Rat In Vivo Imaging using Voltage-Sensitive Dye (MiCAM02-HR)

Rat In Vivo Imaging using Voltage-Sensitive Dye (MiCAM02-HR)


Animal
Rat
Sample
In Vivo (Insular Cortex)
Method
Electrical stimulation (a train of 5 stimuli with 20msec interval)
Dye
RH1691
Sampling Rate
4.0ms/frame
Averaging
15 times
Provided by
Dr.Satoshi Fujita and Dr.Masayuki Kobayashi
Nihon University School of Dentistry, Japan
Reference
Fujita S, Adachi K, Koshikawa N, Kobayashi M.
Spatiotemporal dynamics of excitation in rat insular cortex: intrinsic corticocortical circuit regulates caudal-rostro excitatory propagation from the insular to frontal cortex.
Neuroscience. 2009 Oct 1. [Epub ahead of print]

Mouse In Vivo Imaging using Voltage-Sensitive Dye (MiCAM ULTIMA)

Mouse In Vivo Imaging using Voltage-Sensitive Dye


Voltage-sensitive dye imaging of in-vivo mouse with MiCAM ULTIMA.
This data was obtained from a 2 month-old mouse that was under urethane anaesthesia (1.75 g/kg). A heating pad was used to maintain the measured body temperature of the mouse at 37°C. Two electrodes were inserted under the skin of the forearms to receive the electrocardiogram signal which was used to trigger the MiCAM ULTIMA acquisition.

Animal
Mouse
Sample
In Vivo (Somatosensory Barrel Cortex)
Method
Piezo Whisker Flick Stimulation
Dye
RH1691
Sampling Rate
2.0ms/frame
Averaging
10 times
Provided by
Dr.Isabelle Ferezou and Dr.Carl C.H. Petersen
Brain Mind Institute, EPFL, Switzerland
Reference
Visualizing the Cortical Representation of Whisker Touch: Voltage-Sensitive Dye Imaging in Freely Moving Mice.
Neuron. 2006 May 18;50(4):617-29.

高速イメージングシステム MiCAM03-N256

MiCAM03-N256は膜電位感受性色素、カルシウム色素などの蛍光プローブで染色された生体サンプル内の微弱な蛍光輝度変化を高速で捉え、画像化することのできる世界最高性能の光計測システムです。

これまで世界中の脳神経/心臓研究分野で使用され、多くの実績を残したブレインビジョン製MiCAMシリーズの標準モデルである高速イメージングシステムMiCAM02の後継機種となります。

高速撮影・高S/N比、直感的な操作方法などの基本性能は継承しつつ、新開発CMOSカメラヘッドによる高画素化、高速データ転送による長時間記録などの新たな特長が加えられ、これまでには無いイメージングシステムへと進化を遂げました。