. Frangi, P. Shi, N.P. Smith G.A. Wright Prog Biophys Mol Biol. 2011 Oct;107(1):122-33. Epub 2011 Jul 20.	Isolated heart (pig)	VSD (Di-4-ANEPPS)	MiCAM02 (dual camera)
Overexpression of cAMP-response element modulator causes abnormal growth and development of the atrial myocardium resulting in a substrate for sustained atrial fibrillation in mice. Paulus Kirchhof, Eloi Marijond, Larissa Fabritza, Na Li, Wei Wang, Tiannan Wang, Kirsten Schulte, Juliane Hanstein, Jan S. Schulte, Mathis Vogel, Nathalie Mougenot, Sandra Laakmann, Lisa Fortmueller, Jens Eckstein, Sander Verheule, Sven Kaese, Ariane Staab, Stephanie Grote-Wessels, Ulrich Schotten, Ghassan Moubarak, Xander H.T. Wehrens, Wilhelm Schmitz, Stéphane Hatem, Frank Ulrich Müller. Int J Cardiol. 2011 Nov 15. [Epub ahead of print]	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Notch signaling regulates murine atrioventricular conduction and the formation of accessory pathways. Stacey Rentschler, Brett S. Harris, Laura Kuznekoff, Rajan Jain, Lauren Manderfield, Min Min Lu, Gregory E. Morley, Vickas V. Patel and Jonathan A. Epstein J Clin Invest. 2011 Feb;121(2):525-33. doi: 10.1172/JCI44470. Epub 2011 Jan 25.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Transmural Heterogeneity and Remodeling of Ventricular Excitation-Contraction Coupling in Human Heart Failure. Qing Lou, Vadim V. Fedorov, Alexey V. Glukhov, Nader Moazami, Vladimir G. Fast and Igor R. Efimov Circulation. 2011 May 3;123(17):1881-90. Epub 2011 Apr 18.	Isolated heart (human)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Spatiotemporal control of heart rate in a rabbit heart. Di Lang, Valentin Petrov, Qing Lou, Grigory Osipov and Igor R. Efimov J Electrocardiol. 2011 Nov-Dec;44(6):626-34. Epub 2011 Sep 19.	Isolated heart (rabbit)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Phosphatase-resistant gap junctions inhibit pathological remodeling and prevent arrhythmias. Benjamin F. Remo, Jiaxiang Qu, Frank M. Volpicelli, Steven Giovannone, Daniel Shin, Joshua Lader, Fang-yu Liu, Jie Zhang, Danielle S. Lent, Gregory E. Morley and Glenn I. Fishman Circ Res. 2011 Jun 10;108(12):1459-66. Epub 2011 Apr 28.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Anatomic localization and autonomic modulation of atrioventricular junctional rhythm in failing human hearts. Vadim V. Fedorov, Christina M. Ambrosi, Geran Kostecki, William J. Hucker, Alexey V. Glukhov, Joseph P. Wuskell, Leslie M. Loew, Nader Moazami and Igor R. Efimov Circ Arrhythm Electrophysiol. 2011 Aug 1;4(4):515-25. Epub 2011 Jun 6.	Isolated heart (human)	VSD (Di-4-ANBDQBS)	MiCAM ULTIMA (dual camera)
Role of Pyk2 in cardiac arrhythmogenesis. Di Lang, Alexey V. Glukhov, Tatiana Efimova and Igor R. Efimov Am J Physiol Heart Circ Physiol. 2011 Sep;301(3):H975-83. Epub 2011 Jun 10.	Isolated heart (mouse)	VSD (RH-237)	MiCAM ULTIMA
Spatiotemporal electrophysiological changes in a murine ablation model. Scott A. Bernstein, Srikant Duggirala, Michael Floberg, Pehr Elfvendal, Laura M. Kuznekoff, Joshua M. Lader, Carolina Vasquez and Gregory E. Morley Europace. 2011 Oct;13(10):1494-500. Epub 2011 Jun 28.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
In-vitro recording of adult zebrafish heart electrocardiogram - a platform for pharmacological testing. Chia-Ti Tsai, Cho-Kai Wu, Fu-Tien Chiang, Chuen-Den Tseng, Jen-Kuang Lee, Chih-Chieh Yu, Yi-Chih Wang, Ling-Ping Lai, Jiunn-Lee Lin and Juey-Jen Hwang Clin Chim Acta. 2011 Oct 9;412(21-22):1963-7. Epub 2011 Jul 8.	Isolated heart (zebrafish)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Remodeling of atrial ATP-sensitive K+ channels in a model of salt-induced elevated blood pressure. Joshua M. Lader, Carolina Vasquez, Li Bao, Karen Maass, Jiaxiang Qu, Eirini Kefalogianni, Glenn I. Fishman, William A. Coetzee and Gregory E. Morley Am J Physiol Heart Circ Physiol. 2011 Sep;301(3):H964-74. Epub 2011 Jul 1.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Effects of KATP channel openers diazoxide and pinacidil in coronary-perfused atria and ventricles from failing and non-failing human hearts. Vadim V. Fedorov, Alexey V. Glukhov, Christina M. Ambrosi, Geran Kostecki, Roger Chang, Deborah Janks, Richard B. Schuessler, Nader Moazami, Colin G. Nichols and Igor R. Efimov J Mol Cell Cardiol. 2011 Aug;51(2):215-25. Epub 2011 May 7.	Isolated heart (human)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA (dual camera)
Multiparametric Optical Mapping of the Langendorff-perfused Rabbit Heart (Video Article). Qing Lou, Wenwen Li and Igor R. Efimov J. Vis. Exp. (55), e3160, DOI: 10.3791/3160 (2011)	Isolated heart (rabbit)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart (Video Article). Di Lang, Matthew Sulkin, Qing Lou and Igor R. Efimov J. Vis. Exp. (55), e3275, DOI: 10.3791/3275 (2011)	Isolated heart (mouse)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Small-conductance calcium-activated potassium channel and recurrent ventricular fibrillation in failing rabbit ventricles. Su-Kiat Chua, Po-Cheng Chang, Mitsunori Maruyama, Isik Turker, Tetsuji Shinohara, Mark J. Shen, Zhenhui Chen, Changyu Shen, Michael Rubart-von der Lohe, John C. Lopshire, Masahiro Ogawa, James N. Weiss, Shien-Fong Lin, Tomohiko Ai and Peng-Sheng Chen Circ Res. 2011 Apr 15;108(8):971-9. Epub 2011 Feb 24.	Isolated heart (rabbit)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
FKBP12 is a critical regulator of the heart rhythm and the cardiac voltage-gated sodium current in mice. Mitsunori Maruyama, Bai-Yan Li, Hanying Chen, Xuehong Xu, Long-Sheng Song, Silvia Guatimosim, Wuqiang Zhu, Weidong Yong, Wenjun Zhang, Guixue Bu, Shien-Fong Lin, Michael C. Fishbein, W. Jonathan Lederer, John H. Schild, Loren J. Field, Michael Rubart, Peng-Sheng Chen and Weinian Shou Circ Res. 2011 Apr 29;108(9):1042-52. Epub 2011 Mar 3.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA (dual camera)
Intracellular calcium and the mechanism of anodal supernormal excitability in langendorff perfused rabbit ventricles. Boyoung Joung, Hyung-Wook Park, Mitsunori Maruyama, Liang Tang, Juan Song, Seongwook Han, Gianfranco Piccirillo, James N. Weiss, Shien-Fong Lin and Peng-Sheng Chen Circ J. 2011 Apr;75(4):834-43. Epub 2011 Feb 2.	Isolated heart (rabbit)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Delayed afterdepolarization in intact canine sinoatrial node as a novel mechanism for atrial arrhythmia. Boyoung Joung, Hong Zhang, Tetsuji Shinohara, Mitsunori Maruyama, Seongwook Han, Daehyeok Kim, Eue-Keun Choi, Young-Keun On, Shien-Fong Lin and Peng-Sheng Chen J Cardiovasc Electrophysiol. 2011 Apr;22(4):448-54. doi: 10.1111/j.1540-8167.2010.01905.x. Epub 2010 Oct 6.	Isolated heart (dog)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Genesis of phase 3 early afterdepolarizations and triggered activity in acquired long-QT syndrome. Mitsunori Maruyama, Shien-Fong Lin, Yuanfang Xie, Su-Kiat Chua, Boyoung Joung, Seongwook Han, Tetsuji Shinohara, Mark J. Shen, Zhilin Qu, James N. Weiss and Peng-Sheng Chen Circ Arrhythm Electrophysiol. 2011 Feb;4(1):103-11. Epub 2010 Nov 15.	Isolated heart (rabbit)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Personalisation of a Cardiac Electrophysiology Model using Optical Mapping and MRI for Prediction of Changes with Pacing. Jatin Relan, Mihaela Pop, H Delingette, G Wright, N Ayache and M Sermesant IEEE Trans Biomed Eng. 2011 Jan 20. [Epub ahead of print]	Isolated heart (pig)	VSD (Di-4-ANEPPS)	MiCAM02 (dual camera)
The role of the calcium and the voltage clocks in sinoatrial node dysfunction. Boyoung Joung, Peng-Sheng Chen and Shien-Fong Lin Yonsei Med J. 2011 Mar 1;52(2):211-9.	Isolated heart (dog)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Assessment of cardiac conduction: basic principles of optical mapping. Chunhua Ding and Thomas H. Everett Methods Mol Biol. 2010;660:239-52.	Isolated heart (mouse)	VSD (Di-4-ANEPPS or PGH-I)	MiCAM02
PITX2c is Expressed in the Adult Left Atrium, and Reducing Pitx2c Expression Promotes Atrial Fibrillation Inducibility and Complex Changes in Gene Expression. Paulus Kirchhof, Peter C. Kahr, Sven Kaese, Ilaria Piccini, Ismail Vokshi, Hans-Heinrich Scheld, Heinrich Rotering, Lisa Fortmueller, Sandra Laakmann, Sander Verheule, Ulrich Schotten, Larissa Fabritz and Nigel A. Brown Circ Cardiovasc Genet. 2011 Apr;4(2):123-33. Epub 2011 Jan 31.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Alternans of diastolic intracellular calcium elevation as the mechanism of bidirectional ventricular tachycardia in a rabbit model of Andersen-Tawil syndrome. Mitsunori Maruyama, Shien-Fong Lin and Peng-Sheng Chen Heart Rhythm. 2010 Dec 15. [Epub ahead of print]	Isolated heart (rabbit)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Ca2+ clock malfunction in a canine model of pacing-induced heart failure. Tetsuji Shinohara, Hyung-Wook Park, Seongwook Han, Mark J. Shen, Mitsunori Maruyama, Daehyeok Kim, Peng-Sheng Chen and Shien-Fong Lin Am J Physiol Heart Circ Physiol. 2010 Dec;299(6):H1805-11. Epub 2010 Oct 1.	Isolated heart (dog)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Calcium dynamics and the mechanisms of atrioventricular junctional rhythm. Daehyeok Kim, Tetsuji Shinohara, Boyoung Joung, Mitsunori Maruyama, Eue-Keun Choi, Young Keun On, Seongwook Han, Michael C. Fishbein, Shien-Fong Lin and Peng-Sheng Chen J Am Coll Cardiol. 2010 Aug 31;56(10):805-12.	Isolated heart (mongrel dog)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Tachybradycardia in the isolated canine right atrium induced by chronic sympathetic stimulation and pacemaker current inhibition. Boyoung Joung, Tetsuji Shinohara, Hong Zhang, Daehyeok Kim, Eue-Keun Choi, Yong-Keun On, Gianfranco Piccirillo, Peng-Sheng Chen and Shien-Fong Lin Am J Physiol Heart Circ Physiol. 2010 Sep;299(3):H634-42. Epub 2010 Jul 2.	Isolated heart (dog)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Cytosolic calcium accumulation and delayed repolarization associated with ventricular arrhythmias in a guinea pig model of Andersen-Tawil syndrome. Przemysław B. Radwański, Rengasayee Veeraraghavan and Steven Poelzing Heart Rhythm. 2010 Oct;7(10):1428-1435.e1. Epub 2010 Apr 7.	Isolated heart (guinea pig)	VSD (Di-4-ANEPPS) CaSD (Indo-1)	MiCAM02 (dual camera)
