膜電位感受性色素の一覧

一般的に使用されている膜電位感受性色素(Voltage Sensitive Dye)をまとめました。

色素名波長参考論文備考メーカー
Di-4-ANEPPS
(JPW211)
Ex: 530nm
Em: 590nm
Spectra, membrane binding, and potentiometric responses of new charge shift probes
Biochemistry . 1985 Oct 8;24(21):5749-55.

Functional Dissection of Ipsilateral and Contralateral Neural Activity Propagation Using Voltage-Sensitive Dye Imaging in Mouse Prefrontal Cortex
eNeuro 17 November 2023, 10 (12) ENEURO.0161-23.2023;
- Thermo Fischer Scientific

- Biotium

- Santa Cruz Biotechnology

- Potentiometric Probes

- AnaSpec
Di-2-ANEPEQ
(JPW1114)
Ex: 530nm
Em: 590nm
Optical signals from neurons with internally applied voltage-sensitive dyes
J Neurosci . 1995 Feb;15(2):1392-405.
Designed for intracellular application. Also, work well when applied externally, but may wash out quickly.- Potentiometric Probes

- Thermo Fischer Scientific
Di-8-ANEPPS
(JPW1153)
Ex: 530nm
Em: 590nm
Localized membrane depolarizations and localized calcium influx during electric field-guided neurite growth
Neuron . 1992 Sep;9(3):393-403.
Very slow internalization, permitting studies with longer durations.

Dual wavelength ratiometric fluorescence (Ex:450nm/510 nm, Em>570nm) can be used.
- Potentiometric Probes

- Thermo Fischer Scientific

- AAT Bioquest
Di-4-ANBDQBS
(JPW6003)
Ex: 650nm
Em: 720nm
Near Infrared Voltage Sensitive Fluorescent Dyes Optimized for Optical Mapping in Blood-Perfused Myocardium
Heart Rhythm. 2007 Nov; 4(11): 1441-1451.
- Potentiometric Probes

- AAT Bioquest
RH-237Ex: 528nm
Em: 782nm
Improved fluorescent probes for the measurement of rapid changes in membrane potential.
Biophys J. 1982 Sep; 39(3): 301-308.
- Thermo Fischer Scientific

- AAT Bioquest
RH-421Ex: 515nm
Em: 704nm
Fluorescence monitoring of electrical responses from small neurons and their processes.
Biophys J. 1983 May; 42(2): 195-198.
- MedChemExpress

- Santa Cruz Biotechnology
RH-795Ex: 530nm
Em: 712nm
Cortical point-spread function and long-range lateral interactions revealed by real-time optical imaging of macaque monkey primary visual cortex.
J Neurosci. 1994 May 1; 14(5): 2545-2568.
- MedChemExpress

- Biotium

- AAT Bioquest
RH-414Ex: 532nm
Em: 716nm
Real-time optical imaging of naturally evoked electrical activity in intact frog brain.
Nature volume 308, pages848-850 (1984)
- Biotium

- MedChemExpress

- AAT Bioquest
RH-1691Ex: 630nm
Em: 700nm
Long-term voltage-sensitive dye imaging reveals cortical dynamics in behaving monkeys.
J Neurophysiol . 2002 Dec;88(6):3421-38.
Not commercially available
RH-1692Ex: 630nm
Em: 700nm
Imaging cortical dynamics at high spatial and temporal resolution with novel blue voltage-sensitive dyes.
Neuron . 1999 Dec;24(4):791-802.
Not commercially available
FluoVoltEx: 488nm
Em: 517nm
Geometrical Patterning and Constituent Cell Heterogeneity Facilitate Electrical Conduction Disturbances in a Human Induced Pluripotent Stem Cell-Based Platform: An In vitro Disease Model of Atrial Arrhythmias.
Front Physiol. 2019; 10: 818.
- Thermo Fischer Scientific
ANNINE-6plusEx: 488nm
Em: 600nm
ANNINE-6plus, a voltage-sensitive dye with good solubility, strong membrane binding and high sensitivity
Eur Biophys J. 2008 Apr; 37(4): 509-514.
ANNINE-6plus has a fractional fluorescent intensity change (ΔF/F per 100 mV change) of about 30% with single-photon excitation (~488 nm) and >50% with two-photon excitation (~1060 nm).Not commercially available
PGH I-VIII
(Pittsburgh I-VIII)
Ex: 550nm / Em: 720nm
Ex: 670nm / Em: 830nm
Properties of New, Long Wavelength, Voltage-Sensitive Dyes in the Heart
J Membr Biol. 2005 Nov; 208(2): 125-140. 509-514.
2 excitation and 2 voltage-sensitive emission peaks, with large Stokes shifts (> 100 nm). Good for simultaneous mapping of APs and intracellular Ca2+.Not commercially available
RH-155
(NK3041)
650-720nmCa2+- and K+-dependent communication between central nervous system myelinated axons and oligodendrocytes revealed by voltage-sensitive dyes
Proc Natl Acad Sci U S A . 1986 Sep;83(17):6651-5.

Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures
Nat Protoc. 2010 Mar; 5(3): 439-456.
Absorption dye

High affinity for glial cell membrane
- Santa Cruz Biotechnology

- AAT Bioquest
RH-482
(NK3630/
JPW1132)
700-705nmOptical recording of electrical activity from parallel fibres and other cell types in skate cerebellar slices in vitro
J Physiol. 1987 Dec; 393: 681-702.

High precision and fast functional mapping of cortical circuitry through a novel combination of voltage sensitive dye imaging and laser scanning photostimulation
J Neurophysiol. 2010 Apr; 103(4): 2301-2312.
Absorption dyeNot commercially available
(上記の表は一般的な情報です。最適な波長は実験条件によって異なります。)