Monolayers of neonatal mouse ventricular myocyte stained with voltage sensitive dye

Sample
Monolayer of neonatal mouse ventricular myocyte. Sample was scratched with a needle tip at three locations (visible on the fluorescence pictures) to induce conduction blocks and meandering of the wavefront.
Method
Sample is paced locally at different frequencies using a glass microelectrode positioned at the edge of the sample
Fluorescence Dye
Voltage sensitive dye (Di-8-Anepps)
Imaging System
MiCAM05-N256
Pixels
256×256
Frame Rate
1,000fps (1.0msec/frame)
Provided by
Dr. Jan Kucera and Dr. Ange Maguy, Department of Physiology, University of Bern

Cultured Neonatal Rat Ventricular Cardiomyocytes (MiCAM ULTIMA-L)

Cultured Neonatal Rat Ventricular Cardiomyocytes (MiCAM ULTIMA-L)


Sustained spiral wave reentry in a 3 day old monolayer disc of cultured neonatal rat ventricular cardiomyocytes (frequency ~ 5 Hz; diameter of the preparation = 8 mm). The preparation was stained with the voltage sensitive dye di-8-ANEPPS and activity was recorded at 1,000 frames/second using a custom-made tandem lens macroscope and an Ultima-L camera system.

Provided by
Dr.Stephan Rohr, University of Bern

Calcium Imaging in Cultured Cardiomyocytes (Fluo-4)

Calcium Imaging in Cultured Cardiomyocytes (Fluo-4)


The preparation was cultured rat cardiomyocytes stained with Fluo-4 and activity was recorded using MiCAM01 (old model) in 2002. The current model, MiCAM02 can significantly improve the data in terms of spatial resolution, sensitivity and S/N ratio.


高速イメージングシステム MiCAM03-N256

MiCAM03-N256は膜電位感受性色素、カルシウム色素などの蛍光プローブで染色された生体サンプル内の微弱な蛍光輝度変化を高速で捉え、画像化することのできる世界最高性能の光計測システムです。

これまで世界中の脳神経/心臓研究分野で使用され、多くの実績を残したブレインビジョン製MiCAMシリーズの標準モデルである高速イメージングシステムMiCAM02の後継機種となります。

高速撮影・高S/N比、直感的な操作方法などの基本性能は継承しつつ、新開発CMOSカメラヘッドによる高画素化、高速データ転送による長時間記録などの新たな特長が加えられ、これまでには無いイメージングシステムへと進化を遂げました。