Anatomy and electrophysiology of the human AV node. Thomas Kurian, Christina Ambrosi, William Hucker, Vadim V. Fedorov and Igor R. Efimov Pacing Clin Electrophysiol. 2010 Jun 1;33(6):754-62. Epub 2010 Feb 18.	Isolated heart (human)	VSD	MiCAM ULTIMA
In vivo assessment of the electrophysiological integration and arrhythmogenic risk of myocardial cell transplantation strategies. Lior Gepstein, Chunhua Ding, Dolkun Rehemedula, Emily E. Wilson, Lior Yankelson, Oren Caspi, Amira Gepstein, Irit Huber and Jeffery E. Olgin Stem Cells. 2010 Dec;28(12):2151-61.	Isolated heart (rat)	VSD (PGH I)	MiCAM02 (dual camera)
Termination of sustained atrial flutter and fibrillation using low-voltage multiple-shock therapy. Christina M. Ambrosi, Crystal M. Ripplinger, Igor R. Efimov and Vadim V. Fedorov Heart Rhythm. 2011 Jan;8(1):109-10.	Isolated heart (rabbit)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Optical mapping of the isolated coronary-perfused human sinus node. Vadim V. Fedorov, Alexey V. Glukhov, Roger Chang, Geran Kostecki, Hyuliya Aferol, William J. Hucker, Joe Wuskell, Leslie M. Loew, Richard B. Schuessler, Nader Moazami and Igor R. Efimov J Am Coll Cardiol. 2010 Oct 19;56(17):1386-94.	Isolated heart (human)	VSD (Di-4-ANBDQBS)	MiCAM ULTIMA
ST segment elevation by current-to-load mismatch: an experimental and computational study. Mark G. Hoogendijk, Mark Potse, Alain Vinet, Jacques M.T. de Bakker and Ruben Coronel Heart Rhythm. 2011 Jan;8(1):111-8. Epub 2010 Oct 30.	Isolated heart (pig)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Knock-in gain-of-function sodium channel mutation prolongs atrial action potentials and alters atrial vulnerability. Andreas Blana, Sven Kaese, Lisa Fortmüller, Sandra Laakmann, Dierk Damke, Kelly van Bragt, Jens Eckstein, Ilaria Piccini, Uwe Kirchhefer, Stanley Nattel, Günter Breithardt, Peter Carmeliet, Edward Carmeliet, Ulrich Schotten, Sander Verheule, Paulus Kirchhof and Larissa Fabritz Heart Rhythm. 2010 Dec;7(12):1862-9. Epub 2010 Aug 19.	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Complex interactions between the sinoatrial node and atrium during reentrant arrhythmias in the canine heart. Vadim V. Fedorov, Roger Chang, Alexey V. Glukhov, Geran Kostecki, Deborah Janks, Richard B. Schuessler and Igor R. Efimov Circulation. 2010 Aug 24;122(8):782-9. Epub 2010 Aug 9.	Isolated heart (mongrel dog)	VSD (Di-4-ANEPPS or RH-237)	MiCAM ULTIMA
Functional anatomy of the murine sinus node: high-resolution optical mapping of ankyrin-B heterozygous mice. Alexey V. Glukhov, Vadim V. Fedorov, Mark E. Anderson, Peter J. Mohler and Igor R. Efimov Am J Physiol Heart Circ Physiol. 2010 Aug;299(2):H482-91. Epub 2010 Jun 4.	Isolated heart (mouse)	VSD (RH-237)	MiCAM ULTIMA
Differential K(ATP) channel pharmacology in intact mouse heart. Alexey V. Glukhov, Thomas P. Flagg, Vadim V. Fedorov, Igor R. Efimov and Colin G. Nichols J Mol Cell Cardiol. 2010 Jan;48(1):152-60. Epub 2009 Sep 8.	Isolated heart (mouse)	VSD (RH-237)	MiCAM ULTIMA
Electrophysiological Studies of Transgenic Long QT Type 1 and 2 Rabbits Reveal Genotype-Specific Differences in Ventricular Refractoriness and His Conduction. Katja E. Odening, Malcolm Kirk, Michael Brunner, Ohad Ziv, Peem Lorvidhaya, Gong Xin Liu, Lorraine Schofield, Leonard Chaves, Xuwen Peng, Manfred Zehender, Bum-Rak Choi and Gideon Koren Am J Physiol Heart Circ Physiol. 2010 Jun 25. [Epub ahead of print]	Isolated heart (rabbit)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Transmural dispersion of repolarization in failing and nonfailing human ventricle. Alexey V. Glukhov, Vadim V. Fedorov, Qing Lou, Vinod K. Ravikumar, Paul W. Kalish, Richard B. Schuessler, Nader Moazami and Igor R. Efimov Circ Res. 2010 Mar 19;106(5):981-91. Epub 2010 Jan 21.	Isolated heart (human)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Electrical Activation of Sinus Venosus Myocardium and Expression Patterns of RhoA and Isl-1 in the Chick Embryo. Rebecca Vicente-Steijn, Denise P. Kolditz, Edris A.F. Mahtab, Saïd F.A. Askar, Noortje A.M. Bax, Linda M. Van Der Graaf, Lambertus J. Wisse, Robert Passier, Daniël A. Pijnappels, Martin J. Schalij, Robert E. Poelmann, Adriana C. Gittenberger-De Groot and Monique R.M. Jongbloed J Cardiovasc Electrophysiol. 2010 Apr 29. [Epub ahead of print]	Isolated heart (chick embryo)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Pharmacological Activation of I_Kr Impairs Conduction in Guinea Pig Hearts. Anders Peter Larsen, Søren-Peter Olesen, Morten Grunnet and Steven Poelzing J Cardiovasc Electrophysiol. Published Online: Feb 16 2010	Isolated heart (guinea pig)	VSD (Di-4-ANEPPS)	MiCAM02 (dual camera)
High-resolution optical mapping of ventricular tachycardia in rats with chronic myocardial infarction. Chunhua Ding, Lior Gepstein, Duy Thai Nguyen, Emily Wilson, George Hulley, Andrew Beaser, Randall J. Lee and Jeffrey Olgin Pacing Clin Electrophysiol. 2010 Jun 1;33(6):687-95.	Isolated heart (rat)	VSD (PGH I)	MiCAM02
Pirfenidone mitigates left ventricular fibrosis and dysfunction after myocardial infarction and reduces arrhythmias. Duy T. Nguyen, Chunhua Ding, Emily Wilson, Gregory M. Marcus and Jeffrey E. Olgin Heart Rhythm. 2010 Apr 27.	Isolated heart (rat)	VSD	MiCAM ULTIMA
Effects of mechanical loading on early conduction system differentiation in the chick. Barbora Sankova, Jakub Machalek and David Sedmera Am J Physiol Heart Circ Physiol. 2010 May;298(5):H1571-6.	Isolated heart (chick embryo)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Mapping cardiac pacemaker circuits: methodological puzzles of the sinoatrial node optical mapping. Igor R. Efimov, Vadim V. Fedorov, Boyoung Joung and Shien-Fong Lin Circ Res. 2010 Feb 5;106(2):255-71.	Isolated heart (rabbit, dog)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Transmural Dispersion of Repolarization in Failing and Nonfailing Human Ventricle. Alexey V. Glukhov, Vadim V. Fedorov, Qing Lou, Vinod K. Ravikumar, Paul W. Kalish, Richard B. Schuessler, Nader Moazami and Igor R. Efimov Circ Res.2010; 0:	Isolated heart (human)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Induction of atrial ectopic beats with calcium release inhibition: Local hierarchy of automaticity in the right atrium. Tetsuji Shinohara, Boyoung Joung, Daehyeok Kim, Mitsunori Maruyama, Hsiang-Ning Luk, Peng-Sheng Chen and Shien-Fong Lin Heart Rhythm. 2010 Jan;7(1):110-116	Isolated heart (mongrel dog)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Origin of complex behaviour of spatially discordant alternans in a transgenic rabbit model of type 2 long QT syndrome. Ohad Ziv, Eduardo Morales, Yoon-kyu Song, Xuwen Peng, Katja E Odening, Alfred E Buxton, Alain Karma, Gideon Koren and Bum-Rak Choi J Physiol. 2009 Oct 1;587(Pt 19):4661-80.	Isolated heart (rabbit)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Diastolic Intracellular Calcium-Membrane Voltage Coupling Gain and Postshock Arrhythmias. Role of Purkinje Fibers and Triggered Activity. Mitsunori Maruyama, Boyoung Joung, Liang Tang, Tetsuji Shinohara, Young-Keun On, Seongwook Han, Eue-Keun Choi, Dae-Hyeok Kim, Mark J. Shen, James N. Weiss, Shien-Fong Lin and Peng-Sheng Chen Circ Res. 2010 Feb 5;106(2):399-408. Epub 2009 Nov 19.	Isolated heart (rabbit)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Optical mapping of VF in isolated swine hearts with scars. Mihaela Pop, Alan Soong, John M. Hudson, Paul Fefer, Alexander Dick and Graham A. Wright Biophysical Journal, Volume 96, Issue 3, Pages 262a-262a	Isolated heart (pig)	VSD (Di-4-ANEPPS)	MiCAM02
Mechanisms of sinoatrial node dysfunction in a canine model of pacing-induced atrial fibrillation. Boyoung Joung, Shien-Fong Lin, Zhenhui Chen, Patrick S. Antoun, Mitsunori Maruyama, Seongwook Han, Gianfranco Piccirillo, Marcelle Stucky, Douglas P. Zipes, FHRS, Peng-Sheng Chen and Mithilesh Kumar Das> Heart Rhythm. 2010 Jan;7(1):88-95. Epub 2009 Sep 16.	Isolated heart (dog)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Gap junction remodeling and spironolactone-dependent reverse remodeling in the hypertrophied heart. Jiaxiang Qu, Frank M. Volpicelli, Luis I. Garcia, Nefthi Sandeep, Jie Zhang, Lucrecia Márquez-Rosado, Paul D. Lampe and Glenn I. Fishman Circ Res. 2009 Feb 13;104(3):365-71.	Isolated heart (mouse)	VSD	MiCAM ULTIMA
Simultaneous optical mapping of intracellular free calcium and action potentials from Langendorff perfused hearts. Guy Salama and Seong-min Hwang Curr Protoc Cytom. 2009 Jul;Chapter 12:Unit 12.17.	Isolated heart (guinea pig)	VSD (PGH I) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Inhomogeneous distribution of action potential characteristics in the rabbit sino-atrial node revealed by voltage imaging. Haruko Masumiya, Yoshitaka Oku and Yasumasa Okada J Physiol Sci. 2009 May;59(3):227-41.	Isolated heart (rabbit)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Fusion of optical imaging and MRI for the evaluation and adjustment of macroscopic models of cardiac electrophysiology: a feasibility study. Mihaela Pop, Maxime Sermesant, Damien Lepiller, Michael V. Truong, Elliot R. McVeigh, Eugene Crystal, Alexander Dick, Herve Delingette, Nicholas Ayache and Graham A. Wright Med Image Anal. 2009 Apr;13(2):370-80.	Isolated heart (pig)	VSD (Di-4-ANEPPS)	MiCAM02 (dual camera)
Intracellular calcium dynamics and acceleration of sinus rhythm by beta-adrenergic stimulation. Boyoung Joung, Liang Tang, Mitsunori Maruyama, Seongwook Han, Zhenhui Chen, Marcelle Stucky, Larry R. Jones, Michael C. Fishbein, James N. Weiss, Peng-Sheng Chen and Shien-Fong Lin Circulation. 2009 Feb 17;119(6):788-96.	Isolated heart (mongrel dog)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Cardioprotective effects of propofol in isolated ischemia-reperfused guinea pig hearts: role of KATP channels and GSK-3beta. Noriko Kamada, Noriaki Kanaya, Naoyuki Hirata, Saori Kimura and Akiyoshi Namiki Can J Anaesth. 2008 Sep;55(9):595-605.	Isolated heart (guinea pig)	VSD (Di-4-ANEPPS)	MiCAM02
Superiority of biphasic over monophasic defibrillation shocks is attributable to less intracellular calcium transient heterogeneity. Gyo-Seung Hwang, Liang Tang, Boyoung Joung, Norishige Morita, Hideki Hayashi, Hrayr S. Karagueuzian, James N. Weiss, Shien-Fong Lin and Peng-Sheng Chen J Am Coll Cardiol. 2008 Sep 2;52(10):828-35.	Isolated heart (rabbit)	VSD (RH-237) CaSD (Rhod-2 AM)	MiCAM ULTIMA (dual camera)
Abnormal Myocardial and Coronary Vasculature Development in Experimental Pypoxia. Ondrej Nanka, Petra Krizova, Michal Fikrle, Michal Tuma, Milan Blaha, Milos Grim and David Sedmera Anat Rec (Hoboken). 2008 Oct;291(10):spc1.	Isolated embryonic heart (Japanese quail)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Connexin40 Imparts Conduction Heterogeneity to Atrial Tissue. David E. Leaf, Jonathan E. Feig, Carolina Vasquez, Pamela L. Riva, Cindy Yu, Joshua M. Lader, Andrianos Kontogeorgis, Elvera L. Baron, Nicholas S. Peters, Edward A. Fisher, David E. Gutstein and Gregory E. Morley Circ Res. 2008 Jul 3. [Epub ahead of print]	Isolated heart (mouse)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Burst Emergence of Intracellular Ca²⁺ Waves Evokes Arrhythmogenic Oscillatory Depolarization via the Na⁺-Ca²⁺ Exchanger Simultaneous Confocal Recording of Membrane Potential and Intracellular Ca²⁺ in the Heart Katsuji Fujiwara, Hideo Tanaka, Hiroki Mani, Takuo Nakagami and Tetsuro Takamatsu Circ Res. 2008 Aug 29;103(5):509-18. Epub 2008 Jul 17.	Isolated heart (rat)	VSD (RH-237) CaSD (Fluo-4)	MiCAM02
Mechanisms of cardiac arrhythmias and sudden death in transgenic rabbits with long QT syndrome Michael Brunner, Xuwen Peng, Gong Xin Liu, Xiao-Qin Ren, Ohad Ziv, Bum-Rak Choi, Rajesh Mathur, Mohammed Hajjiri, Katja E. Odening, Eric Steinberg, Eduardo J. Folco, Ekatherini Pringa, Jason Centracchio, Roland R. Macharzina, Tammy Donahay, Lorraine Schofield, Naveed Rana, Malcolm Kirk, Gary F. Mitchell, Athena Poppas, Manfred Zehender and Gideon Koren J Clin Invest. 2008 June 2; 118(6): 2246?2259.	Isolated heart (rabbit)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Mechanisms underlying increased right ventricular conduction sensitivity to flecainide challenge Rengasayee Veeraraghavan and Steven Poelzing Cardiovasc Res. 2008 Mar 1;77(4):749-56.	Isolated heart (guinea pig)	VSD (Di-4-ANEPPS)	MiCAM02 (dual camera)
An Experimental Framework to Validate 3D Models of Cardiac Electrophysiology Via Optical Imaging and MRI Mihaela Pop, Maxime Sermesant, Desmond Chung, Garry Liu, Elliot R. McVeigh, Eugene Crystal and Graham A. Wright Lecture Notes in Computer Science Publisher Springer Berlin / Heidelberg Volume 4466/2007; pages 100-109.	Isolated heart (pig)	VSD (Di-4-ANEPPS)	MiCAM02 (dual camera)
Imaging ventricular fibrillation. Guy Salama and Bum-Rak Choi J Electrocardiol. 2007 Nov-Dec;40:S56-61.	Isolated heart (guinea pig)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Spatially discordant voltage alternans cause wavebreaks in ventricular fibrillation. Bum-Rak Choi, Woncheol Jang and Guy Salama Heart Rhythm. 2007 August; 4(8): 1057-1068.	Isolated heart (guinea pig)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Engraftment of connexin 43-expressing cells prevents post-infarct arrhythmia. Wilhelm Roell, Thorsten Lewalter, Philipp Sasse, Yvonne N. Tallini, Bum-Rak Choi, Martin Breitbach, Robert Doran, Ulrich M. Becher, Seong-Min Hwang, Toktam Bostani, Julia von Maltzahn, Andreas Hofmann, Shaun Reining, Britta Eiberger, Bethann Gabris, Alexander Pfeifer, Armin Welz, Klaus Willecke, Guy Salama, Jan W. Schrickel, Michael I. Kotlikoff and Bernd K. Fleischmann Nature. 2007 Dec 6;450(7171):819-24.	In VIvo heart (mouse)	GGCaMP2	MiCAM ULTIMA
Optical Mapping of Langendorff Perfused Human Hearts: Establishing a Model for the Study of Ventricular Fibrillation in Humans. Kumaraswamy Nanthakumar, José Jalife, Stéphane Massé, Eugene Downar, Mihaela Pop, John Asta, Heather Ross, Vivek Rao, Sergey Mironov, Elias Sevaptsidis, Jack Rogers, Graham Wright and Rajesh Dhopeshwarkar Am J Physiol Heart Circ Physiol. 2007 Mar 16;	Isolated heart (guinea pig)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA MiCAM02
Heterogeneous ventricular chamber response to hypokalemia and inward rectifier potassium channel blockade underlies bifurcated T wave in guinea pig. Steven Poelzing and Rengasayee Veeraraghavan Am J Physiol Heart Circ Physiol. 2007 Jun;292(6):H3043-51.	Isolated heart (guinea pig)	VSD (Di-4-ANEPPS)	MiCAM02 (dual camera)
Stereo Reconstruction of the Epicardium for Optical Fluorescence Imaging. Desmond Chung, Mihaela Pop, Maxime Sermesant and Graham A. Wright MICCAI 2006 Workshop on Biophotonics Imaging for Diagnostics and Treatment. 2006 October 6; P33-40.	Isolated heart (pig)	VSD (Di-4-ANEPPS)	MiCAM02 (dual camera)
Pulsatile cardiac tissue grafts using a novel three-dimensional cell sheet manipulation technique functionally integrates with the host heart, in vivo. Akira Furuta, Shunichiro Miyoshi, Yuji Itabashi, Tatsuya Shimizu, Shinichiro Kira, Keiko Hayakawa, Nobuhiro Nishiyama, Kojiro Tanimoto, Yoko Hagiwara, Toshiaki Satoh, Keiichi Fukuda, Teruo Okano and Satoshi Ogawa Circ Res. 2006 Mar 17;98(5):705-12.	Isolated heart Cell sheet (rat)	VSD (Di-4-ANEPPS)	MiCAM01
Interactions between paced wavefronts and monomorphic ventricular tachycardia: implications for antitachycardia pacing. Israel A. Byrd, Matthew W. Kay and Andrew E. Pollard J Cardiovasc Electrophysiol. 2006 Oct;17(10):1129-39.	Isolated heart (rabbit)	VSD (Di-4-ANEPPS)	MiCAM01
Imaging cellular signals in the heart in vivo: Cardiac expression of the high-signal Ca2+ indicator GCaMP2 Yvonne N. Tallini, Masamichi Ohkura, Bum-Rak Choi, Guangju Ji, Keiji Imoto, Robert Doran, Jane Lee, Patricia Plan, Jason Wilson, Hong-Bo Xin, Atsushi Sanbe, James Gulick, John Mathai, Jeffrey Robbins, Guy Salama, Junichi Nakai and Michael I. Kotlikoff Proc Natl Acad Sci USA. 2006 Mar 21;103(12):4753-8. Epub 2006 Mar 13.	In VIvo heart (mouse)	GCaMP2	MiCAM ULTIMA
Properties of new, long-wavelength, voltage-sensitive dyes in the heart. Guy Salama, Bum-Rak Choi, Ghassan Azour, Mitra Lavasani, Vasil Tumbev, Brian M. Salzberg, Michael J. Patrick, Lauren A. Ernst and Alan S. Waggoner J Membr Biol. 2005 Nov;208(2):125-40.	Isolated heart (guinea pig, rabbit, mouse)	VSD (PGH I-VII)	MiCAM ULTIMA
Termination of spiral waves during cardiac fibrillation via shock-induced phase resetting. Richard A Gray and Nipon Chattipakorn PNAS. 2005 Mar 29;102(13):4672-7.	Isolated heart (pig)	VSD (Di-4-ANEPPS)	MiCAM01
Optical Imaging of the Heart. Igor R. Efimov, Vladimir P. Nikolski and Guy Salama. Circulation Research 2004 Jul 9;95(1):21-33.	Isolated heart (guinea pig)	VSD	MiCAM ULTIMA
Three-dimensional surface reconstruction and panoramic optical mapping of large hearts. Matthew W Kay, Philip M Amison and Jack M Rogers IEEE Transactions on Biomedical Engineering, 2004 Jul;51(7):1219-29	Isolated heart (rabbit)	VSD (Di-4-ANEPPS)	MiCAM01
Effect of shock-induced changes in transmembrane potential on reentrant waves and outcome during cardioversion of isolated rabbit hearts. Frederick G. Evans, Raymond E. Ideker and Richard A. Gray J Cardiovasc Electrophysiol. 2002 Nov;13(11):1118-27.	Isolated heart (rabbit)	VSD (Di-4-ANEPPS)	MiCAM01
Effect of Action Potential Duration and Conduction Velocity Restitution and Their Spatial Dispersionon Alternans and the Stability of Arrhythmias. Isabelle Banville and Richard A Gray. J Cardiovasc Electrophysiol. 2002 Nov;13(11):1141-9.	Isolated heart (rabbit)	VSD (Di-4-ANEPPS)	MiCAM01
Anisotropic conduction properties in canine atria analyzed by high-resolution optical mapping: preferential direction of conduction block changes from longitudinal to transverse with increasing age. Takahiro Koura, Motoki Hara, Susumu Takeuchi, Kenichi Ota, Yasumasa Okada, Shunichiro Miyoshi, Atsushi Watanabe, Kazumi Shiraiwa, Hideo Mitamura, Itsuo Kodama and Satoshi Ogawa Circulation. 2002;105:2092	Isolated atrial tissue	VSD (Di-4-ANEPPS)	MiCAM01
Mechanism of Ventricular Defibrillation for Near-Defibrillation Threshold Shocks. Nipon Chattipakorn, Isabelle Banville, Richard A Gray and Raymond E Ideker. Circulation. 2001;104:1313.	Isolated heart (pig)	VSD (Di-4-ANEPPS)	MiCAM01
Spatial and temporal organization during cardiac fibrillation. Richard A. Gray, Arkady M. Pertsov and Jose Jalife. Nature, 392, 75-78 (1998)	Isolated heart (rabbit, sheep)	VSD (Di-4-ANEPPS)	MiCAM01

培養心筋細胞

合計：75

論文名	サンプル	色素	イメージングシステム
Na_V1.5 knockout in iPSCs: a novel approach to study Na_V1.5 variants in a human cardiomyocyte environment. Marion Pierre, Mohammed Djemai, Hugo Poulin, and Mohamed Chahine Sci Rep. 2021; 11: 17168.	Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)	VSD (Di-4-ANEPPS) CaSD (Rhod-2 AM)	MiCAM03
High-Throughput Drug Screening System Based on Human Induced Pluripotent Stem Cell-Derived Atrial Myocytes ∼ A Novel Platform to Detect Cardiac Toxicity for Atrial Arrhythmias. Yayoi Honda, Jun Li, Aya Hino, Shinji Tsujimoto, and Jong-Kook Lee Front Pharmacol. 2021 Aug 3;12:680618.	Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)	VSD (FluoVolt)	MiCAM02
Altered Electrical, Biomolecular, and Immunologic Phenotypes in a Novel Patient-Derived Stem Cell Model of Desmoglein-2 Mutant ARVC. Robert N. Hawthorne, Adriana Blazeski, Justin Lowenthal, Suraj Kannan, Roald Teuben, Deborah DiSilvestre, Justin Morrissette-McAlmon, Jeffrey E. Saffitz, Kenneth R. Boheler, Cynthia A. James, Stephen P. Chelko, Gordon Tomaselli and Leslie Tung J. Clin. Med. 2021, 10(14), 3061;	Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)	VSD (Di-4-ANEPPS) CaSD (Rhod-2 AM)	MiCAM ULTIMA
A 3-D Human Model of Complex Cardiac Arrhythmias. Kenneth Williams, Timothy Liang, Stéphane Massé, Safwat Khan, Rupal Hatkar, Gordon Keller, Kumaraswamy Nanthakumar, Sara S. Nunes Acta Biomaterialia Available online 10 March 2021	Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)	CaSD	MiCAM ULTIMA
Human iPSC engineered cardiac tissue platform faithfully models important cardiac physiology. Willem J. de Lange, Emily T. Farrell, Caroline R. Kreitzer, Derek R. Jacobs, Di Lang, Alexey V. Glukhov, and J. Carter Ralphe Am J Physiol Heart Circ Physiol. 2021 Feb 19.	Human induced pluripotent stem cells (hiPSC-CM)	CaSD	MiCAM ULTIMA
Myocardial repair of bioengineered cardiac patches with decellularized placental scaffold and human-induced pluripotent stem cells in a rat model of myocardial infarction. Yu Jiang, Si-Jia Sun, Zhe Zhen, Rui Wei, Nannan Zhang, Song-Yan Liao & Hung-Fat Tse Stem Cell Res Ther 12, 13 (2021).	Bioengineered cardiac patch (human-induced pluripotent stem cell-derived cardiomyocytes: hiPSC-CMs)	CaSD (Fura-2 AM)	MiCAM ULTIMA
iPSC-derived cardiomyocytes from patients with myotonic dystrophy type 1 have abnormal ion channel functions and slower conduction velocities. Hugo Poulin, Aurélie Mercier, Mohammed Djemai, Valérie Pouliot, Isabelle Deschenes, Mohamed Boutjdir, Jack Puymirat & Mohamed Chahine Scientific Reports volume 11, Article number: 2500 (2021)	Induced pluripotent stem-derived cardiomyocytes (iPSC-CMs)	VSD (Di-4-ANEPPS)	MiCAM03
Scaffold-Mediated Developmental Effects on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Are Preserved After External Support Removal. Jun Li, Jong-Kook Lee, Keiko Miwa, Yuki Kuramoto, Kiyoshi Masuyama, Hideki Yasutake, Satoki Tomoyama, Hiroyuki Nakanishi, and Yasushi Sakata Front Cell Dev Biol. 2021; 9: 591754.	Human induced pluripotent stem-derived cardiomyocytes (hiPSC-CMs)	VSD (FluoVolt)	MiCAM02
Adverse effects of hydroxychloroquine and azithromycin on contractility and arrhythmogenicity revealed by human engineered cardiac tissues. Andy On-Tik Wong, Bimal Gurung, Wing Sum Wong, Suet Yee Mak, Wan Wai Tse, Chloe M. Li, Deborah K. Lieu, Kevin D. Costa, Ronald A. Li, and Roger J. Hajjar J Mol Cell Cardiol. 2021 Apr; 153: 106?110.	Human pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)	?	MiCAM03
Local hyperactivation of L-type Ca²⁺ channels increases spontaneous Ca²⁺ release activity and cellular hypertrophy in right ventricular myocytes from heart failure rats. Roman Y. Medvedev, Jose L. Sanchez-Alonso, Catherine A. Mansfield, Aleksandra Judina, Alice J. Francis, Christina Pagiatakis, Natalia Trayanova, Alexey V. Glukhov, Michele Miragoli, Giuseppe Faggian & Julia Gorelik Scientific Reports volume 11, Article number: 4840 (2021)	Isolated myocytes	CaSD (Fluo-4 AM)	MiCAM ULTIMA
Human Cardiac Fibroblast Number and Activation State Modulate Electromechanical Function of hiPSC-Cardiomyocytes in Engineered Myocardium. Cassady E. Rupert, Tae Yun Kim, Bum-Rak Choi, and Kareen L. K. Coulombe Stem Cells Int. 2020; 2020: 9363809.	Engineered cardiac tissues	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Heart slice culture system reliably demonstrates clinical drug-related cardiotoxicity. Jessica M. Miller, Moustafa H. Meki, Qinghui Oua, Sharon A. George, Anna Gams, Riham R.E. Abouleisa, Xian-Liang Tang, Brooke M. Ahern, Guruprasad A. Giridharan, Ayman El-Baz, Bradford G. Hill, Jonathan Satin, Daniel J.Conklin, Javid Moslehi, Roberto Bolli, Alexandre J.S. Ribeiro, Igor R. Efimov, Tamer M.A. Mohamed Toxicology and Applied Pharmacology, Volume 406, 2020,115213	Cultured heart slice	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Aligned human cardiac syncytium for in vitro analysis of electrical, structural, and mechanical readouts. B. N. Napiwocki, D. Lang, A. Stempien, J. Zhang, R. Vaidyanathan, J. C. Makielski, L. L. Eckhardt, A. V. Glukhov, T. J. Kamp, W. C. Crone Biotechnology and Bioengineering. 2021; 118: 442? 452.	Human pluripotent stem cell derived cardiomyocytes (hPSC-CMs)	CaSD (Rhod-2 AM)	MiCAM ULTIMA
The proarrhythmic features of pathological cardiac hypertrophy in neonatal rat ventricular cardiomyocyte cultures Zeinab Neshati, Martin J Schalij, Antoine A F de Vries J Appl Physiol (1985). 2020 Mar 1;128(3):545-553.	Neonatal rat ventricular cardiomyocytes	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Adrenoceptor Responses in Human Embryonic Stem Cell-Derived Cardiomyocytes: a Special Focus on Electrophysiological Property. Weiwei Jiang, Xingjian Hu, Fei Li, Geng Li, Yin Wang J Pharmacol Exp Ther. 2020 Jun;373(3):429-437.	Human embryonic stem cell]derived cardiomyocytes (hESC-CMs)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Single]Cell Transcriptomics of Engineered Cardiac Tissues From Patient]Specific Induced Pluripotent Stem Cell?Derived Cardiomyocytes Reveals Abnormal Developmental Trajectory and Intrinsic Contractile Defects in Hypoplastic Right Heart Syndrome. Yin]Yu Lam, Wendy Keung, Chun]Ho Chan, Lin Geng, Nicodemus Wong, David Brenière]Letuffe, Ronald A. Li and Yiu]Fai Cheung J Am Heart Assoc. 2020 Oct 20; 9(20): e016528.	Human cardiac anisotropic sheet	VSD (Di-8-ANEPPS)	MiCAM ULTIMA
Combinatorial Treatment of Human Cardiac Engineered Tissues With Biomimetic Cues Induces Functional Maturation as Revealed by Optical Mapping of Action Potentials and Calcium Transients. Andy On-Tik Wong, Nicodemus Wong, Lin Geng, Maggie Zi-ying Chow, Eugene K. Lee, Hongkai Wu, Michelle Khine, Chi-Wing Kong, Kevin D. Costa, Wendy Keung, Yiu-Fai Cheung, and Ronald A. Li Front Physiol. 2020; 11: 165.	Human ventricular cardiac anisotropic sheet preparations	VSD (Di-8-ANEPPS)	MiCAM ULTIMA
Functional Properties of Engineered Heart Slices Incorporating Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Adriana Blazeski, Justin Lowenthal, Renjun Zhu, Jourdan Ewoldt, Kenneth R. Boheler, and Leslie Tung Theranostics. 2019; 9(24): 7222?7238.	Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Generation of human induced pluripotent stem cell-derived cardiomyocytes in 2D monolayer and scalable 3D suspension bioreactor cultures with reduced batch-to-batch variation. Sarkawt Hamad, Daniel Derichsweiler, Symeon Papadopoulos, Filomain Nguemo, Tomo Šarić, Agapios Sachinidis, Konrad Brockmeier, Jürgen Hescheler, Bastiaan J Boukens, and Kurt Pfannkuche Theranostics. 2019; 9(24): 7222?7238.	Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Engineered Heart Slice Model of Arrhythmogenic Cardiomyopathy Using Plakophilin-2 Mutant Myocytes. Adriana Blazeski, Justin Lowenthal, Yin Wang, Roald Teuben, Renjun Zhu, Sharon Gerecht, Gordon Tomaselli, Leslie Tung Tissue Eng Part A. 2019 May;25(9-10):725-735.	Engineered heart slices	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Correlation between frataxin expression and contractility revealed by in vitro Friedreich's ataxia cardiac tissue models engineered from human pluripotent stem cells. Andy On-Tik Wong, Gabriel Wong, Michael Shen, Maggie Zi-Ying Chow, Wan Wai Tse, Bimal Gurung, Suet Yee Mak, Deborah K Lieu, Kevin D Costa, Camie W Chan, Alain Martelli, Joseph F Nabhan, Ronald A Li Stem Cell Res Ther. 2019 Jul 8;10(1):203.	Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs)	VSD (Di-8-ANEPPS)	MiCAM ULTIMA
Contribution of potassium channels to action potential repolarization of human embryonic stem cell-derived cardiomyocytes. Yin Wang, Renjun Zhu, Leslie Tung Br J Pharmacol. 2019 Aug;176(15):2780-2794.	Human embryonic stem cell]derived cardiomyocytes (hESC-CMs)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Enhancement of human iPSC-derived cardiomyocyte maturation by chemical conditioning in a 3D environment. Chen Yu Huang, Rebeca Peres Moreno Maia-Joca, Chin Siang Ong, Ijala Wilson, Deborah DiSilvestre, Gordon F Tomaselli, Daniel H Reich J Mol Cell Cardiol. 2020 Jan;138:1-11.	Human induced pluripotent stem-derived cardiomyocytes (hiPSC-CMs)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Geometrical Patterning and Constituent Cell Heterogeneity Facilitate Electrical Conduction Disturbances in a Human Induced Pluripotent Stem Cell-Based Platform: An In vitro Disease Model of Atrial Arrhythmias. Hiroyuki Nakanishi, Jong-Kook Lee, Keiko Miwa, Kiyoshi Masuyama, Hideki Yasutake, Jun Li, Satoki Tomoyama, Yayoi Honda, Jiro Deguchi, Shinji Tsujimoto, Kyoko Hidaka, Shigeru Miyagawa, Yoshiki Sawa, Issei Komuro, Yasushi Sakata Front Physiol. 2019 Jun 27;10:818.	Human induced pluripotent stem-derived cardiomyocytes (hiPSC-CMs)	VSD (FluoVolt)	MiCAM02
Multiparametric slice culture platform for the investigation of human cardiac tissue physiology. Yun Qiao, Quan Dong, Baichen Li, Sofian Obaid, Christian Miccile, Rose T Yin, Trisha Talapatra, Zexu Lin, Sihui Li, Zhenyu Li, Igor R Efimov Prog Biophys Mol Biol. 2019 Jul;144:139-150.	Cultured murine atria and human slices	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Cardiomyocyte Progenitor Cells as a Functional Gene Delivery Vehicle for Long-Term Biological Pacing. Anna M. D. Végh, A. Dénise Den Haan, Lucía Cócera Ortega, Arie O. Verkerk, Joost P. G. Sluijter, Diane Bakker, Shirley Van Amersfoorth, Toon A. B. Van Veen, Mischa Klerk, Jurgen Seppen, Jacques M. T. De Bakker, Vincent M. Christoffels, Dirk Geerts, Marie José T. H. Goumans, Hanno L. Tan and Gerard J. J. Boink Molecules. 2019 Jan 5;24(1). pii: E181.	Organ explant cultures	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Engineered Heart Slice Model of Arrhythmogenic Cardiomyopathy using Plakophilin-2 Mutant Myocytes. Adriana Blazeski, Justin Lowenthal, Yin Wang, Roald Teuben, Renjun ZhuProf, Sharon Gerecht, Gordon Tomaselli and Leslie Tung Tissue Eng Part A. 2018 Dec 6.	Engineered heart slices	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Optogenetics enables real-time spatiotemporal control over spiral wave dynamics in an excitable cardiac system. Rupamanjari Majumder, Iolanda Feola, Alexander S Teplenin, Antoine AF de Vries, Alexander V Panfilov and Daniel A Pijnappels Elife. 2018 Sep 27;7. pii: e41076.	Neonatal rat atrial cardiomyocytes	VSD (Di-4-ANBDQBS)	MiCAM ULTIMA
A tissue-engineered scale model of the heart ventricle. Luke A. MacQueen, Sean P. Sheehy, Christophe O. Chantre, John F. Zimmerman, Francesco S. Pasqualini, Xujie Liu, Josue A. Goss, Patrick H. Campbell, Grant M. Gonzalez, Sung-Jin Park, Andrew K. Capulli, John P. Ferrier, T. Fettah Kosar, L. Mahadevan, William T. Pu and Kevin Kit Parker Nature Biomedical Engineering (2018). Published: 23 July 2018	Neonatal rat ventricular myocytes	CaSD (Rhod-2)	MiCAM ULTIMA
Multiparametric slice culture platform for the investigation of human cardiac tissue physiology. Yun Qiao, Quan Dong, Baichen Li, Sofian Obaid, Christian Miccile, Rose T. Yin, Trisha Talapatra, Zexu Lin, Sihui Li, Zhenyu Li, and Igor R. Efimov Prog Biophys Mol Biol. 2018 Jun 27. pii: S0079-6107(18)30012-9.	Cultured mouse atrial tissue and human cardiac slices	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Paradoxical Onset of Arrhythmic Waves from Depolarized Areas in Cardiac Tissue Due to Curvature-Dependent Instability. Alexander S. Teplenin, Hans Dierckx, Antoine A. F. de Vries, Daniël A. Pijnappels, and Alexander V. Panfilov Phys Rev X. 2018 Jun 26; 8(2): 021077.	Neonatal rat ventricular myocytes	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Bioengineering an electro-mechanically functional miniature ventricular heart chamber from human pluripotent stem cells. Ronald A.Li, Wendy Keung, Timothy J. Cashman, Peter C. Backeris, Bryce V.Johnson, Evan S.Bardot, Andy O.T. Wong, Patrick K.W. Chan, Camie W.Y. Chan, Kevin D. Costa Biomaterials. 2018 May;163:116-127	Human ventricle-like cardiac organoid chamber	VSD (Di-8-ANEPPS) CaSD (X-rhod-1 AM)	MiCAM ULTIMA
Contractile force measurement of human induced pluripotent stem cell-derived cardiac cell sheet-tissue. Daisuke Sasaki, Katsuhisa Matsuura, Hiroyoshi Seta, Yuji Haraguchi, Teruo Okano, Tatsuya Shimizu PLoS One. 2018 May 23;13(5):e0198026.	Human induced pluripotent stem-derived cardiomyocytes (hiPSC-CMs)	CaSD (Fluo-8)	MiCAM ULTIMA
Resolving Myocardial Activation With Novel Omnipolar Electrograms. Stéphane Massé, Karl Magtibay, Nicholas Jackson, John Asta, Marjan Kusha, Boyang Zhang, Ram Balachandran, Milica Radisic, D. Curtis Deno, and Kumaraswamy Nanthakumar Circ Arrhythm Electrophysiol. 2016 Jul;9(7):e004107. doi: 10.1161/CIRCEP.116.004107.	Monolayers	CaSD (Fluo-4 AM)	MiCAM ULTIMA
Variability of Action Potentials Within and Among Cardiac Cell Clusters Derived from Human Embryonic Stem Cells. Renjun Zhu, Michal A. Millrod, Elias T. Zambidis, and Leslie Tung Sci Rep. 2016 Jan 5;6:18544. doi: 10.1038/srep18544.	Human embryonic stem cell-derived cardiomyocytes (hESC-CMs)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing. Johan U. Lind, Travis A. Busbee, Alexander D. Valentine, Francesco S. Pasqualini, Hongyan Yuan, Moran Yadid, Sung-Jin Park, Arda Kotikian, Alexander P. Nesmith, Patrick H. Campbell, Joost J. Vlassak, Jennifer A. Lewis and Kevin K. Parker Nat Mater. 2016 Oct 24. doi: 10.1038/nmat4782. [Epub ahead of print]	Neonatal rat ventricular myocytes	VSD (RH-237)	MiCAM ULTIMA
Optogenetic Engineering of Atrial Cardiomyocytes. Iolanda Feola, Alexander Teplenin, Antoine A. F. de Vries, Daniël A. Pijnappels Methods Mol Biol. 2016;1408:319-31. doi: 10.1007/978-1-4939-3512-3_22.	Atrial cardiomyocytes (aCMCs)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
A Micropatterned Human Pluripotent Stem Cell-Based Ventricular Cardiac Anisotropic Sheet for Visualizing Drug-Induced Arrhythmogenicity. Angie M. Y. Shum, Hui Che, Andy On-Tik Wong, Chenzi Zhang, Hongkai Wu, Camie W. Y. Chan, Kevin Costa, Michelle Khine, Chi-Wing Kong, Ronald A. Li Adv Mater. 2016 Nov 2. doi: 10.1002/adma.201602448. [Epub ahead of print]	Human ventricular cardiac anisotropic sheet (hvCAS)	VSD (Di-8-ANEPPS)	MiCAM ULTIMA
Islands of spatially discordant APD alternans underlie arrhythmogenesis by promoting electrotonic dyssynchrony in models of fibrotic rat ventricular myocardium Rupamanjari Majumder, Marc C. Engels, Antoine A. F. de Vries, Alexander V. Panfilov, and Daniël A. Pijnappels Sci Rep. 2016; 6: 24334. Published online 2016 Apr 13. doi: 10.1038/srep24334	Monolayers	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Electroactive polyurethane/siloxane derived from castor oil as a versatile cardiac patch, Part II: HL-1 cytocompatibility and electrical characterizations. Nafiseh Baheiraei, Reza Gharibi, Hamid Yeganeh, Michele Miragoli, Nicolò Salvarani, Elisa Di Pasquale and Gianluigi Condorelli J Biomed Mater Res A. 2016 Jan 28. doi: 10.1002/jbm.a.35669. [Epub ahead of print]	HL-1 monolayers	CaSD (Fluo-4 AM)	MiCAM ULTIMA
The protective effect of ursodeoxycholic acid in an in vitro model of the human fetal heart occurs via targeting cardiac fibroblasts. Francisca Schultz, Alveera Hasan, Anita Alvarez-Laviada, Michele Miragoli, Navneet Bhogal, Sarah Wells, Claire Poulet, Jenny Chambers, Catherine Williamson and Julia Gorelik Prog Biophys Mol Biol. 2016 Jan 8. pii: S0079-6107(16)00005-5. doi: 10.1016/j.pbiomolbio.2016.01.003. [Epub ahead of print]	Isolated cardiomyocytes	CaSD (Fluo-4 AM) VSD (Di-8-ANEPPS)	MiCAM ULTIMA
Microtubule-Dependent Mitochondria Alignment Regulates Calcium Release in Response to Nanomechanical Stimulus in Heart Myocytes. Michele Miragoli, Jose L. Sanchez-Alonso, Anamika Bhargava, Peter T. Wright, Markus Sikkel, Sophie Schobesberger, Ivan Diakonov, Pavel Novak, Alessandra Castaldi, Paola Cattaneo, Alexander R. Lyon, Max J. Lab, Julia Gorelik Cell Rep. 2016 Jan 5;14(1):140-51. doi: 10.1016/j.celrep.2015.12.014. Epub 2015 Dec 24.	Isolated cardiomyocytes	CaSD (Fluo-4 AM)	MiCAM ULTIMA
Modeling and study of the mechanism of dilated cardiomyopathy using induced pluripotent stem cells derived from individuals with Duchenne muscular dystrophy. Bo Lin, Yang Li, Lu Han, Aaron D. Kaplan, Ying Ao, Spandan Kalra, Glenna C. L. Bett, Randall L. Rasmusson, Chris Denning and Lei Yang Dis Model Mech. 2015 May;8(5):457-66. doi: 10.1242/dmm.019505. Epub 2015 Mar 19.	Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs)	CaSD (Rhod-2 AM)	MiCAM ULTIMA
Direct Evidence for Microdomain-Specific Localization and Remodeling of Functional L-Type Calcium Channels in Rat and Human Atrial Myocytes. Alexey V. Glukhov, Marina Balycheva, Jose L. Sanchez-Alonso, Zeki Ilkan, Anita Alvarez-Laviada, Navneet Bhogal, Ivan Diakonov, Sophie Schobesberger, Markus B. Sikkel, Anamika Bhargava, Giuseppe Faggian, Prakash P. Punjabi, Steven R. Houser and Julia Gorelik Circulation. 2015 Oct 8. pii: CIRCULATIONAHA.115.018131. [Epub ahead of print]	Human atrial myocytes	CaSD (Fluo-4 AM)	MiCAM ULTIMA
Phospholamban as a crucial determinant of the inotropic response of human pluripotent stem cell-derived ventricular cardiomyocytes and engineered 3-dimensional tissue constructs. Gaopeng Chen, Sen Li, Ioannis Karakikes, Lihuan Ren, Maggie Zi-Ying Chow, Anant Chopra, Wendy Keung, Bin Yan, Camie WY Chan, Kevin D. Costa, Chi-Wing Kong, Roger J. Hajjar, Christopher S. Chen and Ronald A. Li Circ Arrhythm Electrophysiol. 2015 Feb;8(1):193-202. doi: 10.1161/CIRCEP.114.002049. Epub 2014 Dec 10.	hESC/iPSC-vCMs	CaSD (X-Rhod-1 AM)	MiCAM ULTIMA
Combining TGF-ƒÀ signal inhibition and connexin43 silencing for iPSC induction from mouse cardiomyocytes Ping Dai, Yoshinori Harada, Hitoshi Miyachi, Hideo Tanaka, Satsuki Kitano, Tetsuya Adachi, Tomoyuki Suzuki, Hitoshi Hino and Tetsuro Takamatsu Sci Rep. 2014; 4: 7323.	Cardiomyocytes derived from siPSCs	CaSD (Fluo-4 AM)	MiCAM02
Light-induced termination of spiral wave arrhythmias by optogenetic engineering of atrial cardiomyocytes. Brian O. Bingen, Marc C. Engels, Martin J. Schalij, Wanchana Jangsangthong, Zeinab Neshati, Iolanda Feola, Dirk L. Ypey, Saïd F.A. Askar, Alexander V. Panfilov, Daniël A. Pijnappels and Antoine A.F. de Vries Cardiovasc Res. 2014 Jul 31. pii: cvu179. [Epub ahead of print]	Neonatal rat atrial cardiomyocyte monolayers	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Calcium Transients Closely Reflect Prolonged Action Potentials in iPSC Models of Inherited Cardiac Arrhythmia. C. Ian Spencer, Shiro Baba, Kenta Nakamura, Ethan A. Hua, Marie A.F. Sears, Chi-cheng Fu, Jianhua Zhang, Sadguna Balijepalli, Kiichiro Tomoda, Yohei Hayashi, Paweena Lizarraga, Julianne Wojciak, Melvin M. Scheinman, Katriina Aalto-Setälä, Jonathan C. Makielski, Craig T. January, Kevin E. Healy, Timothy J. Kamp, Shinya Yamanaka, Bruce R. Conklinemail Stem Cell Reports. 2014 Aug 12;3(2):269-281.	Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs)	CaSD (Fluo-4 AM)	MiCAM02
Quality metrics for stem cell-derived cardiac myocytes. Sean P. Sheehy, Francesco Pasqualini, Anna Grosberg, Sung Jin Park, Yvonne Aratyn-Schaus and Kevin Kit Parker Stem Cell Reports. 2014 Mar 6;2(3):282-94. doi: 10.1016/j.stemcr.2014.01.015. eCollection 2014.	Neonatal mouse ventricular cardiomyocytes	VSD (RH-237)	MiCAM ULTIMA
Direct Reprogramming of Human Fibroblasts toward a Cardiomyocyte-like State. Weining Bian, Nima Badie, Herman D. Himel IV and Nenad Bursac Biomaterials. 2014 Apr;35(12):3819-28. doi: 10.1016/j.biomaterials.2014.01.045. Epub 2014 Feb 6.	Neonatal rat ventricular cardiomyocytes	CaSD (Rhod-2)	MiCAM ULTIMA
Effect of engineered anisotropy on the susceptibility of human pluripotent stem cell-derived ventricular cardiomyocytes to arrhythmias. Jiaxian Wang, Aaron Chen, Deborah K. Lieu, Ioannis Karakikes, Gaopeng Chen, Wendy Keung, Camie W. Chan, Roger J. Hajjar, Kevin D. Costa, Michelle Khine and Ronald A. Li Biomaterials. 2013 Nov;34(35):8878-86. doi: 10.1016/j.biomaterials.2013.07.039. Epub 2013 Aug 12.	Human pluripotent stem cell-derived ventricular cardiomyocytes (hPSC-CMs)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Direct Reprogramming of Human Fibroblasts toward a Cardiomyocyte-like State. Ji-Dong Fu, Nicole R. Stone, Lei Liu, C. Ian Spencer, Li Qian, Yohei Hayashi, Paul Delgado-Olguin, Sheng Ding, Benoit G. Bruneau and Deepak Srivastava Stem Cell Reports, Volume 1, Issue 3, 235-247, 22 August 2013	Human embryonic stem cell-derived cardiomyocytes (hESC-CMs)	CaSD (Fluo-4 AM)	MiCAM02
CaMKII inhibition rectifies arrhythmic phenotype in a patient-specific model of catecholaminergic polymorphic ventricular tachycardia. Di Pasquale E, Lodola F, Miragoli M, Denegri M, Avelino-Cruz JE, Buonocore M, Nakahama H, Portararo P, Bloise R, Napolitano C, Condorelli G and Priori SG Cell Death Dis. 2013 Oct 10;4:e843. doi: 10.1038/cddis.2013.369.	iPSC-derived cardiac cells	CaSD (Fluo-4 AM)	MiCAM ULTIMA
Repopulation of decellularized mouse heart with human induced pluripotent stem cell-derived cardiovascular progenitor cells. Tung-Ying Lu, Bo Lin, Jong Kim, Mara Sullivan, Kimimasa Tobita, Guy Salama and Lei Yang Nat Commun. 2013 Aug 14;4:2307. doi: 10.1038/ncomms3307.	Human induced pluripotent stem cell-derived cardiovascular progenitor cells	CaSD (Rhod-2)	MiCAM ULTIMA
Biowire: a platform for maturation of human pluripotent stem cell-derived cardiomyocytes. Sara S Nunes, Jason W Miklas, Jie Liu, Roozbeh Aschar-Sobbi, Yun Xiao, Boyang Zhang, Jiahua Jiang, Stéphane Massé, Mark Gagliardi, Anne Hsieh, Nimalan Thavandiran, Michael A Laflamme, Kumaraswamy Nanthakumar, Gil J Gross, Peter H Backx, Gordon Keller and Milica Radisic Nat Methods. 2013 Aug;10(8):781-7. doi: 10.1038/nmeth.2524. Epub 2013 Jun 23.	Human pluripotent stem cell derived cardiomyocytes (hPSC-CMs)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Fibroblast KATP Currents Modulate Myocyte Electrophysiology in Infarcted Hearts. Najate Benamer, Carolina Vasquez, Vanessa M Mahoney, Maximilian J Steinhardt, William A Coetzee, and Gregory E Morley Am J Physiol Heart Circ Physiol. 2013 May;304(9):H1231-9. doi: 10.1152/ajpheart.00878.2012. Epub 2013 Feb 22.	Monolayer	VSD (Di-8-ANEPPS)	MiCAM ULTIMA
Oscillation in Cycle Length Induces Transient Discordant and Steady-State Concordant Alternans in the Heart. Seth H. Weinberg and Leslie Tung PLoS One. 2012;7(7):e40477. Epub 2012 Jul 5.	Monolayer	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
ILK Induces Cardiomyogenesis in the Human Heart. Alexandra Traister, Shabana Aafaqi, Stephane Masse, Xiaojing Dai, Mark Li, Aleksander Hinek, Kumaraswamy Nanthakumar, Gregory Hannigan and John G. Coles Med Image Anal. 2012 Feb;16(2):505-23. Epub 2011 Dec 6.	Human fetal cardiomyocytes	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Functional Scaffold-Free 3D Cardiac Microtissues: a Novel Model for the Investigation of Heart Cells. Bethany R Desroches, Peng Zhang, Bum-Rak Choi, Michelle E King, Angel E Maldonado, Weiyan Li, Adam Rago, Gong-Xin Liu, Nandan Nath, Kathryn M Hartmann, Bryant Yang, Gideon Koren, Jeffrey R Morgan and Ulrike Mende Am J Physiol Heart Circ Physiol. 2012 Mar 16. [Epub ahead of print]	Neonatal rat cardiomyocytes	VSD (RH-237)	MiCAM ULTIMA
High-purity enrichment of functional cardiovascular cells from human iPS cells. Bo Lin, Jong Kim, Yanxin Li, Haiying Pan, Xonia Carvajal-Vergara, Guy Salama, Tao Cheng, Yong Li, Cecilia W. Lo and Lei Yang Cardiovasc Res. 2012 Aug 1;95(3):327-35. Epub 2012 Jun 6.	Human iPSC-derived cardiovascular cells	CaSD (Rhod-2)	MiCAM ULTIMA
Connexin43 silencing in myofibroblasts prevents arrhythmias in myocardial cultures: role of maximal diastolic potential. Saïd F. Askar, Brian O. Bingen, Jim Swildens, Dirk L. Ypey, Arnoud van der Laarse, Douwe E. Atsma, Katja Zeppenfeld, Martin J. Schalij, Antoine A. de Vries and Daniël A. Pijnappels Cardiovasc Res. 2012 Mar 1;93(3):434-44. Epub 2011 Dec 22.	Primary ventricular cardiomyocytes	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Shrink-film configurable multiscale wrinkles for functional alignment of human embryonic stem cells and their cardiac derivatives. Aaron Chen, Deborah K. Lieu, Lauren Freschauf, Valerie Lew, Himanshu Sharma, Jiaxian Wang, Diep Nguyen, Ioannis Karakikes, Roger J. Hajjar, Ajay Gopinathan, Elliot Botvinick, Charless C. Fowlkes, Ronald A. Li and Michelle Khine Adv Mater. 2011 Dec 22;23(48):5785-91. doi: 10.1002/adma.201103463. Epub 2011 Nov 8.	Human embryonic stem cells-derived cardiomyocytes (hESC-CMs)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Human embryonic and fetal mesenchymal stem cells differentiate toward three different cardiac lineages in contrast to their adult counterparts. Arti A. Ramkisoensing, Daniël A. Pijnappels, Saïd F. A. Askar, Robert Passier, Jim Swildens, Marie José Goumans, Cindy I. Schutte, Antoine A. F. de Vries, Sicco Scherjon, Christine L. Mummery, Martin J. Schalij and Douwe E. Atsma PLoS One. 2011;6(9):e24164. Epub 2011 Sep 9.	Neonatal rat ventricular cardiomyocytes	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
A protective antiarrhythmic role of ursodeoxycholic acid in an in vitro rat model of the cholestatic fetal heart. Michele Miragoli, Siti H. Sheikh Abdul Kadir, Mary N. Sheppard, Nicoló Salvarani, Matilda Virta, Sarah Wells, Max J. Lab, Viacheslav O. Nikolaev, Alexey Moshkov, William M. Hague, Stephan Rohr, Catherine Williamson and Julia Gorelik Hepatology. 2011 Oct;54(4):1282-92. doi: 10.1002/hep.24492. Epub 2011 Aug 1.	Monolayer	VSD (Di-8-ANEPPS)	MiCAM ULTIMA
Antiproliferative treatment of myofibroblasts prevents arrhythmias in vitro by limiting myofibroblast-induced depolarization. Saïd F.A. Askar, Arti A. Ramkisoensing, Martin J. Schalij, Brian O. Bingen, Jim Swildens, Arnoud van der Laarse, Douwe E. Atsma, Antoine A.F. de Vries, Dirk L. Ypey and Daniël A. Pijnappels Cardiovasc Res. 2011 Feb 2. [Epub ahead of print]	Neonatal rat ventricular cardiomyocytes	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Enhanced fibroblast-myocyte interactions in response to cardiac injury. Carolina Vasquez, Poornima Mohandas, Karen L. Louie, Najate Benamer, Ashwini C. Bapat and Gregory E. Morley Circ Res. 2010 Oct 15;107(8):1011-20. Epub 2010 Aug 12.	Monolayer	VSD (Di-8-ANEPPS)	MiCAM ULTIMA
Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors. Masaki Ieda, Ji-Dong Fu, Paul Delgado-Olguin, Vasanth Vedantham, Yohei Hayashi, Benoit G. Bruneau and Deepak Srivastava Cell. 2010 Aug 6;142(3):375-86.	Cardiac fibroblast?derived induced cardiomyocytes	CaSD (Rhod-3)	MiCAM02
Bile Acid-Induced Arrhythmia Is Mediated by Muscarinic M₂ Receptors in Neonatal Rat Cardiomyocytes. Siti H. Sheikh Abdul Kadir, Michele Miragoli, Shadi Abu-Hayyeh, Alexey V. Moshkov, Qilian Xie, Verna Keitel, Viacheslav O. Nikolaev, Catherine Williamson and Julia Gorelik PLoS One. 2010; 5(3): e9689.	Ventricular neonatal rat myocyte	CaSD (Fluo-4 AM)	MiCAM ULTIMA
Engineered heart tissue: a novel tool to study the ischemic changes of the heart in vitro. Rajesh G. Katare, Motonori Ando, Yoshihiko Kakinuma and Takayuki Sato PLoS One. 2010 Feb 17;5(2):e9275.	Engineered heart tissue	VSD (Di-4-ANEPPS)	MiCAM02
Interrogating functional integration between injected pluripotent stem cell-derived cells and surrogate cardiac tissue. Hannah Song, Charles Yoon, Steven J. Kattman, Jana Dengler, Stéphane Massé, Thushaanthini Thavaratnam, Mena Gewarges, Kumaraswamy Nanthakumar, Michael Rubart, Gordon M. Keller, Milica Radisic and Peter W. Zandstra Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3329-34.	Engineered heart tissue	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Electrogram fractionation in murine HL-1 atrial monolayer model. Karthikeyan Umapathy, Stephane Masse, Karolina Kolodziejska, George D. Veenhuyzen, Vijay S. Chauhan, Mansoor Husain, Talha Farid, Eugene Downar, Elias Sevaptsidis and Kumaraswamy Nanthakumar Heart Rhythm. 2008 Jul;5(7):1029-35.	Monolayer (HL-1)	VSD (Di-4-ANEPPS)	MiCAM ULTIMA
Generation of reentrant arrhythmias by dominant-negative inhibition of connexin43 in rat cultured myocyte monolayers Takuo Nakagami, Hideo Tanaka, Ping Dai, Shien-Fong Lin, Takuji Tanabe, Hiroki Mani, Katsuji Fujiwara, Hiroaki Matsubara and Tetsuro Takamatsu Cardiovasc Res. 2008 Jul 1;79(1):70-9. Epub 2008 Mar 31.	Monolayer	CaSD (Fluo-4)	MiCAM02
Myofibroblasts induce ectopic activity in cardiac tissue. Michele Miragoli, Nicolò Salvarani and Stephan Rohr Circ Res. 2007 Oct 12;101(8):755-8.	Monolayer	VSD (Di-8-ANEPPS)	MiCAM ULTIMA
Dynamic changes of cardiac conduction during rapid pacing. Aleksandar A. Kondratyev, Julien G. C. Ponard, Adelina Munteanu, Stephan Rohr and Jan P. Kucera Am J Physiol Heart Circ Physiol. 2007 Apr;292(4):H1796-1811.	Monolayer	VSD (Di-8-ANEPPS)	MiCAM ULTIMA
A new method for manufacturing cardiac cell sheets using fibrin-coated dishes and its electrophysiological studies by optical mapping. Yuji Itabashi, Shunichiro Miyoshi, Haruko Kawaguchi, Shinsuke Yuasa, Kojiro Tanimoto, Akira Furuta, Tatsuya Shimizu, Teruo Okano, Keiichi Fukuda and Satoshi Ogawa Artif Organs. 2005 Feb;29(2):95-103.	Myocardial cell sheets	VSD (Di-4-ANEPPS)	MiCAM01

膜電位イメージングデータ | 単離心臓 / 培養心筋細胞

sakuraba — Wed, 18 Sep 2024 06:10:31 +0000

ブタ単離心臓の高速イメージング（256x256画素で1,818フレーム/秒）

サンプル	ブタ単離心臓
蛍光色素	膜電位感受性色素
イメージングシステム	MiCAM03-N256
画素数	256x256
フレームレート	1,818fps (0.55msec/frame)
提供	Dr. Jack M. Rogers Department of Biomedical Engineering The University of Alabama at Birmingham

The Nervous Heart: Insights into Autonomic-Mediated Arrhythmias

心房および心室活動電位の高速光学マッピング

サンプル	マウス単離心臓
蛍光色素	膜電位感受性色素
イメージングシステム	MiCAM03-N256
画素数	256x256
フレームレート	1,000fps (1.0msec/frame)

ブタ心臓の心室細動の高速光学マッピング

Sample	Isolated Langendorff Pig Heart
Imaging System	MiCAM02-HR
Provided by	Dr. Mihaela Pop, Sunnybrook Research Institute, Dept Medical Biophysics, University of Toronto

サンプル	ブタ単離心臓
蛍光色素	膜電位感受性色素
イメージングシステム	MiCAM02-HR
提供	Dr. Mihaela Pop, Sunnybrook Research Institute, Dept Medical Biophysics, University of Toronto

モルモット心臓の心室細動の高速光学マッピング

モルモットの心臓の心室細動 (VF)。 VF はバースト刺激 (35 ミリ秒間隔、100 パルス) によって誘発され、視野は心臓の前部領域から正確に 1x1 cm (倍率 1:1) です。このトレースに示すように、波面を検出するために活動電位の一次導関数が計算されました。活動電位の伝播のアニメーションは、波面の方向と曲率の詳細を明確に示しています。心臓を70mmHgの灌流圧および20μlのDi-4-ANEPPS stock solution（1mg/1ml DMSO）で逆行的に灌流した。

提供	Dr.Bum-Rak Choi, Cardiovascular Research Center, Rhode Island Hospital and Brown Medical School

心室細動一次微分アニメーション

モルモットの心臓における活動電位の伝播。心臓はランゲンドルフ灌流され、PGH1 で染色されました。心臓の前面は、空間解像度 0.15x0.15 mm2 の 100x100 CMOS チップに焦点が当てられました。心臓は左側から刺激され、活動電位の伝播が 10,000 フレーム/秒で記録されました。このトレースに示すように、波面を検出するために活動電位の一次導関数が計算されました。

提供	Dr. Guy Salama and Dr.Bum-Rak Choi, Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine

ヒトiPS細胞由来心筋スフェロイドを用いた膜電位とカルシウムの同時マッピング

サンプル	ヒトiPS細胞由来心筋スフェロイド
刺激方法	電気刺激
蛍光プローブ	膜電位感受性色素 (RH237、左画像) カルシウム感受性蛍光タンパク質 (jRCaMP1b、右画像)
イメージングシステム	MiCAM03-N256 2カメラシステム
画素数	256x256
フレームレート	500fps (2.0msec/frame)
提供	Dr. Hanyu Zhang, Dr. Bijay Guragain. Dr. Jianyi Zhang, and Dr. Jack M. Rogers Department of Biomedical Engineering The University of Alabama at Birmingham
参考論文	Optogenetic stimulation and simultaneous optical mapping of membrane potential and calcium transients in human engineered cardiac spheroids Journal of Molecular and Cellular Cardiology 199 (2025) 51–59

ヒトiPS細胞由来心筋細胞の膜電位オプティカルマッピング

サンプル	ヒトiPS細胞由来心筋細胞 (hiPSC-CMs)
蛍光プローブ	膜電位感受性色素 (FluoVolt)
イメージングシステム	MiCAM05-Ultima
画素数	100x100
フレームレート	200fps (5.0msec/frame)
提供	Dr. Hee Jae Jang, Dr. Vladislav Leonov and Dr. Alexey Glukhov University of Wisconsin-Madison

培養心筋細胞のカルシウムイメージング例

撮像装置	MiCAM-C35IR 解像度： 636x360画素撮像速度： 200fps カメラゲイン： 16ｘ
光学系	倒立マクロ蛍光顕微鏡対物レンズ： 5x 結像レンズ： 135mm/2.0 LED光源： LEX2-B（12.5％出力）
その他	多点電極： MED64（アルファメッドサイエンティフィック社製）ペーシングおよび細胞外電位の同時記録ステージトップインキュベータ（東海ヒット社製）色素： Cal-520（AAT bioquest社製）
ご提供	東邦大学医学部医学科薬理学講座中瀬古（泉）寛子先生より
備考	弱い励起光でもシグナルが得られるため、20秒～30秒間の撮像でもベースラインのドリフトは小さく、何度も撮像が可能だった。

マウスの心房心筋細胞を用いた高速カルシウムイメージング

サンプル	新たに単離されたマウス心房心筋細胞。細胞はラミニンでコーティングされたカバーガラス上に播種され、単離と同じ日に使用されました。
方法	細胞は灌流溶液を通して印加される電場によってペーシングされました。イメージングは、20X レンズと SPECTRA-X Lumencor 光源を備えた Nikon DIAPHOT 300 顕微鏡で実行されました。
蛍光色素	カルシウム色素 (Fluo-4 AM)
イメージングシステム	MiCAM05-ULTIMA
画素数	256x256
フレームレート	200fps (5.0msec/frame)
提供	Dr. Roman Medvedev and Dr. Alexey Glukhov, Department of Medicine, University of Wisconsin-Madison

膜電位感受性色素で染色した新生児マウス心室筋細胞の単層

サンプル	新生マウス心室筋細胞の単層。サンプルの 3 か所 (蛍光写真で確認できる) を針先で引っ掻き、伝導ブロックと波面の蛇行を引き起こしました。
方法	サンプルの端に配置されたガラス微小電極を使用して、サンプルをさまざまな周波数で局所的に電気刺激
蛍光色素	膜電位感受性色素 (Di-8-Anepps)
イメージングシステム	MiCAM03-N256
画素数	256x256
フレームレート	1,000fps (1.0msec/frame)
提供	Dr. Jan Kucera and Dr. Ange Maguy, Department of Physiology, University of Bern

培養心筋細胞のカルシウムイメージング (Fluo-4)

培養ラット心筋細胞をFluo-4で染色し、2002年にMiCAM01（旧モデル）を用いて活動を記録しました。現行モデルMiCAM02では、空間分解能、感度、S/N比の点でデータが大幅に向上しました。

培養新生ラット心室細胞の電位感受性色素イメージング

Sustained spiral wave reentry in a 3 day old monolayer disc of cultured neonatal rat ventricular cardiomyocytes (frequency ~ 5 Hz; diameter of the preparation = 8 mm). The preparation was stained with the voltage sensitive dye di-8-ANEPPS and activity was recorded at 1,000 frames/second using a custom-made tandem lens macroscope and an Ultima-L camera system.
培養新生ラット心室細胞の生後 3 日後の単層ディスクにおける持続的なスパイラル・リエントリー (周波数 ~ 5 Hz、標本の直径 = 8 mm)。標本を電位感受性色素 di-8-ANEPPS で染色し、カスタムメイドのタンデムレンズマクロスコープと Ultima-L カメラシステムを使用して 1,000 フレーム/秒で活動を記録しました。

提供	Dr.Stephan Rohr, University of Bern

ブレインビジョン株式会社

ヒトiPS細胞由来 心筋スフェロイドを用いた膜電位とカルシウムの同時マッピング

2Dから3Dへ：より生体に近い心臓モデルでの研究を

2D培養モデルの欠点

心筋スフェロイド：3Dがもたらす高い生理学的妥当性

心筋スフェロイドを使うメリット

革新的な「オールオプティカル」電気生理学プラットフォーム

「オールオプティカル」システムがもたらすメリット

サンプル動画

3Dスフェロイドだからこそ可能な高度な解析

スフェロイドを使ったオールオプティカルシステムがもたらすメリット

参考商品

主なアプリケーション

構成と特長

高速カメラ

ソフトウェア

マクロ蛍光顕微鏡

高輝度LED光源

多機能電気刺激装置

実績

お問い合わせ

第72回日本実験動物学会総会にて機器展示を行います。

中国Beijing Yeeran Technology製小動物用 超音波イメージング装置の製品ページをアップしました

内因性信号イメージングデータと論文一覧

マウス In Vivo脳 内因性信号イメージング (MiCAM ULTIMA-H)

内因性信号イメージング（Intrinsic Optical Signal Imaging）

フラビン蛋白蛍光イメージング

GCaMPイメージングデータと論文一覧

In Vivo GCaMPイメージング (MiCAM02-HR)

論文一覧

テクニカルガイド | MiCAMにおける膜電位光測定のノイズに関する考察と可能性

テクニカルガイド | 神経活動を光で追う

1. 神経活動の観察手段としての膜電位感受性色素の特徴

2. 計測の問題点―ノイズと信号

経験的に見出された有効な染色法

3. 明るい光学系と高速カメラシステム

4. 計測結果

おわりに

テクニカルガイド | ランゲンドルフ灌流心の膜電位光学計測

参考動画

論文一覧 | 単離心臓・培養心筋細胞 | 膜電位感受性色素

単離心臓（ランゲンドルフ灌流心）

培養心筋細胞

膜電位イメージングデータ | 単離心臓 / 培養心筋細胞

ブタ単離心臓の高速イメージング（256x256画素で1,818フレーム/秒）

The Nervous Heart: Insights into Autonomic-Mediated Arrhythmias

心房および心室活動電位の高速光学マッピング

ブタ心臓の心室細動の高速光学マッピング

モルモット心臓の心室細動の高速光学マッピング

心室細動 一次微分アニメーション

ヒトiPS細胞由来 心筋スフェロイドを用いた膜電位とカルシウムの同時マッピング

ヒトiPS細胞由来心筋細胞の膜電位オプティカルマッピング

培養心筋細胞のカルシウムイメージング例

マウスの心房心筋細胞を用いた高速カルシウムイメージング

膜電位感受性色素で染色した新生児マウス心室筋細胞の単層

培養心筋細胞のカルシウムイメージング (Fluo-4)

培養新生ラット心室細胞の電位感受性色素イメージング

ヒトiPS細胞由来心筋スフェロイドを用いた膜電位とカルシウムの同時マッピング

中国Beijing Yeeran Technology製小動物用超音波イメージング装置の製品ページをアップしました

マウス In Vivo脳　内因性信号イメージング (MiCAM ULTIMA-H)

心室細動一次微分アニメーション

ヒトiPS細胞由来心筋スフェロイドを用いた膜電位とカルシウムの同時マッピング